[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-333902-105":59,"doc-detail-333902-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","improving-tumor-microenvironment-assessment-in-chip-systems-through-next-generation-technology-integration","Improving tumor microenvironment assessment in chip systems through next-generation technology integration","","Tumor microenvironment (TME) assessment is central to understanding how diverse cancer and non-cancer cells drive cancer initiation, growth, invasion, therapy response, and drug resistance. While targeting the TME is promising, accurate reproduction of its complex cellular and noncellular interactions remains a challenge. Microfluidic systems better model key TME features than 2D co-culture or animal studies, enabling controlled study of dynamic multi-cell interactions. The review highlights integrated microfluidic approaches combining next-generation technologies such as bioprinting, single-cell sequencing, and real-time biosensing.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/improving-tumor-microenvironment-assessment-in-chip-systems-through-next-generation-technology-integration/333902/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/improving-tumor-microenvironment-assessment-in-chip-systems-through-next-generation-technology-integration/333902.png","ImageObject",300,407,{"name":92,"@type":93},"Riley","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-03","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the tumor microenvironment (TME), and why does it matter for cancer?","Question",{"text":112,"@type":113},"The TME includes diverse cancerous and non-cancerous cells such as stromal and immune cells. Their interactions influence tumor initiation, growth, invasion, therapy response, and drug resistance.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Why are microfluidic tumor-on-chip systems useful compared with 2D models and animal studies?",{"text":117,"@type":113},"Microfluidic systems more accurately mimic crucial TME features and allow precise, controlled examination of dynamic interactions among multiple human cell types over time.",{"name":119,"@type":110,"acceptedAnswer":120},"Which next-generation technologies are highlighted for improving TME assessment in chip systems?",{"text":121,"@type":113},"The review emphasizes integrated approaches combining bioprinting, single-cell sequencing, and real-time biosensing to dissect cellular intratumoral interactions across different tumor types.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},333902,1790197057,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},1374391975076,"https://ap-avatar.wpscdn.com/avatar/14000253ca4ec9f6853?x-image-process=image/resize,m_fixed,w_180,h_180&k=1783305029341752051","TYPE Review  \nPUBLISHED 25 September 2024 DOI 10.3389/fbioe.2024.1462293  \nOPEN ACCESS  \nEDITED BY  \nAlice Zoso,  \nPolytechnic University of Turin, Italy  \nREVIEWED BY  \nMonica Moya,  \nLawrence Livermore National Laboratory  \n(DOE), United States Giovanni Vozzi, University of Pisa, Italy  \n*CORRESPONDENCE  \nChristopher C. W. Hughes,  [cchughes@uci.edu](cchughes@uci.edu)  \nRECEIVED 09 July 2024  \nACCEPTED 10 September 2024  \nPUBLISHED 25 September 2024  \nCITATION  \nGaebler D, Hachey SJ and Hughes CCW (2024) Improving tumor microenvironment assessment in chip systems through nextgeneration technology integration.  \nFront. Bioeng. Biotechnol. 12:1462293 .  \ndoi: 10.3389/fbioe.2024.1462293  \nCOPYRIGHT  \n© 2024 Gaebler, Hachey and Hughes. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nImproving tumor microenvironment assessment inchip systems through  \nnext-generation technology integration  \nDaniela Gaebler 1, Stephanie J. Hachey 1 and Christopher C. W. Hughes 1,2*  \n1Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, United States, 2Biomedical Engineering, University of California, Irvine, Irvine, CA, United States  \nThe tumor microenvironment (TME) comprises a diverse array of cells, both cancerous and non-cancerous, including stromal cells and immune cells. Complex interactions among these cells play a central role in driving cancer progression, impacting critical aspects such as tumor initiation, growth, invasion, response to therapy, and the development of drug resistance. While targeting the TME has emerged as a promising therapeutic strategy, there is a critical need for innovative approaches that accurately replicate its complex cellular and noncellular interactions; the goal being to develop targeted, personalized therapies that can effectively elicit anti-cancer responses in patients. Microfluidic systems present notable advantages over conventional in vitro 2D co-culture models and in vivo animal models, as they more accurately mimic crucial features of the TMEand enable precise, controlled examination of the dynamic interactions among multiple human cell types at any time point. Combining these models with nextgeneration technologies, such as bioprinting, single cell sequencing and realtime biosensing, is a crucial next step in the advancement of microfluidic models. This review aims to emphasize the importance of this integrated approach to further our understanding of the TME by showcasing current microfluidic model systems that integrate next-generation technologies to dissect cellular intratumoral interactions across different tumor types. Carefully unraveling the complexity of the TME by leveraging next generation technologies will be pivotal for developing targeted therapies that can effectively enhance robust anti-tumoral responses in patients and address the limitations of current treatment modalities.  \nKEYWORDS  \ncancer immunology, tumor microenvironment, next-generation technology, tumor-onchip, bioengineering, biosensors, sequencing, bioprinting  \n1 Introduction  \nCancer is the second leading cause of death worldwide and cases are expected to exceed 2 million in the US alone in 2024 (Sung et al., 2021; Siegel et al., 2024), with the global cancer burden expected to further increase to 28.4 million cases in 2040 (Sung et al., 2021) . As a result, extensive efforts have been undertaken to deepen our understanding of the genetic  \nFrontiers in Bioengineering and Biotechnology 01 [frontiersin.org](frontiersin.org)  \nand molecular characteristics of this multifaceted disease. Cancer is d","cbCaivicgUyI4IAb","https://ap.wps.com/l/cbCaivicgUyI4IAb","pdf",2249474,27,"English","# Introduction\n## The tumor microenvironment (TME) and cancer progression\n## Rationale for microfluidic tumor-on-chip models\n## Integration of next-generation technologies","[{\"question\":\"What is the tumor microenvironment (TME), and why does it matter for cancer?\",\"answer\":\"The TME includes diverse cancerous and non-cancerous cells such as stromal and immune cells. Their interactions influence tumor initiation, growth, invasion, therapy response, and drug resistance.\"},{\"question\":\"Why are microfluidic tumor-on-chip systems useful compared with 2D models and animal studies?\",\"answer\":\"Microfluidic systems more accurately mimic crucial TME features and allow precise, controlled examination of dynamic interactions among multiple human cell types over time.\"},{\"question\":\"Which next-generation technologies are highlighted for improving TME assessment in chip systems?\",\"answer\":\"The review emphasizes integrated approaches combining bioprinting, single-cell sequencing, and real-time biosensing to dissect cellular intratumoral interactions across different tumor types.\"}]","Improving tumor microenvironment assessment in chip systems through next-generation technology integration | PDF",1790004540,68]