[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-332789-105":59,"doc-detail-332789-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","integrated-mirna-signatures-advancing-breast-cancer-diagnosis-and-prognosis","Integrated miRNA Signatures: Advancing Breast Cancer Diagnosis and Prognosis","","Breast cancer is the leading cause of incidence worldwide and the second cause of cancer deaths, with rising mortality despite existing screening. Imaging and invasive biopsies are used for detection but can lack sufficient sensitivity and specificity. MicroRNAs can function as diagnostic and prognostic biomarkers. A computational workflow integrating transcriptome profiling, survival analysis, and diagnostic power estimation was built from 1165 TCGA-BRCA patients, producing two miRNA signatures.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/integrated-mirna-signatures-advancing-breast-cancer-diagnosis-and-prognosis/332789/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/integrated-mirna-signatures-advancing-breast-cancer-diagnosis-and-prognosis/332789.png","ImageObject",300,407,{"name":92,"@type":93},"Seraphina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why are new biomarkers needed for breast cancer diagnosis and prognosis?","Question",{"text":112,"@type":113},"Current screening relies on imaging and biopsies, which may not achieve high sensitivity and specificity. This motivates more reliable molecular biomarkers.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the miRNA signatures developed in the study?",{"text":117,"@type":113},"They were derived using a computational approach that combines transcriptome profiling, survival analyses, and diagnostic power calculations using TCGA-BRCA data.",{"name":119,"@type":110,"acceptedAnswer":120},"What is the clinical significance of the two miRNA signatures?",{"text":121,"@type":113},"The two signatures show strong diagnostic performance and are linked to statistically significant prognostic effects on overall survival and lymph-node invasion in breast cancer patients.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332789,1790197003,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":56,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962075114101,"https://ap-avatar.wpscdn.com/avatar/e000253a75eb197efd?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780044092746381165","biomolecules  \nArticle  \nIntegrated miRNA Signatures: Advancing Breast Cancer Diagnosis and Prognosis  \nMaria Samara 1,†, Eleni Thodou 1,†, Marina Patoulioti 2, Antigoni Poultsidi 3, Georgia Eleni Thomopoulou 4 and Antonis Giakountis 2, *  \nCitation: Samara, M.; Thodou, E.; Patoulioti, M.; Poultsidi, A.;  \nThomopoulou, G.E.; Giakountis, A. Integrated miRNA Signatures: Advancing Breast Cancer Diagnosis and Prognosis. Biomolecules 2024, 14, 1352. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)biom14111352  \nAcademic Editor: Tracey Martin  \nReceived: 26 August 2024  \nRevised: 15 October 2024  \nAccepted: 23 October 2024  \nPublished: 24 October 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Pathology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Biopolis, Mezourlo, 41500 Larissa, Greece  \n2 Department of Biochemistry and Biotechnology, University of Thessaly, Biopolis, Mezourlo,  \n41500 Larissa, Greece  \n3 Surgical Department, Faculty of Medicine, University Hospital of Larissa, University of Thessaly, Biopolis, Mezourlo, 41500 Larissa, Greece  \n4 Diagnostic Cytopathology Department, Attikon General Hospital, School of Medicine, The National and Kapodistrian University of Athens, 11527 Athens, Greece  \n* Correspondence: [agiakountis@uth.gr](agiakountis@uth.gr)[ ](agiakountis@uth.gr)† These authors contributed equally to this work.  \nAbstract: Breast cancer ranks first in incidence and second in deaths worldwide, presenting alarmingly rising mortality rates. Imaging methodologies and/or invasive biopsies are routinely used for screening and detection, although not always with high sensitivity/specificity. MicroRNAs (miRNAs) could serve as diagnostic and prognostic biomarkers for breast cancer. We have designed a computational approach combining transcriptome profiling, survival analyses, and diagnostic power calculations from 1165 patients with breast invasive carcinoma from The Cancer Genome Atlas (TCGA-BRCA). Our strategy yielded two separate miRNA signatures consisting of four up-regulated and five down-regulated miRNAs in breast tumors, with cumulative diagnostic strength of AUC 0.93 and 0.92, respectively. We provide direct evidence regarding the breast cancer-specific expression of both signatures through a multicancer comparison of >7000 biopsies representing 19 solid cancer types, challenging their diagnostic potency beyond any of the current diagnostic methods. Our signatures are functionally implicated in cancer-related processes with statistically significant effects on overall survival and lymph-node invasion in breast cancer patients, which underlie their strong prognostic implication. Collectively, we propose two novel miRNA signatures with significantly elevated diagnostic and prognostic power as a functionally resolved tool for binary and accurate detection of breast cancer and other tumors of the female reproductive system.  \nKeywords: miRNA; breast cancer; biomarker; diagnosis; prognosis; multi-cancer transcriptomics; ROC  \n1. Introduction  \nBreast invasive carcinoma (BRCA) is the most frequent type of cancer in females and the fourth leading cause of cancer-related deaths for both sexes worldwide. In 2022, there were 2 .3 million new cases and more than 650,000 deaths. France, Australia, New Zealand, Northern Europe, and the Northern USA exhibit the highest incidence rates, while Fiji Island, Western Africa, and Polynesia display the highest mortality rates, underscoring the geographical heterogeneity of the disease [1] . Female breast cancer is heterogeneous, with distinct molecular subtypes and clinical outcomes [2] . The ","cbCain8VecBWK3c0","https://ap.wps.com/l/cbCain8VecBWK3c0","pdf",4172495,"English","# Abstract\n# Keywords\n# Introduction","[{\"question\":\"Why are new biomarkers needed for breast cancer diagnosis and prognosis?\",\"answer\":\"Current screening relies on imaging and biopsies, which may not achieve high sensitivity and specificity. This motivates more reliable molecular biomarkers.\"},{\"question\":\"How were the miRNA signatures developed in the study?\",\"answer\":\"They were derived using a computational approach that combines transcriptome profiling, survival analyses, and diagnostic power calculations using TCGA-BRCA data.\"},{\"question\":\"What is the clinical significance of the two miRNA signatures?\",\"answer\":\"The two signatures show strong diagnostic performance and are linked to statistically significant prognostic effects on overall survival and lymph-node invasion in breast cancer patients.\"}]","Integrated miRNA Signatures: Advancing Breast Cancer Diagnosis and Prognosis | PDF",1789999513,48]