[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-342627-105":59,"doc-detail-342627-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","identification-of-expression-profiles-and-prognostic-value-of-rfcs-in-colorectal-cancer","Identification of expression profiles and prognostic value of RFCs in colorectal cancer","","Colorectal cancer incidence and outcomes are linked to DNA damage, yet the prognostic impact of Replication Factor C (RFC) complexes remains unclear. Using computational resources including DepMap, GEPIA, DAVID/KEGG, Human Protein Atlas, STRING, and TIMER, the study finds RFC1 and RFC5 to be among the least expressed RFC subunits in CRC cell lines. Low RFC1/RFC5 expression associates with poorer overall survival, medium immunohistochemical staining, and links to miR-26a-5p and miR-636 affecting DNA repair and proliferation pathways, suggesting biomarker and therapeutic directions.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/identification-of-expression-profiles-and-prognostic-value-of-rfcs-in-colorectal-cancer/342627/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/identification-of-expression-profiles-and-prognostic-value-of-rfcs-in-colorectal-cancer/342627.png","ImageObject",300,407,{"name":92,"@type":93},"Lucas Martin","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Which RFC subunits show the lowest expression in colorectal cancer cell lines?","Question",{"text":112,"@type":113},"RFC1 and RFC5 display the least mRNA expression levels among RFC complex subunits in CRC cell lines compared with the others.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does low RFC1 and RFC5 expression relate to CRC patient prognosis?",{"text":117,"@type":113},"Low RFC1 and RFC5 expression correlates with poor prognosis, specifically worse overall survival in CRC patients.",{"name":119,"@type":110,"acceptedAnswer":120},"What microRNAs are linked to RFC1 and RFC5 expression and which pathways are implicated?",{"text":121,"@type":113},"Low RFC1 and RFC5 expression correlates with high miR-26a-5p and miR-636, influencing cell proliferation via mismatch repair, DNA replication, and nucleotide excision repair pathways.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},342627,1790181752,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},8796095360427,"https://ap-avatar.wpscdn.com/davatar_994ba38a5ba835b3df7d355c54d3ed8d","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nIdentification of expression profilesand prognostic value of RFCsin colorectal cancer  \nMd Misbah1,4,5*, Manoj Kumar2, Abul Kalam Najmi3 & Mymoona Akhtar4,6*  \nColorectal cancer (CRC) ranks among the most prevalent cancers globally, with its incidence closely tied to DNA damage. The Replication Factor C (RFC) complexes comprises five protein subunits:  \nRFC1, RFC2, RFC3, RFC4, and RFC5. These RFC complexes play crucial roles in DNA replication, repair pathways, activities post DNA damage, and ATP-dependent processes during DNA synthesis. However, the impact of RFC complexes proteins on CRC prognosis remains unclear. To explore this, we employed a computational analysis approach, utilizing platforms such as the DepMap portal, GEPIA, DAVID Bioinformatics for KEGG pathway analysis, Human Protein Atlas (HPA), STRING, and  \nTIMER. Our results indicate that the mRNA levels of RFC1 and RFC5 were the least expressed among CRC cell lines compared to other RFC complex subunits. Notably, low RFC1 and RFC5 expression was correlated with poor prognosis in terms ofCRC patients’ overall survival (OS) . Immunohistochemical results from the Human Protein Atlas demonstrated medium staining for RFC1, RFC2, and RFC5 in CRC tissues. Furthermore, the low expression of RFC1 and RFC5 showed a significant correlation with high expression levels of miR-26a-5p and miR-636, impacting cell proliferation through mismatch repair, DNA replication, and the nucleotide excision repair pathway. Although the precise functions of RFC1 in cancer are still unknown, our findings suggest that the small-molecule single target, CHEMBL430483, and multiple target molecules could be potential treatments for CRC. In conclusion, the elevated expression of miR-26a-5p and miR-636 targeting RFC1 and RFC5 expression holds promise as a potential biomarker for early-stage CRC detection. These insights provide novel directions and strategies for CRC therapies.  \nAbbreviations  \nRFCs Replication factor CCRC Colorectal cancer  \nCCLE Cancer cell line encyclopedia  \nCOAD Colon adenocarcinoma READ Rectal adenocarcinoma  \nGEPIA Gene expression profiling interactive analysis  \nTIMER Tumor immune estimation resources OS Overall survival  \nSTRING Search tool for the retrieval of interacting genes/proteins  \nColorectal cancer (CRC) is the world’s third most common epithelial malignancy. According to the GLOBOCON database, around 147,950 individuals were diagnosed with CRC, and 53,200 patients succumbed to CRC in the United States in 20201. Surgery is the primary intervention for CRC patients in the early stage of diagnosis; however, it proves ineffective in metastatic cases. About 20% of patients exhibit micro-metastasis or metastatic (m)CRC post-surgery2,3. Biomarkers like mitogen-activated protein kinase 1 (MAPK1), phosphatidylinositol 3-kinase (PI3K), and others are presently employed for CRC treatment4,5. An increasing number of biomarkers  \n1International Ph. D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. 2Centre for Translational and Clinical Research, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, India. 3Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India. 4Bioinformatics Infrastructure Facility, Jamia Hamdard, New Delhi, India. 5Kusumraj Institute of Pharmacy, Bikram, Patna, Bihar, India 801104. 6Drug Design and Medicinal Chemistry Lab, Department of Pharmaceutical Chemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.* email: [d142109019@tmu.edu.tw](d142109019@tmu.edu.tw); [makhtar@jamiahamdard.ac.in](makhtar@jamiahamdard.ac.in)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \nare being utilized for the diagnosis and selection of therapy for CRC patients6. Hence, identifying valuable biomarkers for patient identifi","cbCaimaEEmXSS33Y","https://ap.wps.com/l/cbCaimaEEmXSS33Y","pdf",4057730,17,"English","# Results\n## Expression level of RFCs in CRC using CCLE and GEPIA2","[{\"question\":\"Which RFC subunits show the lowest expression in colorectal cancer cell lines?\",\"answer\":\"RFC1 and RFC5 display the least mRNA expression levels among RFC complex subunits in CRC cell lines compared with the others.\"},{\"question\":\"How does low RFC1 and RFC5 expression relate to CRC patient prognosis?\",\"answer\":\"Low RFC1 and RFC5 expression correlates with poor prognosis, specifically worse overall survival in CRC patients.\"},{\"question\":\"What microRNAs are linked to RFC1 and RFC5 expression and which pathways are implicated?\",\"answer\":\"Low RFC1 and RFC5 expression correlates with high miR-26a-5p and miR-636, influencing cell proliferation via mismatch repair, DNA replication, and nucleotide excision repair pathways.\"}]","Identification of expression profiles and prognostic value of RFCs in colorectal cancer | PDF",1790047452,43]