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The document provides intended use, storage and stability guidance, biosafety caution, and a step-by-step protocol for initiating and maintaining stock cultures in Medium 254 with HMGS-2.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/human-epidermal-melanocytes-adult-hema-lp-product-catalog-and-protocol/640592/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/human-epidermal-melanocytes-adult-hema-lp-product-catalog-and-protocol/640592.png","ImageObject",300,407,{"name":42,"@type":43},"Franzy","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-10","2026-10-22",true,{"@type":52,"interactionType":53,"userInteractionCount":30},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What does each vial of HEMa-LP contain and how are the cells prepared?","Question",{"text":62,"@type":63},"Each vial contains at least 5 × 105 viable HEMa-LP cells cryopreserved after the secondary culture stage in a DMSO-containing medium. Cells are thawed for use in culture work.","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"How are contamination and specific agents checked before the product is released?",{"text":67,"@type":63},"An independent laboratory tests for mycoplasma, Hepatitis B, Hepatitis C, and HIV-1, and those agents were not detected. The product is also performance tested by culturing through multiple passages and verifying no bacterial, yeast, or fungal contamination during the culture period.",{"name":69,"@type":60,"acceptedAnswer":70},"What are the key recommendations for storage and handling after shipping?",{"text":71,"@type":63},"HEMa-LP should arrive frozen on dry ice and be placed immediately into liquid nitrogen vapor phase storage. 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No. MAN0001603 Rev. 3.0  \nProduct description  \nHEMa-LP are human epidermal melanocytes isolated from lightly pigmented (LP) adult skin. Each vial of this product contains ≥5 × 105 viable cells that have been cryopreserved at the end of the secondary culture stage in a medium containing dimethylsulfoxide (DMSO) . The cells in each lot stain positively with Mel-5 antibody1,2 in the fourth culture after thawing. An independent laboratory tests the cells for the presence of mycoplasma, Hepatitis B, Hepatitis C, and HIV-1 viruses. These agents were not detected. In our laboratory, each lot of cells is performance tested by culturing the cells through multiple passages in Medium 254 supplemented with Human Melanocyte Growth Supplement-2 (HMGS-2, Cat. No. S-016-5) in the absence of antibiotics and antimycotics. During this culture period, no contamination by bacteria, yeast, or fungi was detected. Upon thawing, the cells are guaranteed to be ≥70% viable and to have a potential of ≥16 population doublings when handled according to the directions provided in this document. For recommended precautions for handling human cells, read the Caution statement.  \n\n| Product | Catalog No. | Amount | Shipping | Storage |\n| --- | --- | --- | --- | --- |\n| Human Epidermal Melanocytes, adult (HEMa-LP) | C-024-5C | 1 vial\u003Cbr>(≥5 × 105 viable cells/vial) | Frozen on dry ice | Liquid nitrogen vapor phase |\n\nIntended use  \nCryopreserved HEMa-LP are intended for use by researchers investigating the molecular and biochemical basis of various normal and disease processes. This product is for research use only. Not for use in animals, humans, or diagnostic procedures.  \nStorage and stability  \nCryopreserved HEMa-LP should arrive frozen on dry ice. If the cells are not to be used immediately, prepare a space for storage of the vialin the vapor phase of a liquid nitrogen freezer. While wearing protective eyewear, gloves, and a laboratory coat, remove the vial from its shipping container and place immediately in the liquid nitrogen freezer. Although the viability of cryopreserved cells decreases with time in storage, useful cultures can usually be established even after 2 years of storage at liquid nitrogen temperatures.  \nCaution  \nAlthough cryopreserved cells have been tested for the presence of various hazardous agents, diagnostic tests are not necessarily 100% accurate. In addition, human cells may harbor other known or unknown agents, or organisms which could be harmful to your health or cause fatal illness. Treat all human cells as potential pathogens. Wear protective clothing and eyewear. Practice appropriate disposal techniques for potentially pathogenic or biohazardous materials. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.  \nFor Research Use Only. Not for use in diagnostic procedures.  \nInitiate cultures from cryopreserved cells  \nWe recommend seeding cells recovered from cryopreservation at a density of 5 × 103 viable cells/cm2. For example, four 25-cm2 tissue culture flasks can usually be established from one vial containing ≥5 × 105 HEMa-LP. The following procedure is a sample protocol for establishing cultures from the contents of one vial.  \n1. Prepare a bottle of supplemented Medium 254 (Cat. No. M-254-500) according to the instructions supplied with that product. Note: Although Medium 254 can be supplemented with PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2, Cat. No.  \nS-016-5) or with traditional Human Melanocyte Growth Supplement (HMGS, Cat. No. S-002-5), we recommend the use of PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2) for optimal performance.  \n2. Warm the supplemented culture medium to 37°C in a water bath.  \n3. Remove the vial of cells to be thawed from liquid nitrogen and rapidly thaw by placing at 37°C in a water bath with gentle agitation for 1–2 minutes (or once a sliver of ice is left i","cbCaio7tHp90jtkQ","https://ap.wps.com/l/cbCaio7tHp90jtkQ","pdf",104584,"English","# Product description\n## Intended use\n## Storage and stability\n## Caution\n# Initiate cultures from cryopreserved cells\n## Sample protocol steps\n# Maintain stock cultures\n## Medium change schedule and confluence targets","[{\"question\":\"What does each vial of HEMa-LP contain and how are the cells prepared?\",\"answer\":\"Each vial contains at least 5 × 105 viable HEMa-LP cells cryopreserved after the secondary culture stage in a DMSO-containing medium. Cells are thawed for use in culture work.\"},{\"question\":\"How are contamination and specific agents checked before the product is released?\",\"answer\":\"An independent laboratory tests for mycoplasma, Hepatitis B, Hepatitis C, and HIV-1, and those agents were not detected. The product is also performance tested by culturing through multiple passages and verifying no bacterial, yeast, or fungal contamination during the culture period.\"},{\"question\":\"What are the key recommendations for storage and handling after shipping?\",\"answer\":\"HEMa-LP should arrive frozen on dry ice and be placed immediately into liquid nitrogen vapor phase storage. Handling guidance includes wearing protective eyewear, gloves, and a lab coat, and following the document’s caution statements for treating all human cells as potential pathogens.\"}]","Human Epidermal Melanocytes, adult (HEMa-LP) - Product Catalog and Protocol | PDF"]