[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-332529-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-332529-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","hyperplastic-ovarian-stromal-cells-express-genes-associated-to-tumor-progression-a-case-study","Hyperplastic ovarian stromal cells express genes associated to tumor progression - a case study","","Study analyzes stromal cells derived from a hyperplastic left ovary of a Holstein cow, cultured until the 8th passage and characterized by fibroblast-like morphology with halted proliferation. Non-cancerous stromal identity is supported by in vitro proliferation and migration assays, while spent media show negligible estradiol (E2) consistent with non-estradiol-synthesizing cells. Immunofluorescence and gene expression reveal vimentin expression, and histology shows a dense stromal cell population. Genome-wide mRNA microarrays detect 1396 differential genes with key upregulation of ASPN and VCAM1. Ingenuity pathway analysis indicates activation of “Sirutin Signaling Pathway” and “Mitochondrial Dysfunction” with inhibition of “Oxidative phosphorylation,” and S-phase enrichment links hyperplasia to proliferation genes CCND2 and CDK6.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/hyperplastic-ovarian-stromal-cells-express-genes-associated-to-tumor-progression-a-case-study/332529/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/hyperplastic-ovarian-stromal-cells-express-genes-associated-to-tumor-progression-a-case-study/332529.png","ImageObject",300,407,{"name":42,"@type":43},"Riley","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-29","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",11,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What type of tissue and cells were studied in this case report?","Question",{"text":63,"@type":64},"Stromal cells were isolated from a hyperplastic left ovary of a Holstein cow and then cultured for experimental analyses.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did the study assess whether the stromal cells were non-cancerous?",{"text":68,"@type":64},"In vitro proliferation and migration assays, along with negligible estradiol (E2) detected in spent media, supported a non-cancerous, non-estradiol-synthesizing stromal phenotype.",{"name":70,"@type":61,"acceptedAnswer":71},"What genetic and pathway changes were identified in hyperplastic stromal cells?",{"text":72,"@type":64},"mRNA microarray analysis found 1396 differentially expressed genes, including strong upregulation of ASPN and VCAM1. Ingenuity pathway analysis highlighted activation of specific pathways such as “Sirutin Signaling Pathway” and “Mitochondrial Dysfunction,” while “Oxidative phosphorylation” was inhibited.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},332529,1790655925,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":128,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},1374391975076,"https://ap-avatar.wpscdn.com/avatar/14000253ca4ec9f6853?x-image-process=image/resize,m_fixed,w_180,h_180&k=1783305029341752051","Sharma et al. BMC Veterinary Research (2024) 20:439  \n[https://doi.org/10.1186/s12917-024-04275-6](https://doi.org/10.1186/s12917-024-04275-6)  \nBMC Veterinary Research  \nRESEARCH Open Access  \nHyperplastic ovarian stromal cells express genes associated to tumor progression: a case study  \nArpna Sharma 1,3*, Frank Becker 1, Xuelian Tao 1, Vijay Simha Baddela 1, Dirk Koczan2, Carol in Ludwig 1 and Jens Vanselow 1  \nAbstract  \nThe current study presents the analysis of stromal cells obtained from an hyperplastic left-ovary of a Holstein cow. Cultured hyperplastic stromal cells displayed a fibroblast-like morphology and ceased proliferation after the 8th passage. The non-cancerous nature of stromal cells was confirmed by in vitro cell proliferation and migration assays. Negligible amounts of E2 were detected in the spent media of cultured stromal cells, which suggests that stromal cells were non-estradiol synthesizing cells. As revealed in immunofluorescence and gene expression analysis, the hyperplastic stromal cells explicitly expressed vimentin in their cytoskeleton. Upon hematoxylin staining, a highly dense population of stromal cells was observed in the stromal tissue of the hyperplastic ovary. To explore genome-wide alterations, mRNA microarray analysis was performed using Affymetrix Bovine Gene 1. 0STArrays compared to normal ovarian derived stromal cells. The microarray identified 1396 differential ly expressed genes, of which 733 were up-and 663 down-regulated in hyperplastic stromal cells. Importantly, asporin (ASPN) and vascular cell adhesion molecule 1 (VCAM1) were among the highly up-regulated genes. Higher expression of ASPN was also confirmed by immunohistochemistry and RT-qPCR analysis. Ingenuity pathway analysis (IPA) identified about 98 significantly enriched (-log (p value ≥ 1. 3) canonical pathways, importantly of which the “Sirutin Signaling Pathway” and “Mitochondrial Dysfunction” were highly activated while “Oxidative phosphorylation”was inhibited. Additionally, higher proportion of hyperplastic stromal cells in the S-phase of cell cycle, could be attributed to higher expression levels of cell proliferation genes such as CCND2 and CDK6 .  \nKeywords Ovary, Stroma, Hyperplastic  \nIntroduction  \nThe bovine ovary consists primarily of two types of tissues such as (1) specialized parenchymal tissue enclosing numerous growing follicles containing oocytes and  \n*Correspondence:  \nArpna Sharma  \n[sharma@fbn-dummerstorf.de](sharma@fbn-dummerstorf.de)  \n1Forschungsinstitut für Nutztierbiologie (FBN), Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany  \n2Institut für Immunologie, Universität Rostock, 18055 Rostock, Germany 3Department of Animal Sciences, University of Illinois UrbanaChampaign, Urbana, USA  \n(2) supportive connective tissue, commonly referred as ovarian stroma. The ovarian stroma consists of a heterogeneous population of cells along with incompletely characterized stromal cells such as fibroblast-like spindle cells and interstitial cells [1]. In the ovarian stroma, fibroblastlike stromal cells are arranged in a characteristic whorled texture [2]. There has been a great interest in identifying and understanding the functions of these incompletely characterized stromal cells that largely produce collagen, a vital part of the extracellular matrix responsible for overall cell support, adhesion, and motility [3–6]. Non-neoplastic proliferation of stromal cells in the ovary can result into  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a ","cbCaikakM1NorG9f","https://ap.wps.com/l/cbCaikakM1NorG9f","pdf",8031486,"English","# Abstract\n# Keywords\n# Introduction\n## Ovarian tissue composition and stromal hyperplasia background\n## Rarity of cattle cases and motivation for the present study\n# Case description","[{\"question\":\"What type of tissue and cells were studied in this case report?\",\"answer\":\"Stromal cells were isolated from a hyperplastic left ovary of a Holstein cow and then cultured for experimental analyses.\"},{\"question\":\"How did the study assess whether the stromal cells were non-cancerous?\",\"answer\":\"In vitro proliferation and migration assays, along with negligible estradiol (E2) detected in spent media, supported a non-cancerous, non-estradiol-synthesizing stromal phenotype.\"},{\"question\":\"What genetic and pathway changes were identified in hyperplastic stromal cells?\",\"answer\":\"mRNA microarray analysis found 1396 differentially expressed genes, including strong upregulation of ASPN and VCAM1. Ingenuity pathway analysis highlighted activation of specific pathways such as “Sirutin Signaling Pathway” and “Mitochondrial Dysfunction,” while “Oxidative phosphorylation” was inhibited.\"}]","Hyperplastic ovarian stromal cells express genes associated to tumor progression - a case study | PDF",1789998613,48]