[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-351066-105":59,"doc-detail-351066-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","incenp-and-cdca8-predict-neoadjuvant-chemotherapy-response-and-outcomes-in-esophageal-squamous-cell-carcinoma","INCENP and CDCA8 predict neoadjuvant chemotherapy response and outcomes in esophageal squamous cell carcinoma","","Neoadjuvant chemotherapy (NACT) improves prognosis but many patients fail due to treatment resistance, making predictive biomarkers essential. This study shows that elevated INCENP and CDCA8 expression is linked to poor NACT responsiveness across multiple cancers. INCENP 5′UTR and CDCA8 3′UTR undergo m6A methylation, are recognized by YTHDF3, and promote translation via eIF3A to drive resistance. Blocking INCENP and CDCA8 increases multipolar spindle formation and enhances NACT sensitivity, supporting biomarker and therapeutic targeting roles.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/incenp-and-cdca8-predict-neoadjuvant-chemotherapy-response-and-outcomes-in-esophageal-squamous-cell-carcinoma/351066/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/incenp-and-cdca8-predict-neoadjuvant-chemotherapy-response-and-outcomes-in-esophageal-squamous-cell-carcinoma/351066.png","ImageObject",300,407,{"name":92,"@type":93},"Asher","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-09-23","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":14},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main clinical problem addressed by this study?","Question",{"text":112,"@type":113},"Many patients develop resistance to neoadjuvant chemotherapy (NACT) and do not benefit from it, so reliable predictive biomarkers are urgently needed.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How do INCENP and CDCA8 relate to NACT resistance?",{"text":117,"@type":113},"Higher expression of INCENP and CDCA8 is associated with poor NACT responsiveness, and blocking them enhances NACT sensitivity.",{"name":119,"@type":110,"acceptedAnswer":120},"What molecular mechanism links m6A methylation to poor NACT response?",{"text":121,"@type":113},"INCENP and CDCA8 mRNAs are m6A-methylated and recognized by YTHDF3, which facilitates their translation through interaction with eIF3A, promoting resistance.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},351066,1790197046,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":14,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},687197207639,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Article [https://doi.org/10.1038/s41467-026-68371-x](https://doi.org/10.1038/s41467-026-68371-x)  \nINCENP and CDCA8 predict neoadjuvant chemotherapy response and outcomes in esophageal squamous cell carcinoma  \nReceived: 6 August 2024  \n\n| Accepted: 2 January 2026 |\n| --- |\n| |\n| Check for updates |\n\nXiangyu Wang1,2,7, Ting Wang1,2,7, Keke Wang2, Chengjuan Zhang3, Zhibo Li1,2, Feifei Liu1,2, Xueli Tian2,4, Xiaodan Shi1,2, Zihan Zhang1,2, Rui Wang1,2, Ludan Jia1,2, Kyle Vaughn Laster 2, Qingxin Xia3, Simin Zhao3,5,6  & Zigang Dong 1,2,3,5,6   \nNeoadjuvant chemotherapy (NACT), a key strategy for various cancers, markedly improves patient prognosis and 5-year survival rates. However, numerous patients develop resistance to NACT and thus fail to benefitfrom it. Therefore, identifying reliable biomarkers to predict patient responsiveness to NACT remains a critical challenge. Here, we demonstrate that elevated expression of INCENP and CDCA8 contributes to poor NACT responsiveness across multiple cancers. Mechanistically, the 5′UTR (GGACT at position 113) of INCENP and the 3′UTR (GGACT at position 1041) of CDCA8 undergo m⁶Amethylation and are recognized by YTHDF3, which facilitates their translation through interaction with eIF3A, ultimately driving poor response to NACT. Moreover, inhibition of INCENP and CDCA8 enhances NACT sensitivity by promoting multipolar spindle formation. Collectively, our findings establish that INCENP and CDCA8 serve as crucial biomarkers for predicting NACT responsiveness and as potential therapeutic targets for combination therapy with NACT to improve patient survival.  \nNeoadjuvant chemotherapy (NACT) has become a standard treatment for patients with various cancers undergoing surgery, particularly in lung cancer, breast cancer, ovarian cancer, and esophageal cancer1–4. It aims to reduce tumor size, improve surgical resectability, and lower the risk of postoperative recurrence. Among the commonly used NACT regimens, paclitaxel plus cisplatin is a primary first-line treatment for several malignancies5–7. Although this regimen is considered highly cytotoxic to tumors, its response rate in esophageal cancer remains limited at approximately 50–60%8,9. Notably, a subset of patients exhibits resistance to NACT, leading to tumor progression and significantly compromising both survival outcomes and quality of life10,11. Therefore, identifying reliable  \npredictive biomarkers for NACT sensitivity is essential for guiding personalized treatment.  \nThe mechanisms underlying resistance to NACT are complex, including tumor microenvironment influences and molecular heterogeneity of cancers12,13. Among these, dysregulation of the cell cycle has emerged as a key contributor. Mitosis is a fundamental biological process that regulates cell division and serves as a crucial therapeutic target in overcoming paclitaxel resistance14,15. Overexpression of mitotic regulators, such as cyclins and checkpoint proteins, can effectively halt or repair damage in mitosis, thereby diminishing the efficacy of paclitaxel16,17. Conversely, targeting these regulators, which leads to chromosomal instability or maintains multipolar spindles  \n1Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. 2China-US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. 3Department of Pathology, Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, Henan, China. 4Department of Clinical Research and Translational Medicine, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China. 5Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, Henan, China. 6The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan, China. 7These authors contributed equally: Xiangyu Wang, Ting Wang. e-mail: [smzhao@hci-cn.org](sm","cbCaijtngPOf58Tr","https://ap.wps.com/l/cbCaijtngPOf58Tr","pdf",4255244,18,"English","# Abstract\n## Background and challenge\n## Mechanistic findings\n## Therapeutic implications","[{\"question\":\"What is the main clinical problem addressed by this study?\",\"answer\":\"Many patients develop resistance to neoadjuvant chemotherapy (NACT) and do not benefit from it, so reliable predictive biomarkers are urgently needed.\"},{\"question\":\"How do INCENP and CDCA8 relate to NACT resistance?\",\"answer\":\"Higher expression of INCENP and CDCA8 is associated with poor NACT responsiveness, and blocking them enhances NACT sensitivity.\"},{\"question\":\"What molecular mechanism links m6A methylation to poor NACT response?\",\"answer\":\"INCENP and CDCA8 mRNAs are m6A-methylated and recognized by YTHDF3, which facilitates their translation through interaction with eIF3A, promoting resistance.\"}]","INCENP and CDCA8 predict neoadjuvant chemotherapy response and outcomes in esophageal squamous cell carcinoma | PDF",1790092464,45]