[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-331530-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-331530-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","inhibition-of-npc1-suppresses-cell-proliferation-and-catenin-signaling-activation-of-liver-cancer","Inhibition of NPC1 suppresses cell proliferation and β-catenin signaling activation of liver cancer","","Niemann-Pick Type C1 (NPC1) is investigated for its role in regulating liver cancer development. Patient tumor data show that higher NPC1 expression correlates with poorer prognosis. In vitro inhibition of NPC1 reduces proliferation, invasion, and migration of liver cancer cells and triggers apoptosis, accompanied by decreased Wnt/β-catenin signaling activation. In vivo results further confirm that suppressing NPC1 effectively restrains liver cancer cell growth. NPC1 is proposed as a promising therapeutic target.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/inhibition-of-npc1-suppresses-cell-proliferation-and-catenin-signaling-activation-of-liver-cancer/331530/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/inhibition-of-npc1-suppresses-cell-proliferation-and-catenin-signaling-activation-of-liver-cancer/331530.png","ImageObject",300,407,{"name":42,"@type":43},"Quinn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-27","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the study’s objective regarding NPC1 in liver cancer?","Question",{"text":63,"@type":64},"To investigate how NPC1 is involved in regulating liver cancer development.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does inhibiting NPC1 affect liver cancer cells in vitro?",{"text":68,"@type":64},"Inhibiting NPC1 reduces proliferation, invasion, and migration, and induces apoptosis.",{"name":70,"@type":61,"acceptedAnswer":71},"What signaling pathway is linked to NPC1 suppression in this study?",{"text":72,"@type":64},"NPC1 inhibition decreases activation of Wnt/β-catenin signaling.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},331530,1790159996,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},962075114765,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Journal of Cancer Research and Clinical Oncology (2024) 150:498  \n[https://doi.org/10.1007/s00432-024-06023-7](https://doi.org/10.1007/s00432-024-06023-7)  \nRESEARCH  \nInhibition of NPC1 suppresses cell proliferation and β-catenin signaling activation of liver cancer  \nDan Cao1,2,3 · Han Chen1,2 · Min He1,2 · Ning Han1,2 · Hong Tang1,2  \nReceived: 26 June 2024 / Accepted: 1 November 2024 / Published online: 15 November 2024 © The Author(s) 2024  \nAbstract  \nNiemann-Pick Type C1 (NPC1) plays a significant role in the development of liver diseases and liver cancer. Our objective was to investigate the involvement of NPC1 in regulating liver cancer development. We observed that high levels of NPC1 expression in tumor tissues from patients with liver cancer were associated with a poor prognosis. Through in vitro experiments, we found that inhibiting NPC1 expression reduced the proliferation, invasion, and migration of liver cancer cells, while also inducing apoptosis in these cells. Additionally, the inhibition of NPC1 led to decreased activation of Wnt/β-catenin signaling. In vivo studies further supported our findings by demonstrating that the suppression of liver cancer cell growth was effectively achieved through the inhibition of NPC1 . Overall, our results strongly indicate that the inhibition of NPC1 suppresses liver cancer cell proliferation. Targeting NPC1 is a promising potential therapeutic strategy for liver cancer.  \nKeywords Liver cancer · NPC1 · Wnt/β-catenin signaling  \nIntroduction  \nLiver cancer is a prevalent malignant tumor characterized by atypical clinical symptoms in its early stages, rapid progression, and high mortality rate. Cholesterol and its metabolites (its precursors and derivatives) play important roles in cancer (Xu et al. 2020) . In recent years, numerous studies have reported the functions of cholesterol metabolism in the regulation of tumor biological processes, especially oncogenic signaling pathways, ferroptosis, and tumor microenvironment (Kuzu et al. 2016; Huang et al. 2020; Xu et  \nDan Cao and Han Chen contributed equally to this work and should be considered co-first authors.  \n􀀍 Hong Tang [tanghong6198@wchscu.cn](tanghong6198@wchscu.cn)  \n1 Center of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China  \n2 Laboratory of Infectious and Liver Diseases, Institute of Infectious Diseases, West China Hospital of Sichuan University, Chengdu 610041, China  \n3 Department of Respiratory and Critical Care Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China  \nal. 2020) . Some new cholesterol metabolic molecules such as sterol-O-acyltransferase 1(SOAT1), squalene epoxidase (SQLE), and Niemann-Pick Type C1(NPC1) have recently been reported to be involved in cancer progression (Chidambaranathan-Reghupaty et al. 2021; Zhu et al. 2021; Liet al. 2022) . NPC1 is a transmembrane glycoprotein composed of 1278 amino acids and is primarily located on the limiting membrane of the late endosome/lysosome (Ioannou 2000; Vanier and Millat 2003) . In recent years, studies have identified upregulated expression of NPC1 in various tumor tissues, including breast cancer, cervical cancer, glioma and HCC (Du et al. 2015; Lee et al. 2022; O’Neill et al. 2022; Xu et al. 2024) . Increased NPC1 expression has been found to significantly enhances tumor cell proliferation, migration, invasion and autophagy. Furthermore, some analysis has revealed the possible correlation between elevated NPC1 expression and unfavorable prognosis in liver cancer patients (Wang et al. 2022; Hwang et al. 2023; Xu et al. 2024) . In this study, we revealed the expression of NPC1 in liver cancer and its role of NPC1 in liver cancer development.  \nResults  \nNPC1 was found to be elevated in liver cancer tissues  \nTo investigate the expression of NPC1 in liver cancer tissues, we searched databases for information on NPC1 expression in various cancers. Analysis of RNA-Seq data  \nfrom the","cbCaioOGsqIk2EBw","https://ap.wps.com/l/cbCaioOGsqIk2EBw","pdf",10261793,13,"English","# Abstract\n# Introduction\n# Results\n## NPC1 was found to be elevated in liver cancer tissues","[{\"question\":\"What was the study’s objective regarding NPC1 in liver cancer?\",\"answer\":\"To investigate how NPC1 is involved in regulating liver cancer development.\"},{\"question\":\"How does inhibiting NPC1 affect liver cancer cells in vitro?\",\"answer\":\"Inhibiting NPC1 reduces proliferation, invasion, and migration, and induces apoptosis.\"},{\"question\":\"What signaling pathway is linked to NPC1 suppression in this study?\",\"answer\":\"NPC1 inhibition decreases activation of Wnt/β-catenin signaling.\"}]","Inhibition of NPC1 suppresses cell proliferation and β-catenin signaling activation of liver cancer | PDF",1789993774,33]