[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-327900-105":59,"doc-detail-327900-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","impacts-of-tissue-context-on-extracellular-vesicles-mediated-cancer-host-cell-communications","Impacts of tissue context on extracellular vesicles-mediated cancer - host cell communications","","Tumor tissue contains tightly packed cancer cells, non-cancerous cells, and extracellular matrix (ECM), forming functional structures that regulate cancer progression. Cancer cells deliver extracellular vesicles (EVs) to reprogram nearby normal cells into cancer-promoting states, shaping a favorable tumor microenvironment. Many EV studies rely on liquid-phase isolation or 2D culture, limiting insight into local EV transfer in vivo; this review summarizes current findings and analytical approaches to study EVs in physiological tissue contexts.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/impacts-of-tissue-context-on-extracellular-vesicles-mediated-cancer-host-cell-communications/327900/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/impacts-of-tissue-context-on-extracellular-vesicles-mediated-cancer-host-cell-communications/327900.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"Why is the tumor tissue context important for studying cancer extracellular vesicles (EVs)?","Question",{"text":113,"@type":114},"Tumor tissues include cancer and non-cancer host cells plus ECM, and these components collectively shape signaling and tumor-favorable tumor microenvironments. This context likely alters EV properties and the resulting EV-mediated cellular communications.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How do current EV research approaches limit understanding of EV transfer in tumors?",{"text":118,"@type":114},"Many studies analyze EVs isolated from liquid phases such as culture supernatants and blood, or use 2D cultured assays and systemic animal analyses. Such methods poorly capture local EV transfer and communication within physiological tumor structures.",{"name":120,"@type":111,"acceptedAnswer":121},"What factors and experimental directions are proposed to address remaining EV research questions in tumors?",{"text":122,"@type":114},"The review highlights that tissue environment may change EV properties and dynamics through physical barriers, interactions, and interstitial flows, and by creating variability in recipient cell types. It emphasizes physiological experimental settings and spatial analyses to study EV-mediated cancer–host cell interactions.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},327900,1790314708,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":41,"preview_page_count":4},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","DOI: 10. 1111/cas.16161  \nR E V I E W A RTI C LE  \nImpacts of tissue context on extracellular vesicles-mediated cancer–host cell communications  \nNao Nishida-Aoki1  | Takahiro Ochiya2   \n1Waseda Institute for Advanced Study, Waseda University, Tokyo, Japan 2Department of Molecular and Cellular Medicine, Center for Future Medical Research, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan  \nCorrespondence  \nNao Nishida-Aoki, Waseda Institute for Advanced Study, Waseda University, 1-21-1 Nishi Waseda, Shinjuku-ku, Tokyo 169-0051, Japan.  \nEmail: [nnishida@aoni.waseda.jp](nnishida@aoni.waseda.jp)  \nFunding information  \nNoguchi Institute; Japan Society for the Promotion of Science, Grant/Award Number: 23K06680 and 22K20805; Asfreya. Inc; Waseda University  \nAbstract  \nTumor tissue is densely packed with cancer cells, non-cancerous cells, and ECM, forming functional structures. Cancer cells transfer extracellular vesicles (EVs) to modify surrounding normal cells into cancer-promoting cells, establishing a tumor-favorable environment together with other signaling molecules and structural components. Such tissue environments largely affect cancer cell properties, and so as EV-mediated cellular communications within tumor tissue. However, current research on EVs focuses on functional analysis of vesicles isolated from the liquid phase, including cell culture supernatants and blood draws, 2D-cultured cell assays, or systemic analyses on animal models for biodistribution. Therefore, we have a limited understanding of local EV transfer within tumor tissues. In this review, we discuss the need to study EVsin a physiological tissue context by summarizing the current findings on the impacts of tumor tissue environment on cancer EV properties and transfer and the techniques required for the analysis. Tumor tissue environment is likely to alter EV properties, pose physical barriers, interactions, and interstitial flows for the dynamics, and introduce varieties in the cell types taken up. Utilizing physiological experimental settingsand spatial analyses, we need to tackle the remaining questions on physiological EVmediated cancer–host cell interactions. Understanding cancer EV-mediated cellular communications in physiological tumor tissues will lead to developing interactiontargeting therapies and provide insight into EV-mediated non-cancerous cells and interspecies interactions.  \nK E Y WO R D S  \ncellular spatial distribution, exosomes, extracellular vesicles, organotypic 3D culture models, tumor microenvironment  \nAbbreviations: BBB, blood-brain barrier; BRET, Bioluminescence Resonance Energy Transfer; DDS, drug delivery systems; ECM, extracellular matrix; EV, extracellular vesicle; HIFs, Hypoxia-inducible factors; HSPG, heparan sulfate proteoglycans; MMPs, metalloproteinase; MSC, mesenchymal stem cells; TME, tumor microenvironment; TNF-α , Tumor Necrosis Factor alpha; YAP, yes-associated protein.  \nThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.  \n© 2024 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.  \n1 | INTRODUCTION: WHY STUDYING CANCER EXTRACELLULAR VESICLES IN ATISSUE CONTEXT IS IMPORTANT?  \nTumor tissues consist of not only cancer cells but also non-cancerous host cells, such as fibroblasts, endothelial cells, and immune cells, together with structural support for the ECM. Within the tissues, cellular and non-cellular components interact with each other to develop a tumor-favorable TME.1 They transduce intercellular signals via direct contact and humoral factors such as growth factors, cytokines, metabolites, and EVs. EVs are nanosized lipid bilayer vesicles that are packed with cellular components such as DNA, RNA, proteins, and metabolites ","cbCaidiiAXh8tlYb","https://ap.wps.com/l/cbCaidiiAXh8tlYb","pdf",2270817,12,"English","# Abstract\n# Introduction: Why studying cancer extracellular vesicles in a tissue context is important?\n## Tumor microenvironment components and signaling pathways\n## Rationale for studying EVs within physiological tissue context","[{\"question\":\"Why is the tumor tissue context important for studying cancer extracellular vesicles (EVs)?\",\"answer\":\"Tumor tissues include cancer and non-cancer host cells plus ECM, and these components collectively shape signaling and tumor-favorable tumor microenvironments. This context likely alters EV properties and the resulting EV-mediated cellular communications.\"},{\"question\":\"How do current EV research approaches limit understanding of EV transfer in tumors?\",\"answer\":\"Many studies analyze EVs isolated from liquid phases such as culture supernatants and blood, or use 2D cultured assays and systemic animal analyses. Such methods poorly capture local EV transfer and communication within physiological tumor structures.\"},{\"question\":\"What factors and experimental directions are proposed to address remaining EV research questions in tumors?\",\"answer\":\"The review highlights that tissue environment may change EV properties and dynamics through physical barriers, interactions, and interstitial flows, and by creating variability in recipient cell types. It emphasizes physiological experimental settings and spatial analyses to study EV-mediated cancer–host cell interactions.\"}]","Impacts of tissue context on extracellular vesicles-mediated cancer - host cell communications | PDF",1789978296]