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METHODS: HNRNPA2B1 expression was assessed by RT-qPCR and TCGA, while functional effects were tested using CCK-8, colony formation, and scratch assays, complemented by in vivo experiments and survival analyses.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/hnrnpa2b1-induces-cell-proliferation-and-acts-as-biomarker-in-breast-cancer/333796/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/hnrnpa2b1-induces-cell-proliferation-and-acts-as-biomarker-in-breast-cancer/333796.png","ImageObject",300,407,{"name":42,"@type":43},"Asher","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-06","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What was the study’s main objective regarding HNRNPA2B1 in breast cancer?","Question",{"text":63,"@type":64},"To identify the role of HNRNPA2B1 in breast cancer, including its effects on tumor cell behavior and its value as a biomarker.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How did researchers measure HNRNPA2B1 expression and validate its function?",{"text":68,"@type":64},"HNRNPA2B1 expression was investigated using RT-qPCR and TCGA data, and its functional effects were evaluated with CCK-8, colony formation, and scratch assays, along with in vivo tumor growth assessment.",{"name":70,"@type":61,"acceptedAnswer":71},"What did the results show about HNRNPA2B1 in breast cancer patients?",{"text":72,"@type":64},"HNRNPA2B1 was highly expressed and induced cancer cell proliferation and migration, and its expression was associated with patient characteristics and prognosis.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},333796,1790241160,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":55,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":112,"preview_page_count":4},687197207639,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","Cancer Biomarkers 40 (2024) 285–296 285  \nDOI 10.3233/CBM-230576 IOS Press  \nHNRNPA2B1 induces cell proliferation and acts as biomarker in breast cancer  \nYi Yanga ;b ; 1 , Yi Zhangc ; 1 , Tongbao Fenga ;d ;􀀃 and Chunfu Zhuc ;d ;􀀃  \na Department of Clinical Laboratory, The Affiliated Changzhou Second People's Hospital, Nanjing Medical University, Changzhou, China  \nb Department of Blood Transfusion Medicine, School of Medicine, Jinling Hospital, Nanjing University, Nanjing, Jiangsu, China  \nc Department of General Surgery, The Affiliated Changzhou Second People's Hospital, Nanjing Medical University, Changzhou, China  \ndThe Affiliated Changzhou Second People's Hospital, Nanjing Medical University, Changzhou Medical Center, Nanjing Medical University, Jiangsu, China  \nReceived 30 January 2024 Accepted 19 July 2024  \nAbstract.  \nBACKGROUND: Numerous studies have shown that m6A plays an important regulatory role in the development of tumors. HNRNPA2B1, one of the m6A RNA methylation reading proteins, has been proven to be elevated in human cancers.  \nOBJECTIVE: In this study, we aimed to identify the role of HNRNPA2B1 in breast cancer.  \nMETHODS: HNRNPA2B1 expression was investigated via RT-qPCR and TCGA database in breast cancer. Then, the function of HNRNPA2B1 on cancer cell was measured by CCK8 assays, colony formation and scratch assays. In addition, HNRNPA2B1 expression in BRCA was explored via the Wilcoxon signed-rank test, KruskalWallis test and logistic regression. The association with HNRNPA2B1 expression and survival were considered by KaplanMeier and Cox regression analyses. The biological function of HNRNPA2B1 was analyzed via gene set enrichment analysis (GSEA) and the cluster Profiler R software package.  \nRESULTS: We found that HNRNPA2B1 was highly expressed and induced cell proliferation and migration in breast cancer. Moreover, we observed HNRNPA2B1 induced tumor growth in vivo. In addition, we also found HNRNPA2B1 expression was associated with characteristics and prognosis in breast cancer patients.  \nCONCLUSION: Our findings suggested that HNRNPA2B1 promoted tumor growth and could function as a new potential molecular marker in breast cancer.  \nKeywords: HNRNPA2B1, cell proliferation, molecular marker, breast cancer  \n1Yi Yang, Yi Zhang contributed equally to this work.  \n􀀃 Corresponding authors: Tongbao Feng, Department of Clinical Laboratory, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, 168 Gehu Avenue, Changzhou, China. Tel.: +86 519 81087371; E-mail: [fengtongbao@njmu.edu.cn. Chunfu](fengtongbao@njmu.edu.cn. Chunfu)[ ](fengtongbao@njmu.edu.cn. Chunfu)Zhu, Department of General Surgery, the Affiliated Changzhou Second People's Hospital of Nanjing Medical University, 168 Gehu Avenue, Changzhou, China. Tel.: +86 519 81087259; Fax: +86 519 81087710; E-mail: [zcfmlm@njmu.edu.cn](zcfmlm@njmu.edu.cn).  \n1. Introduction  \nBreast cancer is the most common malignant tumor in women [1] . The majority of breast cancer is invasive carcinoma (BRCA), which accounts for approximately 80%[2] . During the development of cancer, the cancer shows the heterogeneity of metastasis, with obvious metastasis advantages in different organs, leading to differences in the prognosis and treatment response of cancer patients [3] . Approximately 30% of breast cancers eventually develop into advanced cancer, and 90%  \nISSN 1574-0153 􀀍c 2024 – The authors. Published by IOS Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC 4 .0) .  \n286 Y. Yang et al. / The Role of HNRNPA2B1 in breast cancer  \nof cancer deaths are attributed to primary metastases and the 5-year overall survival rate of patients without distant metastasis is more than 80%[4] .  \nSignificant heterogeneity differences in breast cancer have been recognized via advances in cancer diagnosis and treatment (Yeo and Guan, 2017) . Recent study showed that precision medicine","cbCaihWREKz9bfCv","https://ap.wps.com/l/cbCaihWREKz9bfCv","pdf",65559743,12,"English","# Introduction\n## Breast cancer background\n## m6A and HNRNPA2B1 rationale\n# Materials and methods\n## Cell culture","[{\"question\":\"What was the study’s main objective regarding HNRNPA2B1 in breast cancer?\",\"answer\":\"To identify the role of HNRNPA2B1 in breast cancer, including its effects on tumor cell behavior and its value as a biomarker.\"},{\"question\":\"How did researchers measure HNRNPA2B1 expression and validate its function?\",\"answer\":\"HNRNPA2B1 expression was investigated using RT-qPCR and TCGA data, and its functional effects were evaluated with CCK-8, colony formation, and scratch assays, along with in vivo tumor growth assessment.\"},{\"question\":\"What did the results show about HNRNPA2B1 in breast cancer patients?\",\"answer\":\"HNRNPA2B1 was highly expressed and induced cancer cell proliferation and migration, and its expression was associated with patient characteristics and prognosis.\"}]","HNRNPA2B1 induces cell proliferation and acts as biomarker in breast cancer | PDF",1790004145]