[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-331538-105":59,"doc-detail-331538-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","impact-of-cytotoxic-therapy-on-clonal-hematopoiesis-and-myeloid-neoplasms-in-breast-cancer-patients-observational-study","Impact of cytotoxic therapy on clonal hematopoiesis and myeloid neoplasms in breast cancer patients - Observational Study","","Clonal hematopoiesis (CH), characterized by mutated hematopoietic stem cells, is associated with an increased risk of subsequent myeloid neoplasms (MNs). This observational retrospective study evaluated CH prevalence and variant characteristics in breast cancer patients treated with cytotoxic therapy (CT), particularly among those who developed therapy-related myeloid neoplasms (MN-pCT). Peripheral blood and bone marrow were sequenced for CH in 107 patients at two time points, comparing change patterns across different CT exposures and assessing associations with MN-pCT development.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/impact-of-cytotoxic-therapy-on-clonal-hematopoiesis-and-myeloid-neoplasms-in-breast-cancer-patients-observational-study/331538/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/impact-of-cytotoxic-therapy-on-clonal-hematopoiesis-and-myeloid-neoplasms-in-breast-cancer-patients-observational-study/331538.png","ImageObject",300,407,{"name":92,"@type":93},"Seraphina","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the purpose of this study in breast cancer patients?","Question",{"text":112,"@type":113},"To investigate how cytotoxic therapy influences clonal hematopoiesis (CH) variants and how CH is associated with subsequent myeloid neoplasms after cytotoxic therapy (MN-pCT).","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were CH variants assessed and compared over time?",{"text":117,"@type":113},"The study retrospectively analyzed paired peripheral blood and bone marrow samples at two time points, tracking changes from before CT to after CT and comparing groups by CT exposure level.",{"name":119,"@type":110,"acceptedAnswer":120},"Does the presence of CH predict MN-pCT development in these patients?",{"text":121,"@type":113},"The results indicate that CH presence did not directly predict MN-pCT development, although CT induced measurable changes in CH genes.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},331538,1790168039,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":29,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962075114101,"https://ap-avatar.wpscdn.com/avatar/e000253a75eb197efd?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780044092746381165","®  \n Observational Study   \nImpact of cytotoxic therapy on clonal hematopoiesis and myeloid neoplasms in breast cancer patients  \nHeyjin Kim, MDa ,b ,c , Hyeon-Ok Jin, PhDc , Ji-Young Kim, MSc , Young Jun Hong, MD, PhDb ,c , and Jin Kyung Lee, MD, PhDb ,c ,*  \n\n| Abstract\u003Cbr>Clonal hematopoiesis (CH), which is characterized by variants of hematopoietic stem cells, increases the risk of subsequent myeloid neoplasms (MNs) . This study aimed to investigate the prevalence and characteristics of CH variants in breast cancer (BC) patients treated with cytotoxic therapy (CT), focusing on those who developed MNs after cytotoxic therapy (MN-pCT) . We retrospectively analyzed 107 BC patients from a biobank and sequenced peripheral blood and bone marrow samples from 31 CH-associated genes at 2 time points. We analyzed changes in CH for paired samples: T0 to T1 (before and after CT) and T1 to T2 (after CT vs greater CT exposure) . Additionally, we compared CH variants in patients with and without MN-pCT. 29% of patients harbored CH variants that were restricted to 8 genes and DNMT3A was the most frequent variant. Among 54 patients with paired samples (T1 to T2), the variant allele frequency (VAF) of CH variants significantly increased after greater CT exposure (P = .02) . However, there were no significant changes in VAF before and after CT. Five of the 9 patients who developed MN-pCT harbored CH variants. TP53 was the most frequently mutated gene, but it did not significantly affect MN-pCT risk compared to patients without CH variants. Although the presence of CH did not directly predict MN-pCT development in patients with BC, CT induced changes in CH genes. Further studies are required to determine the role of specific CH variants in the risk of MN-pCT and their potential as predictive biomarkers. |\n| --- |\n| Abbreviations: AML = acute myeloid leukemia, BC = breast cancer, C/RT = chemotherapy and/or radiotherapy, CH = clonal hematopoiesis, CT = cytotoxic therapy, MDS = myelodysplastic syndrome, MN = myeloid neoplasms, MN-pCT = myeloid neoplasms after cytotoxic therapy, NGS = next-generation sequencing, RR = relative risk, VAF = variant allele frequency. |\n| Keywords: breast cancer, clonal hematopoiesis, myeloid neoplasms post cytotoxic therapy, secondary hematological malignancy, therapy-related myeloid neoplasms |\n\n1. Introduction  \nCancer survivors face an elevated risk of developing second primary malignancies, including hematological ones.[1] This risk is influenced by the genetic background, environmental factors, and prior treatment history.[1,2] Clonal hematopoiesis (CH), which is characterized by mutated hematopoietic stem cells, plays a potential role in this increased risk.[3,4] Specifically, CH variants identified in cancer patients receiving cytotoxic therapy (CT) have been linked to a higher incidence of myeloid neoplasms after cytotoxic therapy (MN-pCT) .[3–5] These late-onset  \ncomplications include conditions such as myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), which have been termed therapy-related MDS and AML and are classified as distinct hematological malignancies.[6,7] Among the primary cancers, breast cancer (BC) is notably associated with the risk of MN-pCT.[2,8,9]  \nStudies suggest that up to 30% of cancer patients harbor CH variants in their blood, with a minimum variant allele frequency (VAF) of 2% .[2,4,10] However, recent large-scale investigations have revealed that CH prevalence increases with age and is associated with increased cardiovascular disease risk.[11–14] CH can  \nThis study was supported by the National Research Foundation of Korea (grant number NRF-2017M2A2A7A01070729) and Korea Institute of Radiological and Medical Sciences (grant numbers 50544-2022, 50574-2024), funded by the Ministry of Science and ICT, Republic of Korea.  \nThe authors have no conflicts of interest to disclose.  \nThe datasets generated during and/or analyzed during the current study are not publicly availa","cbCaiaQR2RhSMxA1","https://ap.wps.com/l/cbCaiaQR2RhSMxA1","pdf",622466,"English","# Introduction\n## Study support and disclosures\n## Methods overview and sequencing strategy","[{\"question\":\"What is the purpose of this study in breast cancer patients?\",\"answer\":\"To investigate how cytotoxic therapy influences clonal hematopoiesis (CH) variants and how CH is associated with subsequent myeloid neoplasms after cytotoxic therapy (MN-pCT).\"},{\"question\":\"How were CH variants assessed and compared over time?\",\"answer\":\"The study retrospectively analyzed paired peripheral blood and bone marrow samples at two time points, tracking changes from before CT to after CT and comparing groups by CT exposure level.\"},{\"question\":\"Does the presence of CH predict MN-pCT development in these patients?\",\"answer\":\"The results indicate that CH presence did not directly predict MN-pCT development, although CT induced measurable changes in CH genes.\"}]","Impact of cytotoxic therapy on clonal hematopoiesis and myeloid neoplasms in breast cancer patients - Observational Study | PDF",1789993799,15]