[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-32095":3,"doc-seo-32095":28},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"file_id":15,"file_url":16,"file_type":17,"file_size":18,"view_count":19,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"table_of_contents":24,"faqs":25,"seo_title":13,"seo_description":14,"update_tm":26,"read_time":27},32095,16904993612988,"Olivia Brown","https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd",8,"Research & Report","Identification of Eimeria acervulina in Broiler Chickens Using Morphology and Molecular Analyses","Coccidiosis, an intestinal protozoan disease affecting poultry worldwide, varies in severity with the identity of the causative agents. This study identified Eimeria species infecting broiler chickens (Gallus gallus domesticus) by combining morphological assessment with molecular phylogenetic analyses. Twenty-five faecal samples from a commercial farm in Riyadh were examined using floatation microscopy and DNA amplification of 18S rRNA, ITS-1, and COI genes. Eight of 25 samples were coccidiosis-positive, with oocyst morphology consistent with Eimeria acervulina and molecular clustering confirming this identification, supporting molecular diagnostics for long-term control.","cbCaiodttk0Qyve6","https://ap.wps.com/l/cbCaiodttk0Qyve6","pdf",2015708,7,1,10,"English","en","# Abstract\n# Introduction\n## Background: coccidiosis and Eimeria lifecycle\n## Eimeria species diversity and pathogenicity\n## Traditional morphology-based identification and limitations\n## PCR-based molecular approaches for avian Eimeria","[{\"question\":\"What was the main objective of the study?\",\"answer\":\"To identify the Eimeria species infecting broiler chickens by integrating morphological criteria with molecular phylogenetic analyses.\"},{\"question\":\"How were the samples collected and screened for Eimeria?\",\"answer\":\"Twenty-five faecal samples were collected from broilers at a commercial poultry farm in Riyadh and examined microscopically using the floatation technique.\"},{\"question\":\"Which molecular markers were used for identification and confirmation of Eimeria acervulina?\",\"answer\":\"DNA amplification and sequence clustering were based on partial 18S rRNA, ITS-1, and mitochondrial COI genes, which confirmed the morphological identification of Eimeria acervulina.\"}]",1780866095,25,{"code":4,"msg":29,"data":30},"ok",{"site_id":31,"language":23,"slug":32,"title":13,"keywords":33,"description":14,"schema_data":34,"social_meta":86,"head_meta":88,"extra_data":90,"updated_unix":26},105,"identification-of-eimeria-acervulina-in-broiler-chickens-using-morphology-and-molecular-analyses","",{"@graph":35,"@context":85},[36,53,68],{"@type":37,"itemListElement":38},"BreadcrumbList",[39,43,47,50],{"item":40,"name":41,"@type":42,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":44,"name":45,"@type":42,"position":46},"https://docshare.wps.com/document/","Document",2,{"item":48,"name":12,"@type":42,"position":49},"https://docshare.wps.com/document/research-report/",3,{"item":51,"name":13,"@type":42,"position":52},"https://docshare.wps.com/document/identification-of-eimeria-acervulina-in-broiler-chickens-using-morphology-and-molecular-analyses/32095/",4,{"url":51,"name":13,"@type":54,"author":55,"headline":13,"publisher":57,"fileFormat":60,"description":14,"dateModified":61,"datePublished":62,"encodingFormat":60,"isAccessibleForFree":63,"interactionStatistic":64},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":40,"name":58,"@type":59},"DocShare","Organization","application/pdf","2026-06-12","2026-06-07",true,{"@type":65,"interactionType":66,"userInteractionCount":19},"InteractionCounter",{"@type":67},"ViewAction",{"@type":69,"mainEntity":70},"FAQPage",[71,77,81],{"name":72,"@type":73,"acceptedAnswer":74},"What was the main objective of the study?","Question",{"text":75,"@type":76},"To identify the Eimeria species infecting broiler chickens by integrating morphological criteria with molecular phylogenetic analyses.","Answer",{"name":78,"@type":73,"acceptedAnswer":79},"How were the samples collected and screened for Eimeria?",{"text":80,"@type":76},"Twenty-five faecal samples were collected from broilers at a commercial poultry farm in Riyadh and examined microscopically using the floatation technique.",{"name":82,"@type":73,"acceptedAnswer":83},"Which molecular markers were used for identification and confirmation of Eimeria acervulina?",{"text":84,"@type":76},"DNA amplification and sequence clustering were based on partial 18S rRNA, ITS-1, and mitochondrial COI genes, which confirmed the morphological identification of Eimeria acervulina.","https://schema.org",{"og:url":51,"og:type":87,"og:title":13,"og:site_name":58,"og:description":14},"article",{"robots":89,"canonical":51},"index,follow",{"doc_id":7,"site_id":31}]