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ACC samples (16S rRNA sequencing) from a Huashan Hospital and China Medical University (HS) cohort were compared with The Cancer Genome Atlas (TCGA) datasets processed using Poore et al. and Salzberg et al. Alpha and beta diversity were not linked to survival, yet a five-genera ITB risk score trained in HS validated across both TCGA cohorts and independently predicted overall survival.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/identification-of-intratumor-bacteria-associated-prognostic-risk-score-in-adrenocortical-carcinoma-abstract/331970/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/identification-of-intratumor-bacteria-associated-prognostic-risk-score-in-adrenocortical-carcinoma-abstract/331970.png","ImageObject",300,407,{"name":92,"@type":93},"Olivia Brown","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What was the main outcome assessed in the study?","Question",{"text":113,"@type":114},"The primary outcome was overall survival (OS).","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How were intratumor bacteria (ITBs) profiles compared across datasets?",{"text":118,"@type":114},"The ITB profile from the HS cohort was compared to TCGA data processed by Poore et al. (TCGA-P) and by Salzberg et al. (TCGA-S).",{"name":120,"@type":111,"acceptedAnswer":121},"What prognostic model was developed and validated?",{"text":122,"@type":114},"A five-genera ITB risk score (Corynebacterium, Mycoplasma, Achromobacter, Anaerococcus, Streptococcus) trained in the HS cohort was validated in both TCGA-P and TCGA-S cohorts and was independently prognostic.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},331970,1790186440,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":41,"preview_page_count":4},16904993612988,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","| Host-Microbial Interactions | Research Article  \nIdentification of intratumor bacteria-associated prognostic risk score in adrenocortical carcinoma  \nLinyi Tan,1 Dengwei Zhang,2 Yong-xin Li,2 Yuqing Li,1 Ting Guo,3 Yang Sun,4 Ning Li,5 Chenchen Feng1  \nAUTHOR AFFILIATIONS See affiliation list on p. 10.  \nABSTRACT A landmark study by Poore et al. showed intratumor bacteria (ITBs) playing a critical role in most cancers by reproduction of The Cancer Genome Atlas (TCGA) transcriptome data. A recent study by Salzberg et al. argued that ITBs, being overstated as a methodology by Poore et al., were problematic. We previously reported that ITBs were prognostic in adrenocortical carcinoma (ACC), a highly aggressive rare disease using data by Poore et al., and here, we aimed to answer whether ITBs truly existed and were prognostic in ACC. ACC samples from our institutes underwent 16S rRNA sequencing [adrenocortical carcinoma blocks from Huashan Hospital and China Medical University (HS) cohort] . The ITB profile was compared to TCGA data processed by Pooreet al. (TCGA-P) and TCGA data processed by Salzberg et al. (TCGA-S), respectively. The primary outcome was overall survival (OS) . A total of 26 ACC cases (HS cohort) and 10 paraffin controls were sequenced. The TCGA cohort encompassed 77 cases. Two and four amid the top 10 abundant genera in HS cohort were not detected in TCGA-P and TCGA-S, respectively. Neither was alpha or beta diversity associated with survival nor could ACCbe subtyped by ITB signature in the HS cohort. Notably, a five-genera ITB risk score (Corynebacterium, Mycoplasma, Achromobacter, Anaerococcus, and Streptococcus) for OS trained in the HS cohort was validated in both TCGA-P and TCGA-S cohorts and was independently prognostic. Whereas ITB signature on the whole may not be associated with ACC subtypes, certain ITB features are associated with prognosis, and a risk score could be generated and validated externally.  \nIMPORTANCE In this report, we looked at the role of ITBs in ACC in patients with different race and sequencing platforms. We found a five-genera ITB risk score consistently predicted overall survival in all cohorts. We conclude that certain ITB features are universally pathogenic to ACC.  \nKEYWORDS adrenocortical carcinoma, intratumor bacteria, prognosis  \nA drenocortical carcinoma (ACC) is a rare disease with occult heralding symptoms.  \nThough curable at the localized stage, most patients are diagnosed at an advanced stage, which confers dismal survival. Identification of novel prognostic markers is urgently needed.  \nIn recent years, next-generation sequencing has brought surprise in cancer studies when scholars identified non-human reads that could be mapped to microbial signature (1) . Intratumor microorganisms, especially intratumor bacteria (ITBs), have gained the most attention, given their abundance in comparison to fungi, archaea, or virus. ITB signature has shown a prognostic effect in a variety of cancer types, including gastric cancer (2), colorectal cancer (3), and hepatocellular cancer (4), establishing the micro biome as a novel omics or “second genome” of cancer (5) .  \nUnlike gene-association studies, microbial signature in cancer is largely impacted by contamination, especially in organs conventionally regarded as “sterile” (6) . In breast  \nEditor Wei-Hua Chen, Huazhong University of Science and Technology, Wuhan, China  \nAddress correspondence to Chenchen Feng, [fengchenchen@fudan.edu.cn](fengchenchen@fudan.edu.cn), or Ning Li, [drlining_uro@163.com](drlining_uro@163.com).  \nLinyi Tan and Dengwei Zhang contributed equally to this article. Author order was determined based onther contribution to the article.  \nauthors declare conflict of interest  \ncancer, for instance, only one to two ITBs can be spotted in every ~10,000 cells scanned (7) . This makes identification of true ITBs in cancers with low biomass substantially challenging as a vast majority of microbial reads detected ","cbCaigl2pahRXfYX","https://ap.wps.com/l/cbCaigl2pahRXfYX","pdf",7072852,12,"English","# Materials and Methods\n## Sample collection","[{\"question\":\"What was the main outcome assessed in the study?\",\"answer\":\"The primary outcome was overall survival (OS).\"},{\"question\":\"How were intratumor bacteria (ITBs) profiles compared across datasets?\",\"answer\":\"The ITB profile from the HS cohort was compared to TCGA data processed by Poore et al. (TCGA-P) and by Salzberg et al. (TCGA-S).\"},{\"question\":\"What prognostic model was developed and validated?\",\"answer\":\"A five-genera ITB risk score (Corynebacterium, Mycoplasma, Achromobacter, Anaerococcus, Streptococcus) trained in the HS cohort was validated in both TCGA-P and TCGA-S cohorts and was independently prognostic.\"}]","Identification of Intratumor Bacteria-Associated Prognostic Risk Score in Adrenocortical Carcinoma - Abstract | PDF",1789996076]