[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-640797-105":3,"detail-sidebar-cat-0-en-105":80,"doc-detail-640797-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":73,"head_meta":75,"extra_data":77,"updated_unix":79},105,"en","histrap-hp-instructions-71-5027-68-af","HisTrap HP - Instructions 71-5027-68 AF","","HisTrap HP ready-to-use Ni Sepharose High Performance affinity columns support preparative purification of histidine-tagged recombinant proteins using immobilized metal ion affinity chromatography (IMAC). The Ni Sepharose matrix is designed for fast, simple separations and low Ni2+ leakage, with compatibility across common protein purification additives and buffers. Columns can be operated with syringe, peristaltic pump, or ÄKTA/FPLC systems, and include guidance on connector kits, flow requirements, chemical stability, pH stability, storage, stripping and recharging, cleaning-in-place, scaling-up, troubleshooting, and ordering information.",{"@graph":14,"@context":72},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/histrap-hp-instructions-71-5027-68-af/640797/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/histrap-hp-instructions-71-5027-68-af/640797.png","ImageObject",300,407,{"name":42,"@type":43},"lilac","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-06","2026-10-22",true,{"@type":52,"interactionType":53,"userInteractionCount":33},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58,64,68],{"name":59,"@type":60,"acceptedAnswer":61},"What is HisTrap HP used for?","Question",{"text":62,"@type":63},"HisTrap HP is used for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).","Answer",{"name":65,"@type":60,"acceptedAnswer":66},"How can the HisTrap HP columns be operated?",{"text":67,"@type":63},"They can be operated with a syringe (using the supplied luer adapter), a peristaltic pump, or a chromatography system such as ÄKTAdesign or FPLC systems. A tight adapter helps prevent leakage.",{"name":69,"@type":60,"acceptedAnswer":70},"What safety caution applies to HisTrap HP?",{"text":71,"@type":63},"The columns contain nickel and may produce an allergic reaction.","https://schema.org",{"og:url":32,"og:type":74,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":76,"canonical":32},"index,follow",{"doc_id":78,"site_id":7},640797,1792711601,{"code":4,"msg":81,"data":82},"success",[83,87,91,95,100,105,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":88,"show_sort_weight":89,"slug":90},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":92,"show_sort_weight":93,"slug":94},"Exam",70,"exam",{"id":96,"doc_module":4,"doc_module_name":25,"category_name":97,"show_sort_weight":98,"slug":99},5,"Comic",60,"comic",{"id":101,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},6,"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":107,"slug":108},7,40,"healthcare",{"id":110,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},8,"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":96,"slug":129},19,"General","general",{"code":4,"msg":81,"data":131},{"doc_id":78,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":106,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":33,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":117,"language":139,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":12,"update_tm":79,"read_time":103,"preview_page_count":4},3985747861865,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","GE Healthcare  \nInstructions 71-5027-68 AF HisTrap affinity columns  \nHisTrap HP, 1 ml and 5 ml  \nHisTrap™ HP is a ready to use column, prepacked with precharged Ni Sepharose™ High Performance. This prepacked column is ideal for preparative purification of Histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC) .  \nThe special design of the column, together with the high-performance matrix of the Ni Sepharose medium, provides fast, simple, and easy separations in a convenient format.  \nNi Sepharose High Performance has low nickel (Ni2+) ion leakage and is compatible with a wide range of additives used in protein purification.  \nHP columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTAdesign™ chromatography systems or FPLC™ System.  \nCaution!  \nContains nickel. May produce an allergic reaction.  \nCode No.  \nProduct  \nNo. supplied  \n\n| 17-5247-01 | HisTrap HP 1 ml | 5 × 1 ml |\n| --- | --- | --- |\n| 17-5247-05 | HisTrap HP 1 ml | 100 × 1 ml* |\n| 17-5248-01 | HisTrap HP 5 ml | 1 × 5 ml |\n| 17-5248-02 | HisTrap HP 5 ml | 5 × 5 ml |\n| 17-5248-05 | HisTrap HP 5 ml | 100 × 5 ml* |\n| * Pack size available by special order. |  |  |\n| Connector kit\u003Cbr>Connectors supplied | Usage | No. supplied |\n| 1/16” male/luer female | Connection of syringe to top of HiTrap column | 1 |\n| Tubing connector flangeless/M6 female | Connection of tubing (e. g. Peristaltic Pump P1) to bottom of HiTrap column* | 1 |\n| Tubing connector flangeless/M6 male | Connection of tubing (e. g. Peristaltic Pump P1) to top of HiTrap column** | 1 |\n| Union 1/16” female/ | Connection to original FPLC System |  |\n| M6 male | through bottom of HiTrap column | 1 |\n| Union M6 female/ | Connection to original FPLC System |  |\n| 1/16” male | through top of HiTrap column | 1 |\n| Stop plug female, 1/16” | Sealing bottom of HiTrap column | 2, 5 or 7 |\n\n* Union 1/16” female/M6 male is also needed.  \n** Union M6 female/1/16” male is also needed. Table of contents  \n1. Description 3  \n2. General Considerations 6  \n3. Operation 7  \n4. Optimization 11  \n5. Stripping and recharging 12  \n6. Cleaning-in-place 13  \n7. Scaling-up 13  \n8. Storage 14  \n9. Troubleshooting 14  \n10. Further Information 17  \n11. Ordering Information 18  \n1. Description  \nMedium properties  \nHisTrap HP 1 ml and 5 ml columns are prepacked with Ni Sepharose High Performance, which consists of 34 µm highly cross-linked agarose beads with an immobilized chelating group. The medium has then been charged with Ni2+ -ions.  \nSeveral amino acids, for example histidine, form complexes with many metal ions. Ni Sepharose High Performance selectively binds proteins if suitable complex-forming amino acid residues are exposed on the protein surface. Additional histidines, such as in the case of (histidine)6-tag, increase affinity for Ni2+ and generally make the histidine-tagged protein the strongest binder among other proteins in e. g. an E. coli extract.  \nColumn properties  \nHisTrap HP columns are made of biocompatible polypropylene that does not interact with biomolecules. Columns are delivered with a stopper on the inlet and a snap-off end on the outlet. The columns have porous top and bottom frits that allow high flow rates. They cannot be opened or refilled. Columns can be operated with either a syringe and the supplied luer adapter, a peristaltic pump, or a chromatography system such as ÄKTAdesign or FPLC System.  \nNote: To prevent leakage, ensure that the adapter is tight.  \nTable 1. HisTrap HP characteristics.  \n\n| Matrix\u003Cbr>Average particle size Metal ion capacity Dynamic binding\u003Cbr>capacity* Column volumes\u003Cbr>Column dimensions\u003Cbr>Recommended flow rate Max. flow rates Max back pressure†† Compatibility during use\u003Cbr>Chemical stability§§§\u003Cbr>Avoid in buffers pH stability§§§\u003Cbr>Storage\u003Cbr>Storage temperature | Highly cross-linked spherical agarose, 6%\u003Cbr>34 µm\u003Cbr>~15 µmol Ni2+/ml medium\u003Cbr>At least 40 mg (histidine)6-tagged protein/ml med","cbCaihjHdDofG2dy","https://ap.wps.com/l/cbCaihjHdDofG2dy","pdf",187193,"English","# 1. Description\n## Medium properties\n## Column properties\n# 2. General Considerations\n# 3. Operation\n# 4. Optimization\n# 5. Stripping and recharging\n# 6. Cleaning-in-place\n# 7. Scaling-up\n# 8. Storage\n# 9. Troubleshooting\n# 10. Further Information\n# 11. Ordering Information","[{\"question\":\"What is HisTrap HP used for?\",\"answer\":\"HisTrap HP is used for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).\"},{\"question\":\"How can the HisTrap HP columns be operated?\",\"answer\":\"They can be operated with a syringe (using the supplied luer adapter), a peristaltic pump, or a chromatography system such as ÄKTAdesign or FPLC systems. A tight adapter helps prevent leakage.\"},{\"question\":\"What safety caution applies to HisTrap HP?\",\"answer\":\"The columns contain nickel and may produce an allergic reaction.\"}]","HisTrap HP - Instructions 71-5027-68 AF | PDF"]