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This narrative review compiles methods to detect Ψ, describe its generation process, and assess how Ψ incorporation reshapes RNA metabolism and gene expression. It further analyzes how dysregulated Ψ and altered PUS activity contribute to cancer development, progression, and therapeutic responses, including mechanistic insights and biomarker relevance.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/implications-of-rna-pseudouridylation-for-cancer-biology-and-therapeutics-a-narrative-review/331362/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/implications-of-rna-pseudouridylation-for-cancer-biology-and-therapeutics-a-narrative-review/331362.png","ImageObject",300,407,{"name":92,"@type":93},"Riley","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is pseudouridine (Ψ) and why does it matter in cancer biology?","Question",{"text":112,"@type":113},"Ψ is a prevalent RNA modification formed from uridine, catalyzed by pseudouridine synthases (PUSs). The review links abnormal Ψ modification patterns to cancer-related dysregulation of RNA metabolism and function, affecting diagnosis, prognosis, and therapy responses.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the review evaluate methods for detecting Ψ?",{"text":117,"@type":113},"The review summarizes detection methods for Ψ and focuses on study designs with sufficient methodological detail and appropriate controls. It includes studies examining Ψ detection and specific Ψ/PUS mechanisms relevant to cancer.",{"name":119,"@type":110,"acceptedAnswer":120},"What role do PUSs play in cancer progression and potential treatment?",{"text":121,"@type":113},"Overexpression of PUSs is common in cancer cells and is associated with poor prognosis. The review reports that inhibiting PUSs can arrest cancer cell proliferation and enhance apoptosis, supporting PUS-targeting as a potential therapeutic strategy.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331362,1790168670,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374391975076,"https://ap-avatar.wpscdn.com/avatar/14000253ca4ec9f6853?x-image-process=image/resize,m_fixed,w_180,h_180&k=1783305029341752051","Ding et al. Journal of Translational Medicine (2024) 22:906  \n[https://doi.org/10.1186/s12967-024-05687-6](https://doi.org/10.1186/s12967-024-05687-6)  \nJournal of Translational Medicine  \nREVIEW Open Access  \nImplications of RNA pseudouridylation for cancer biology and therapeutics: a narrative review  \nHanyi Ding1,2, Na Liu3,5, Yan Wang1,3, Sofia Abdulkadir Adam3, Jie Jin1,3,4*, Weiying Feng2* and Jie Sun1,3,4*  \nAbstract  \nBackground Pseudouridine (Ψ), a C5-glycoside isomer of uridine, stands as one of the most prevalent RNA modifications in all RNA types. Distinguishing from the C-N bond linking uridine to ribose, the link between Ψ and ribose is a C-C bond, endowing Ψ modified RNA distinct properties and functions in various biological processes. The conversion of uridine to Ψ is governed by pseudouridine synthases (PUSs) . RNA pseudouridylation is implicated in cancer biology and therapeutics.  \nObjectives In this review, we will summarize the methods for detecting Ψ, the process of Ψ generation, the impact of Ψ modification on RNA metabolism and gene expression, the roles of dysregulated Ψ and pseudouridine synthases in cancers, and the underlying mechanism.  \nMethods We conducted a comprehensive search of PubMed from its inception through February 2024. The search terms included “pseudouridine”; “pseudouridine synthase”;“PUS”; “dyskerin”; “cancer”; “tumor”; “carcinoma”;“malignancy”; “tumorigenesis”; “biomarker”; “prognosis” and “therapy”. We included studies published in peer-reviewed journals that focused on Ψ detection, specific mechanisms involving Ψ and PUSs, and prognosis in cancer patients with high Ψ expression. We excluded studies lacking sufficient methodological details or appropriate controls.  \nResults Ψ has been recognized as a significant biomarker in cancer diagnosis and prognosis. Abnormal Ψ modifications mediated by various PUSs result in dysregulated RNA metabolism and impaired RNA function, promoting the development of various cancers. Overexpression of PUSs is common in cancer cells and predicts poor prognosis. PUSs inhibition arrests cell proliferation and enhances apoptosis in cancer cells, suggesting PUS-targeting cancer therapy may be a potential strategy in cancer treatment.  \nDiscussion High Ψ levels in serum, urine, and saliva may suggest cancer, but do not specify the type, requiring additional lab markers and imaging for accurate diagnosis. Standardized detection methods are also crucial for  \n*Correspondence:  \nJie Jin  \n[jiej0503@zju.edu.cn](jiej0503@zju.edu.cn)[ ](jiej0503@zju.edu.cn)Weiying Feng [fengweiying1997@126.com](fengweiying1997@126.com)[ ](fengweiying1997@126.com)Jie Sun [sunjiehm@zju.edu.cn](sunjiehm@zju.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/licenses/by-nc-nd/4.0/](creativecommons.org/licenses/by-nc-nd/4.0/.)[.](creativecommons.org/licenses/by-nc-nd/4.0/.)  \nDing et al. Journal of Translational Medicine (202","cbCaiaPySGiS2mka","https://ap.wps.com/l/cbCaiaPySGiS2mka","pdf",1216528,14,"English","# Abstract\n## Background\n## Objectives\n## Methods\n## Results\n## Discussion\n## Keywords\n# Introduction\n## Overview of RNA modifications\n## Detection and formation of pseudouridine (Ψ)","[{\"question\":\"What is pseudouridine (Ψ) and why does it matter in cancer biology?\",\"answer\":\"Ψ is a prevalent RNA modification formed from uridine, catalyzed by pseudouridine synthases (PUSs). The review links abnormal Ψ modification patterns to cancer-related dysregulation of RNA metabolism and function, affecting diagnosis, prognosis, and therapy responses.\"},{\"question\":\"How does the review evaluate methods for detecting Ψ?\",\"answer\":\"The review summarizes detection methods for Ψ and focuses on study designs with sufficient methodological detail and appropriate controls. It includes studies examining Ψ detection and specific Ψ/PUS mechanisms relevant to cancer.\"},{\"question\":\"What role do PUSs play in cancer progression and potential treatment?\",\"answer\":\"Overexpression of PUSs is common in cancer cells and is associated with poor prognosis. The review reports that inhibiting PUSs can arrest cancer cell proliferation and enhance apoptosis, supporting PUS-targeting as a potential therapeutic strategy.\"}]","Implications of RNA pseudouridylation for cancer biology and therapeutics - a narrative review | PDF",1789992917,35]