[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-381688-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-381688-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","influence-of-clinical-factors-on-mir-3613-3p-expression-in-colorectal-cancer","Influence of Clinical Factors on miR-3613-3p Expression in Colorectal Cancer","","Colorectal cancer (CRC) is a leading cause of cancer mortality worldwide, largely because many cases present at an advanced, asymptomatic stage. Several microRNAs may serve as noninvasive diagnostic and prognostic biomarkers, yet functional evidence for miR-3613-3p is limited and findings have been inconsistent. This pilot microarray study identified reduced miR-3613-3p in CRC cells, followed by qPCR validation in 83 patients, showing decreased expression in both malignant and benign tumors versus adjacent tissue. Radiotherapy was associated with higher miR-3613-3p in surrounding tissues, and increased expression was also observed in patients with distant metastases, supporting further study of miR-3613-3p as a potential therapeutic target.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/influence-of-clinical-factors-on-mir-3613-3p-expression-in-colorectal-cancer/381688/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/influence-of-clinical-factors-on-mir-3613-3p-expression-in-colorectal-cancer/381688.png","ImageObject",300,407,{"name":42,"@type":43},"Pentious","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-24",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",9,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What clinical factors and patient groups were analyzed regarding miR-3613-3p?","Question",{"text":63,"@type":64},"The study evaluated miR-3613-3p expression across 83 patients, including colorectal cancer, benign tumor, and control groups, and compared tumor tissues with surrounding tissue. It also compared patients receiving radiotherapy versus those without and assessed distant metastasis status.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was miR-3613-3p expression measured and validated?",{"text":68,"@type":64},"A pilot microarray study first suggested decreased miR-3613-3p in colorectal cancer cells. Expression was then confirmed using qPCR on the patient cohort.",{"name":70,"@type":61,"acceptedAnswer":71},"What did radiotherapy and distant metastasis show in relation to miR-3613-3p?",{"text":72,"@type":64},"Colorectal tumor patients who received radiotherapy before surgery had significantly higher miR-3613-3p expression in surrounding tissues than those who did not receive radiotherapy. Surrounding tissue from patients with distant metastases showed significantly higher miR-3613-3p than those without distant metastases.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},381688,1790409013,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},1374404730887,"https://ap-avatar.wpscdn.com/davatar_6f874abed73319feea01a86fa6f0fab8","Communication  \nInfluence of Clinical Factors on miR-3613-3p Expression in Colorectal Cancer  \nPaulina Gil-Kulik 1, *,†, Alicja Petniak 1, *,†, Natalia Kluz 1, Grzegorz Wallner 2, Tomasz Skoczylas 2, Aleksander Ciecha ´nski 2,‡ and Janusz Kocki 1,‡  \nCitation: Gil-Kulik, P.; Petniak, A.; Kluz, N.; Wallner, G.; Skoczylas, T.;  \nCiecha ´nski, A.; Kocki, J. Influence of Clinical Factors on miR-3613-3p Expression in Colorectal Cancer. Int.  \nJ. Mol. Sci. 2023, 24, 14023. [https://](https://)[ ](https://)[doi.org/10.3390/ijms241814023](doi.org/10.3390/ijms241814023)  \nAcademic Editor: Simona Gurzu  \nReceived: 1 August 2023  \nRevised: 7 September 2023  \nAccepted: 11 September 2023  \nPublished: 13 September 2023  \nCopyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, Poland; [nataliakluz99@gmail.com](nataliakluz99@gmail.com) (N.K.); [janusz.kocki@umlub.pl](janusz.kocki@umlub.pl) (J.K.)  \n2 II Chair and Department of General and Gastrointestinal Surgery and Surgical Oncology of the Alimentary Tract, 16 Staszica Str., 20-081 Lublin, Poland; [grzegorz.wallner@umlub.pl](grzegorz.wallner@umlub.pl) (G.W.); [tomasz.skoczylas@umlub.pl](tomasz.skoczylas@umlub.pl) (T.S.); [aleksander.ciechanski@umlub.pl](aleksander.ciechanski@umlub.pl) (A.C.)  \n* [Correspondence: pgil.poczt@vp.pl](Correspondence: pgil.poczt@vp.pl) (P.G.-K.); [alicja.petniak@umlub.pl](alicja.petniak@umlub.pl) (A.P.)† These authors contributed equally to this work (first authorship) .  \n‡ These authors contributed equally to this work (senior authorship) .  \nAbstract: Colorectal cancer (CRC) is the second most common cause of cancer-related death globally. Because of a tendency to be an asymptomatic primary tumor and therefore resulting in late detection, most CRC patients are diagnosed in the advanced stage. Several miRNAs have the potential to become novel noninvasive biomarkers measured as diagnostic and prognostic indicators of CRC to guide surgical therapies and promote the understanding of the carcinogenesis of CRC. Since the change of miR-3613-3p was associated with several types of cancer other than colorectal cancer, thereis a lack of functional evidence and the results are inconsistent. We conducted a pilot microarray study in which we noted a decreased expression of miR-3613-3p in colorectal cancer cells, then we confirmed the expression of miR-3613-3p by qPCR on a group of 83 patients, including 65 patients with colorectal cancer, 5 with a benign tumor and 13 from the control group. We noted that in both malignant and benign tumors, miR-3613-3p is downgraded relative to the surrounding tissue. As a result of the study, we also observed colorectal tumor tissue and surrounding tissue in patients with colorectal cancer who received radiotherapy before surgery, which showed a significantly higher expression of miR-3613-3p compared to patients who did not receive radiotherapy. In addition, we noted that the tissue surrounding the tumor in patients with distant metastases showed a significantly higher expression of miR-3613-3p compared to patients without distant metastases. The increased expression of miR-3613-3p in patients after radiotherapy suggests the possibility of using this miR asa therapeutic target for CRC, but this requires confirmation in further studies.  \nKeywords: colorectal cancer; microRNA; gene expression regulation; miR-3613-3p  \n1. Introduction  \nColorectal cancer (CRC) is the second most common cause of cancer-related death globally [1] . In 2020, there were an estimated 1.93 million new cases of CRC and an estimated 0 .94 million disease-related deaths wo","cbCaiinIzg8a4wZq","https://ap.wps.com/l/cbCaiinIzg8a4wZq","pdf",1999982,14,"English","# Abstract\n# Introduction\n## CRC burden and staging\n## Clinical and molecular factors\n## Consensus molecular subtypes","[{\"question\":\"What clinical factors and patient groups were analyzed regarding miR-3613-3p?\",\"answer\":\"The study evaluated miR-3613-3p expression across 83 patients, including colorectal cancer, benign tumor, and control groups, and compared tumor tissues with surrounding tissue. It also compared patients receiving radiotherapy versus those without and assessed distant metastasis status.\"},{\"question\":\"How was miR-3613-3p expression measured and validated?\",\"answer\":\"A pilot microarray study first suggested decreased miR-3613-3p in colorectal cancer cells. Expression was then confirmed using qPCR on the patient cohort.\"},{\"question\":\"What did radiotherapy and distant metastasis show in relation to miR-3613-3p?\",\"answer\":\"Colorectal tumor patients who received radiotherapy before surgery had significantly higher miR-3613-3p expression in surrounding tissues than those who did not receive radiotherapy. Surrounding tissue from patients with distant metastases showed significantly higher miR-3613-3p than those without distant metastases.\"}]","Influence of Clinical Factors on miR-3613-3p Expression in Colorectal Cancer | PDF",1790246503,35]