[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-331729-105":3,"detail-sidebar-cat-0-en-105":85,"doc-detail-331729-en":135},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":78,"head_meta":80,"extra_data":82,"updated_unix":84},105,"en","indel-instability-in-two-different-tumoral-tissues-and-its-forensic-significance","InDEL instability in two different tumoral tissues and its forensic significance","","Malignant tumor samples can be used for forensic DNA profiling when reference material is unavailable. STRs are widely used for identification, yet tumoral genetic instability can alter STR polymorphisms, producing erroneous results. InDel polymorphisms provide alternative markers because they are suitable for degraded DNA and can be more discriminative than STRs alone. This study assessed whether InDel instability occurs in formalin-fixed paraffin-embedded breast and thyroid tumor tissues using a 36plex InDel panel. Results from 47 cases showed higher mutational changes in breast cancer than thyroid carcinoma, with loss of heterozygosity as a common event, supporting careful forensic use of FFPE tissues and improving discrimination by adding low-mutation markers.",{"@graph":14,"@context":77},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/indel-instability-in-two-different-tumoral-tissues-and-its-forensic-significance/331729/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/indel-instability-in-two-different-tumoral-tissues-and-its-forensic-significance/331729.png","ImageObject",300,407,{"name":42,"@type":43},"Valentina","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69,73],{"name":60,"@type":61,"acceptedAnswer":62},"Why can tumor tissues cause errors in forensic DNA profiling using STRs?","Question",{"text":63,"@type":64},"Tumor genetic instability can change STR polymorphisms, leading to discordant genetic profiles and potentially erroneous identification results.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What was the study designed to determine and which panel was used?",{"text":68,"@type":64},"The study investigated whether instability occurs in tumoral tissues using a 36plex InDel panel and compared InDel profiles from tumoral and non-tumoral tissues.",{"name":70,"@type":61,"acceptedAnswer":71},"How did InDel instability differ between breast and thyroid tumors in the results?",{"text":72,"@type":64},"Breast cancers showed mutational changes in most cases (80.76%), while thyroid carcinoma displayed instability in a smaller fraction (28.57%).",{"name":74,"@type":61,"acceptedAnswer":75},"What is the forensic implication of using FFPE tumor tissues for genetic identification?",{"text":76,"@type":64},"The findings suggest thyroid FFPE tissues can be used for forensic genetic identification, but breast FFPE tissues require careful examination, as reference profiles may differ from actual cases. Adding markers such as SNPs and microhaplotypes with low mutation rates can enhance discrimination power.","https://schema.org",{"og:url":32,"og:type":79,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":81,"canonical":32},"index,follow",{"doc_id":83,"site_id":7},331729,1790461076,{"code":4,"msg":86,"data":87},"success",[88,92,96,100,105,110,115,119,124,127,131],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},"Exam",70,"exam",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},5,"Comic",60,"comic",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},6,"Technology",50,"technology",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},7,"Healthcare",40,"healthcare",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":117,"slug":118},8,30,"research-report",{"id":120,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":122,"slug":123},9,"Religion & Spirituality",20,"religion-spirituality",{"id":122,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":122,"slug":126},"World Cup","world-cup",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":128,"slug":130},10,"Lifestyle","lifestyle",{"id":132,"doc_module":4,"doc_module_name":25,"category_name":133,"show_sort_weight":101,"slug":134},19,"General","general",{"code":4,"msg":86,"data":136},{"doc_id":83,"user_id":137,"nickname":42,"user_avatar":138,"doc_module":4,"category_id":116,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":139,"file_id":140,"file_url":141,"file_type":142,"file_size":143,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":128,"language":144,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":12,"update_tm":148,"read_time":149,"preview_page_count":4},13056703020460,"https://ap-avatar.wpscdn.com/avatar/be000253dac470eee5d?