[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-509045-105":59,"doc-detail-509045-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","imaging-protocol-handbook-second-edition-cellular-analysis-step-by-step-imaging-protocols","Imaging Protocol Handbook - Second edition - Cellular analysis - Step-by-step imaging protocols","","Imaging protocol handbook for cellular analysis, presenting step-by-step instructions for common imaging workflows across cell proliferation, viability, structure, live-cell assays, immunolabeling, spatial biology and organoid labeling, and cell painting. It emphasizes EdU-based DNA synthesis for cell proliferation using the modified thymidine analog EdU, incorporated into newly synthesized DNA and labeled via a fast, photostable click reaction with Invitrogen Alexa Fluor dyes. It includes required materials, critical notes, and practical preparation steps, from stock solution setup through labeling, fixation, permeabilization, and continuation guidance.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/imaging-protocol-handbook-second-edition-cellular-analysis-step-by-step-imaging-protocols/509045/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/imaging-protocol-handbook-second-edition-cellular-analysis-step-by-step-imaging-protocols/509045.png","ImageObject",300,407,{"name":92,"@type":93},"Stanford","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-01","2026-10-05",true,{"@type":102,"interactionType":103,"userInteractionCount":29},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What does the EdU DNA synthesis-based cell proliferation assay measure?","Question",{"text":112,"@type":113},"It measures DNA synthesis by using EdU, a modified thymidine analog that is incorporated into newly synthesized DNA. The incorporated EdU is then fluorescently labeled for imaging.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What are the key steps after preparing the EdU reagents?",{"text":117,"@type":113},"Cells are labeled with the EdU-containing solution, followed by immediate fixation and permeabilization. After permeabilization, the protocol proceeds to the remaining imaging workflow steps.",{"name":119,"@type":110,"acceptedAnswer":120},"Which instruments and applications are referenced for using the protocol?",{"text":121,"@type":113},"The protocol notes use for detecting DNA synthesis with an EVOS imaging system. It also states it should not be used for flow cytometry and directs readers to a flow cytometry protocol instead.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},509045,1791758796,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":29,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336477552062,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","Cellular analysis  \nImaging protocol handbook Second edition of trusted imaging protocols  \nIntroduction  \nThis guide contains step-by-step instructions for common imaging protocols, including tips for a successful experiment, and lists of needed supplies. Invitrogen™ reagents are recognized all over the world as key components for publication-quality cellular images and analysis. We  \noffer cellular structure and functional probes, labels, and counterstains, as well as tools for achieving stunning cellular images. With the right preparation and a clear plan of action, you can obtain useful and beautiful cell images.  \nVisit [thermofisher.com/cellimaging](thermofisher.com/cellimaging to)[ to](thermofisher.com/cellimaging to) learn more.  \nContents  \nCell proliferation  \nClick-iT EdU Imaging Kits 1  \nClick-iT Plus EdU Imaging Kits protocol 5  \nClick-iT EdU Colorimetric IHC Detection Kit 9  \n Click-iT EdU labeling in vivo cell proliferation protocol 14 Cell viability  \nLIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells 21  \n ReadyProbes Cell Viability Imaging Kit, Blue/Red 22 Cell structure  \nMitoTracker mitochondrion-selective probes 23  \nPhalloidin reagents for actin labeling 26  \nCellLight BacMam 2.0 reagents 31  \nTubulin Tracker reagents 33  \nLysoTracker and LysoSensor probes 35  \nER-Tracker Dyes for live-cell endoplasmic reticulum labeling 37  \nSelectFX Nuclear Labeling Kit 40  \nStaining with Hoechst dyes 42  \nLabeling with SYTO 9 stain 44  \n Labeling with SYTO 59 stain 45 Live-cell assays  \nTetramethylrhodamine (TMRM) 47  \nCellEvent Caspase-3/7 Green Detection Reagent 49  \nCellROX Oxidative Stress Reagents 52  \nCellTracker Fluorescent Probes 53  \nImmunolabeling  \nICC formaldehyde fixed, permeabilized cells—direct with labeled primary antibodies 57  \nICC formaldehyde fixed, permeabilized cells—indirect with secondaries 59  \nICC biotin/streptavidin amplification 61  \nIHC indirect with secondaries on paraffin tissue 63  \nIHC indirect with secondaries on cryosection tissue 65  \nIHC biotin/streptavidin amplification on fixed tissue 67  \nSpatial biology and organoid labeling  \nStaining 3D cell cultures for imaging with antibodies and cellular reagents and dyes 69  \nMultiplexed IHC staining with primary antibodies conjugated to fluorophores 74  \nConjugating primary antibodies to fluorophores using ReadyLabel Antibody Labeling Kits 80  \nMultiplexed SuperBoost tissue staining with tyramides 86  \n ViewRNA tissue fluorescence assay protocol 96 Cell painting  \nCell painting 109  \nCell proliferation  \nClick-iT EdU Imaging Kits  \nDNA synthesis-based cell proliferation assay  \nIntroduction  \nIn this assay, the modified thymidine analog EdU (5-ethynyl-2'-deoxyuridine, is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Invitrogen™ Alexa Fluor™ dye in a fast, highly specific, mild click reaction.  \nMaterials  \n• Invitrogen™ Click-iT™ EdU Imaging Kit (Cat. No. C10338, C10340, C10337, C10339) . Refer to the user manual for component detail.  \n• PBS (Cat. No. 10010-023)  \n• Fixative (e.g. , Invitrogen Image-iT Fixation/Permeabilization Kit (Cat. No. R37602) or 4% formaldehyde in PBS (Cat. No. R37814))  \n• Permeabilization reagent (e.g. , Invitrogen™ Image-iT™ Fixation/Permeabilization Kit (Cat. No. R37602), or Thermo Scientific™ Triton™ X-100 (Cat. No. A16046 .AE))  \n• 3% bovine serum albumin (BSA) in PBS, pH 7.4  \n• Deionized water (Cat. No. 751-610)  \n• 18 × 18 mm coverslips  \n• Optional: 6-well microplate  \nCritical notes  \nThis protocol can be used for:  \n• Detecting DNA synthesis using an Invitrogen™ EVOS™ imaging system  \nThis protocol should not be used for:  \n• Flow cytometry; for a flow cytometry protocol, see Click-iT EdU Protocol for Flow Cytometry  \nProtocol  \nPrepare stock solutions  \n1. Allow vials to warm to room temperature.  \n\n| 2. Add 2 mL DMSO (component C) or an aqueous solution to component A to make a 10 mM EdU stock solution. Store at –20°C. |\n| --- |\n| 3. Ma","cbCaishGKhKtJIxE","https://ap.wps.com/l/cbCaishGKhKtJIxE","pdf",8966628,123,"English","# Introduction\n# Contents\n## Cell proliferation\n## Cell viability\n## Cell structure\n## Live-cell assays\n## Immunolabeling\n## Spatial biology and organoid labeling\n## Cell painting\n## DNA synthesis-based cell proliferation assay (Click-iT EdU)","[{\"question\":\"What does the EdU DNA synthesis-based cell proliferation assay measure?\",\"answer\":\"It measures DNA synthesis by using EdU, a modified thymidine analog that is incorporated into newly synthesized DNA. The incorporated EdU is then fluorescently labeled for imaging.\"},{\"question\":\"What are the key steps after preparing the EdU reagents?\",\"answer\":\"Cells are labeled with the EdU-containing solution, followed by immediate fixation and permeabilization. After permeabilization, the protocol proceeds to the remaining imaging workflow steps.\"},{\"question\":\"Which instruments and applications are referenced for using the protocol?\",\"answer\":\"The protocol notes use for detecting DNA synthesis with an EVOS imaging system. It also states it should not be used for flow cytometry and directs readers to a flow cytometry protocol instead.\"}]","Imaging Protocol Handbook - Second edition - Cellular analysis - Step-by-step imaging protocols | PDF",1791233969,310]