[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-462791-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-462791-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","increased-intrahepatic-mast-cell-density-in-liver-cirrhosis-due-to-masld-and-other-non-infectious-chronic-liver-diseases","Increased Intrahepatic Mast Cell Density in Liver Cirrhosis - Due to MASLD and Other Non-Infectious Chronic Liver Diseases","","Metabolic dysfunction-associated steatotic liver disease (MASLD) is highly prevalent, yet its mechanisms of progression remain incompletely defined. Chronic hepatic inflammation and regeneration activate innate immune cells, including mast cells (MCs), whose involvement in MASLD-related cirrhosis is insufficiently studied. A retrospective count of tryptase-positive MCs in cirrhotic liver explants compared MASLD, alcohol-related liver disease (ALD), and autoimmune hepatitis (AIH) versus controls, using Kruskal–Wallis with Dunn’s tests. MC density was significantly higher in MASLD, ALD, and AIH, with ALD exceeding AIH, and correlated with tobacco and alcohol use.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/increased-intrahepatic-mast-cell-density-in-liver-cirrhosis-due-to-masld-and-other-non-infectious-chronic-liver-diseases/462791/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/increased-intrahepatic-mast-cell-density-in-liver-cirrhosis-due-to-masld-and-other-non-infectious-chronic-liver-diseases/462791.png","ImageObject",300,407,{"name":42,"@type":43},"Mimi","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-03","2026-09-30",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",10,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the study’s main aim regarding mast cells?","Question",{"text":63,"@type":64},"To evaluate differences in mast cell (MC) density in cirrhotic liver tissue among patients with MASLD and other chronic liver disease etiologies.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were mast cells identified and quantified?",{"text":68,"@type":64},"Tryptase-positive mast cells were identified by indirect immunofluorescence and quantified as MC density per low-power field (MC/LPF).",{"name":70,"@type":61,"acceptedAnswer":71},"What clinical factors were associated with higher mast cell infiltration?",{"text":72,"@type":64},"MC density showed a positive correlation with tobacco smoking and alcohol use in the full analyzed group.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},462791,1790796662,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,115,120,123,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":113,"slug":114},8,"Research & Report",30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":55,"slug":125},"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":55,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":143,"read_time":144,"preview_page_count":4},2336477974920,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Brief Report  \nIncreased Intrahepatic Mast Cell Density in Liver Cirrhosis  \nDue to MASLD and Other Non-Infectious Chronic Liver Diseases  \nNicolás Ortiz-López 1,2,†, Araceli Pinto-León 1,†, Javiera Favi 1, Dannette Guíñez Francois 3, Larissa Aleman 1,2  \n,  \nLaura Carreño-Toro 4, Alejandra Zazueta 5,6, Fabien Magne 5, Jaime Poniachik 3 and Caroll J. Beltrán 1,7, *  \nAcademic Editor: LiliyaMihaylova  \nReceived: 19 September 2025  \nRevised: 11 November 2025  \nAccepted: 20 November 2025  \nPublished: 30 December 2025  \nCopyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.  \n1 Laboratory of Immunogastroenterology I, Section of Gastroenterology, Department of Medicine, Hospital Clínico Universidad de Chile, Santiago 8380456, Chile; [nicolas.ortiz@ug.uchile.cl](nicolas.ortiz@ug.uchile.cl) (N.O.-L.); [aracelipleon@gmail.com](aracelipleon@gmail.com) (A.P.-L.); [javiera.favi@ug.uchile.cl](javiera.favi@ug.uchile.cl) (J.F.); [larialempy@gmail.com](larialempy@gmail.com) (L.A.)  \n2 Graduate School, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile  \n3 Section of Gastroenterology, Department of Medicine, Hospital Clínico Universidad de Chile, Santiago 8380456, Chile; [dannette.vania@uchile.cl](dannette.vania@uchile.cl) (D.G.F.); [jponiachik@hcuch.cl](jponiachik@hcuch.cl) (J.P.)  \n4 Department of Pathology, Hospital Clínico Universidad de Chile, Santiago 8380456, Chile; [lcarrenotoro@gmail.com](lcarrenotoro@gmail.com)  \n5 Program of Microbiology and Mycology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile; [alezazueta28@gmail.com](alezazueta28@gmail.com) (A.Z.); [fmagne@uchile.cl](fmagne@uchile.cl) (F.M.)  \n6 Medical Technology, Faculty of Medicine Clínica Alemana, Universidad del Desarrollo, Santiago 7630426, Chile  \n7 Laboratory of Immunogastroenterology II, Department of Clinical Biochemistry and Immunology, Faculty of Pharmacy, Universidad de Concepción, Concepción 4070386, Chile  \n* Correspondence: [carollbeltranm@uchile.cl](carollbeltranm@uchile.cl); Tel.: +56-9-78987-679 † These authors contributed equally to this work.  \nAbstract  \nMetabolic dysfunction-associated steatotic liver disease (MASLD) has become highly prevalent worldwide, and its pathogenesis and progression mechanisms remain incompletely understood. An increased activation of innate immune cells in the liver contributes to hepatic fibrogenesis via a chronic loop of inflammation and regeneration processes. Among them are mast cells (MCs), whose role in hepatic cirrhosis secondary to MASLD remains poorly studied. Our aim was to evaluate differences in MC density in cirrhotic liver tissue among patients with MASLD and other chronic liver disease etiologies. For this, a retrospective study of MC count was performed in cirrhotic liver explants obtained from MASLD, alcohol-related liver disease (ALD), and autoimmune hepatitis (AIH). We included a control group of subjects without liver damage. Tryptase-positive MCs were identified by indirect immunofluorescence and quantified as MC density per low-power field (MC/LPF). Group differences were analyzed using the Kruskal–Wallis test with Dunn’s multiple comparisons, considering p \u003C 0.05 as statistically significant. A significantly higher MC density was observed in MASLD, ALD, and AIH patients compared with the control group. The group analysis showed that ALD patients exhibited higher MC density than AIH, with no observed difference between ALD and MASLD. MC density was correlated positively with tobacco smoking and alcohol use in the full analyzed group, suggesting them as risk factors of high MC liver infiltration. We conclude that MC density is augmented in MASLD-related cirrhosis, highlighting potential links between lifestyle factors and MC-mediated hepatic inflammation. Future studies should explore the me","cbCaidKrcWb6n9fS","https://ap.wps.com/l/cbCaidKrcWb6n9fS","pdf",1725386,"English","# Abstract\n# 1. Introduction","[{\"question\":\"What was the study’s main aim regarding mast cells?\",\"answer\":\"To evaluate differences in mast cell (MC) density in cirrhotic liver tissue among patients with MASLD and other chronic liver disease etiologies.\"},{\"question\":\"How were mast cells identified and quantified?\",\"answer\":\"Tryptase-positive mast cells were identified by indirect immunofluorescence and quantified as MC density per low-power field (MC/LPF).\"},{\"question\":\"What clinical factors were associated with higher mast cell infiltration?\",\"answer\":\"MC density showed a positive correlation with tobacco smoking and alcohol use in the full analyzed group.\"}]","Increased Intrahepatic Mast Cell Density in Liver Cirrhosis - Due to MASLD and Other Non-Infectious Chronic Liver Diseases | PDF",1790765239,25]