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Using public TCGA and GEO datasets, then validating key CpGs by pyrosequencing in two OSCC cohorts, higher 3′UTR methylation correlated with smoking habits and overall survival, supporting HOXA1 gene body methylation as a prognostic biomarker for clinical monitoring.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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Oral Squamous cell Carcinoma  \nBruna Pereira Sorroche 1,†, Keila Cristina Miranda 2,†, Caroline Moraes Beltrami 3,  \nLidia Maria Rebolho Batista Arantes 1, Luiz Paulo Kowalski 4,5, Fabio Albuquerque Marchi 6,7, Silvia Regina Rogatto 8,9,‡ and Janete Dias Almeida 2, *,‡  \nCitation: Sorroche, B.P.; Miranda, K.C.; Beltrami, C.M.; Arantes,  \nL.M.R.B.; Kowalski, L.P.; Marchi, F.A.; Rogatto, S.R.; Almeida, J.D. HOXA1 3′UTR Methylation Is a Potential Prognostic Biomarker in Oral Squamous cell Carcinoma. Cancers 2024, 16, 874. [https://doi.org/](https://doi.org/)[ ](https://doi.org/)[10.3390/cancers16050874](10.3390/cancers16050874)  \nAcademic Editor: Markus Heppt  \nReceived: 11 January 2024  \nRevised: 15 February 2024  \nAccepted: 19 February 2024  \nPublished: 22 February 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil; [bruna.sorroche20@edu.hospitaldeamor.com.br](bruna.sorroche20@edu.hospitaldeamor.com.br) (B.P.S.); [lidia.arantes@hospitaldeamor.com.br](lidia.arantes@hospitaldeamor.com.br) (L.M.R.B.A.)  \n2 Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12224-300, Brazil; [keilamiranda94@gmail.com](keilamiranda94@gmail.com)  \n[3](3 International Research Center)[ International Research Center](3 International Research Center), [A.C](A.C). Camargo Cancer Center, São Paulo 01508-010, Brazil; [cahbeltrami@gmail.com](cahbeltrami@gmail.com)  \n4 Head and Neck Surgery and Otorhinolaryngology Department, AC Camargo Cancer Center, Latin American Cooperative Oncology Group, São Paulo 01509-010, Brazil; [lpkowalski@accamargo.org.br](lpkowalski@accamargo.org.br)  \n5 Head and Neck Surgery Department and LIM 28, University of São Paulo Medical School, São Paulo 01246-903, Brazil  \n6 Center for Translational Research in Oncology, Cancer Institute of the State of São Paulo (ICESP), São Paulo 01246-000, Brazil; [fabio.marchi@hc.fm.usp.br](fabio.marchi@hc.fm.usp.br)  \n7 Clinical Hospital of the University of Sao Paulo Medical School (HCFMUSP), São Paulo 05403-010, Brazil  \n8 Department of Clinical Genetics, University Hospital of Southern Denmark, 7100 Vejle, Denmark; [silvia.regina.rogatto@rsyd.dk](silvia.regina.rogatto@rsyd.dk)  \n9 Institute of Regional Health Research, University of Southern Denmark, 5230 Odense, Denmark  \n* [Correspondence: janete.almeida@unesp.br](Correspondence: janete.almeida@unesp.br)[ ](Correspondence: janete.almeida@unesp.br)† These authors contributed equally to this work.  \n‡ These authors also contributed equally to this work.  \nSimple Summary: Head and neck cancer with increased expression levels of HOXA1 tends to have a worse outcome. This gene is regulated by DNA methylation. Herein, we studied the methylation pattern of HOXA1 in oral cavity tumors. We found that methyl groups gather more often in a specific region of HOXA1, named 3′UTR, in tumor cells compared to healthy tissues. Interestingly, patients with increased methylation levels were preferentially detected in heavier smokers and patients with a longer survival. Our findings suggest that the analysis of the DNA methylation located in the 3′UTR of HOXA1 could help predict the patient’s prognosis. Our findings could potentially guide treatment decisions and improve patient care in the future.  \nAbstract: Background: HOXA1 is a prognostic marker and a potential predictive biomarker for radioresistance in head and neck tumors. Its overexpression has been associated with promoter methylation","cbCaimSvL60b7HXz","https://ap.wps.com/l/cbCaimSvL60b7HXz","pdf",3000948,14,"English","# Introduction\n# Simple Summary\n# Abstract\n# Materials and Methods\n# Results\n# Conclusions","[{\"question\":\"What did the study focus on regarding HOXA1 methylation in OSCC?\",\"answer\":\"The study analyzed HOXA1 DNA methylation across different genomic regions in oral squamous cell carcinoma and examined CpG-site methylation patterns in relation to clinical data.\"},{\"question\":\"How was HOXA1 DNA methylation measured and validated?\",\"answer\":\"Methylation status was evaluated using TCGA and three GEO datasets, then validated by pyrosequencing in two independent OSCC patient cohorts of 15 and 47 patients.\"},{\"question\":\"What clinical associations were found with higher HOXA1 3′UTR methylation?\",\"answer\":\"Higher HOXA1 3′UTR methylation in tumor samples correlated positively with smoking habits and patients’ overall survival.\"}]","HOXA1 3′UTR Methylation Is a Potential Prognostic Biomarker in Oral Squamous cell Carcinoma | PDF",1789995748,35]