_k=1778207105932848923","Forensic Science, Medicine and Pathology (2024) 20:1241–1250  \n[https://doi.org/10.1007/s12024-024-00808-5](https://doi.org/10.1007/s12024-024-00808-5)  \nInDEL instability in two different tumoral tissues and its forensic significance  \nİpek Gürel1,2 · Faruk Aşıcıoğlu3 · Gökhan Ersoy3 · Özlem Bülbül1 · Tülin Öztürk4 · Gönül Filoğlu1  \nAccepted: 22 March 2024 / Published online: 3 April 2024 © The Author(s) 2024  \nAbstract  \nThere may be cases where malignant tumor samples can be used for forensic DNA profiling studies. STRs are the first systems preferred in forensic science laboratories for identification purposes. However, genetic instability in tumoral tissues causes STR polymorphism to change, leading to erroneous results. On the other hand, insertion/deletion polymorphism (InDels) are used as genetic markers in forensic science, as they have features that make both STR and SNPs preferable. Although previous studies approved that STR instability is observed in many different tumors, there are only a few studies that have displayed the instability of InDels in tumoral tissues before. In this study, it was aimed to determine whether instability is observed in formalin-fixed paraffin-embedded breast and thyroid tumoral tissues at 36plex InDel Panel. A total of 47 cases, 26 of which were diagnosed as breast cancer and 21 as thyroid cancer, were included in the study. In 21 of 26 (80.76%) breast cancers mutational changes were observed, however only 6 of 21 (28.57%) thyroid carcinoma cases displayed instability. Moreover, in these six cases, mutations were detected at only 1 or 2 loci. The most common change in both tissues was loss of heterozygosity. These findings suggest that paraffin embedded tissues of thyroid tumor can be used in cases of forensic genetic identification, however paraffin embedded breast cancer tissues should be examined with care. In conclusion, low InDel mutation rates compared to STR instability, make InDel analysis from paraffin blocks suitable for forensic genetic identification. However, researchers should keep in mind that there may be differences between the profiles of the tumoral tissues taken as reference and the actual case. In addition, by incorporating additional markers such as SNPs and microhaplotypes with low mutation rates into the study alongside Indels, researchers can significantly enhance the discrimination power in identification processes.  \nKeywords InDels · Instability · Tumoral tissue · Identification · STR · Forensic genetics  \n* Faruk Aşıcıoğlu  \n[fasicioglu@hotmail.com](fasicioglu@hotmail.com); [faruk.asicioglu@iuc.edu.tr](faruk.asicioglu@iuc.edu.tr)  \n1 Department of Science, Institute of Forensic Sciences and Legal Medicine, İstanbul University-Cerrahpaşa,İstanbul, Türkiye  \n2 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Haliç University, İstanbul, Türkiye  \n3 Department of Medical Sciences, Institute of Forensic Sciences and Legal Medicine, İstanbul University-Cerrahpaşa, İstanbul, Türkiye  \n4 Department of Medical Pathology, Cerrahpaşa Faculty of Medicine, İstanbul University-Cerrahpaşa, İstanbul, Türkiye  \nIntroduction  \nAnalysis of DNA polymorphisms in humans is a valuable tool for forensic identification, if there is a reference sample to compare with the sample in question. Where there is no reliable reference sample, it is necessary to find an alternative source such as an histopathology specimen. Some difficulties may arise when the accuracy of the reference sample is questionable with possible genetic variability, such as malignant tumors [1] . This is because tumor tissues generally have mutations, which may affect genotype analysis. However, there are some situations where the use of tumor tissues may be the most advantageous option [2–4] .  \nThe reason why STR loci are the first preferred method in forensic science laboratories is that there are approximately 1 million STR loci in the genome, and they show high polymorphism due to t","cbCaisRDHAeocjV1","https://ap.wps.com/l/cbCaisRDHAeocjV1","pdf",906310,"English","# Abstract\n## Background\n## STRs and genetic instability\n## InDels as forensic markers\n## Study aim and approach","[{\"question\":\"Why can tumor tissues cause errors in forensic DNA profiling using STRs?\",\"answer\":\"Tumor genetic instability can change STR polymorphisms, leading to discordant genetic profiles and potentially erroneous identification results.\"},{\"question\":\"What was the study designed to determine and which panel was used?\",\"answer\":\"The study investigated whether instability occurs in tumoral tissues using a 36plex InDel panel and compared InDel profiles from tumoral and non-tumoral tissues.\"},{\"question\":\"How did InDel instability differ between breast and thyroid tumors in the results?\",\"answer\":\"Breast cancers showed mutational changes in most cases (80.76%), while thyroid carcinoma displayed instability in a smaller fraction (28.57%).\"},{\"question\":\"What is the forensic implication of using FFPE tumor tissues for genetic identification?\",\"answer\":\"The findings suggest thyroid FFPE tissues can be used for forensic genetic identification, but breast FFPE tissues require careful examination, as reference profiles may differ from actual cases. Adding markers such as SNPs and microhaplotypes with low mutation rates can enhance discrimination power.\"}]","InDEL instability in two different tumoral tissues and its forensic significance | PDF",1789994907,25]