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Investigating the potential function and clinical significance of miRNA-mediated mRNA regulatory networks in liver cancer can offer novel insights and therapeutic strategies for the treatment of this disease. The study identifies 300 differentially expressed miRNAs, with five miRNAs linked to overall survival and functioning as independent prognostic markers. GO and KEGG enrichment highlight pathways including carboxylic/organic acid biosynthesis and liver cancer-related metabolism, while hub gene analysis implicates prognosis-associated targets such as ABAT, BHMT, and SHMT1 regulated by miR-5003-3p.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/identification-and-validation-of-key-mirnas-and-a-microrna-mrna-regulatory-network-associated-with-liver-cancer-research-paper/332154/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/identification-and-validation-of-key-mirnas-and-a-microrna-mrna-regulatory-network-associated-with-liver-cancer-research-paper/332154.png","ImageObject",300,407,{"name":92,"@type":93},"Hazel","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":24},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main aim of the study on liver cancer miRNAs?","Question",{"text":112,"@type":113},"The study aims to identify and validate key miRNAs and map a miRNA-mRNA regulatory network linked to liver cancer, including potential clinical significance and therapeutic implications.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How many differentially expressed miRNAs were identified, and what prognostic value was found?",{"text":117,"@type":113},"The study identified 300 differentially expressed miRNAs. Five miRNAs were associated with overall survival and could serve as independent prognostic biomarkers.",{"name":119,"@type":110,"acceptedAnswer":120},"Which miRNA is highlighted as regulating genes involved in liver cancer development?",{"text":121,"@type":113},"miR-5003-3p is highlighted as inhibiting ABAT, BHMT, and SHMT1 expression, thereby promoting liver cancer development.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},332154,1790014832,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":24,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":36,"preview_page_count":4},137441390410,"https://ap-avatar.wpscdn.com/avatar/2000252f4ab5702993?_k=1776741390130283984","CELL CYCLE  \n2024, VOL. 23, NO. 4, 353–368  \n[https://doi.org/10.1080/15384101.2024.2335024](https://doi.org/10.1080/15384101.2024.2335024)  \nRESEARCH PAPER     \nIdentification and validation of key miRNAs and a microRNA-mRNA regulatory network associated with liver cancer  \nJie Tanga,b*, Song Lic*, Zixiao Zhoud, Weicai Changb, Yongqiang Wanga, Juan Meie, and Shaobo Zhoua  \naGeneral Surgery, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China; bAnhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu, China; cDepartment of Hepatobiliary pancreatic gastrointestinal Surgery, JinHua People’s Hospital, JinHua, China; dXiangya Medical College, Central South University, Changsha, China; ePathology Department, The Second Affiliated Hospital of Bengbu Medical College, Bengbu, China  \nABSTRACT  \nMiRNAs play crucial regulatory roles in the growth and development of tumor cells by serving as carriers of post-transcriptional regulatory information derived from genes. Investigating the potential function and clinical significance of miRNA-mediated mRNA regulatory networks in liver cancer can offer novel insights and therapeutic strategies for the treatment of this disease. We identified 300 differentially expressed miRNAs, and five miRNAs were identified to be correlated with overall survival and could be used as an independent prognostic. GO enrichment analysis mainly included carboxylic acid biosynthesis, organic acid biosynthesis, peroxisomal membrane, microsomal membrane, DNA binding, C-acyltransferase activity, etc. KEGG enrichment analysis showed that the pathways of target genes related to liver cancer were mainly focused on butyric acid metabolism and partial amino acid metabolism. Eight of the top 10 HUB genes were associated with prognosis, and the expression of four genes was positively correlated with prognosis, of which ABAT, BHMT, and SHMT1 were target genes of hsa-miR-5003-3p. MiR-5003- 3p inhibits ABAT/BHMT/SHMT1 expression, thereby promoting liver cancer development. Overall, our study provides new ideas for the treatment of liver cancer, and these five miRNAs may be independent prognostic biomarkers and therapeutic targets for liver cancer patients. And miR- 5003-3p may be a critical factor in the mechanism of liver cancer development.  \nARTICLE HISTORY  \nReceived 16 January 2024 Revised 26 February 2024 Accepted 11 March 2024  \nKEYWORDS  \nLiver cancer; miRNA; mRNA; prognostic biomarker; miR-5003-3p  \n1. Introduction  \nGlobally, liver cancer is a common cause of cancer-related death, and its major risk factors include viral hepatitis and excessive alcohol consumption [ 1–3] . Due to the high degree of malignancy and poor prognosis of liver cancer, there are mostly no specific clinical symptoms in the early stages. Most of them are already in the middle or late stages when detected, at which time the effectiveness of treatment options is significantly reduced. The survival and prognosis of the patients become the focus of attention [4,5] . So far, the molecular mechanism of liver cancer is still unclear, and the incidence rate continues to rise [6,7], which requires researchers to continuously explore and find biomarkers for early diagnosis and prognosis with higher accuracy.  \nMicroRNAs (miRNAs) are short non-coding RNAs of approximately 22 nucleotides in length [8], which serve as carriers of post-transcriptional regulatory information encoded by genes and have important regulatory roles in the growth and development of numerous cancers [9–11] . For example, miR-155 is lowly expressed in liver cancer cells and inhibits the proliferation and metastasis of cancer cells [ 12]; MIR-373 inhibits autophagy and promotes apoptosis of cholangiocarcinoma cells by targeting ULK1 [ 13]; and miR- 4733-5p is up-regulated in gallbladder cancer and promotes the proliferation of gallbladder cancer cells by directly binding to KLF [ 14] . This shows that miRNAs may be key factors in regulating onc","cbCaih0mL1vcEbTf","https://ap.wps.com/l/cbCaih0mL1vcEbTf","pdf",12823210,16,"English","# Introduction\n## MicroRNAs and cancer relevance\n# Materials and methods\n## Acquisition and processing of data\n# Results and analysis (described in abstract)","[{\"question\":\"What is the main aim of the study on liver cancer miRNAs?\",\"answer\":\"The study aims to identify and validate key miRNAs and map a miRNA-mRNA regulatory network linked to liver cancer, including potential clinical significance and therapeutic implications.\"},{\"question\":\"How many differentially expressed miRNAs were identified, and what prognostic value was found?\",\"answer\":\"The study identified 300 differentially expressed miRNAs. Five miRNAs were associated with overall survival and could serve as independent prognostic biomarkers.\"},{\"question\":\"Which miRNA is highlighted as regulating genes involved in liver cancer development?\",\"answer\":\"miR-5003-3p is highlighted as inhibiting ABAT, BHMT, and SHMT1 expression, thereby promoting liver cancer development.\"}]","Identification and validation of key miRNAs and a microRNA-mRNA regulatory network associated with liver cancer - Research Paper | PDF",1789997082]