[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-145833-en":3,"doc-seo-145833-105":30,"detail-sidebar-cat-0-en-105":92},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":20,"is_downloadable":20,"audit_status":20,"page_count":21,"language":22,"language_code":23,"site_id":24,"html_lang":23,"table_of_contents":25,"faqs":26,"seo_title":27,"seo_description":14,"update_tm":28,"read_time":29},145833,687207022233,"Connor ","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d",7,"Healthcare","Histostain SAP Kit","Histostain SAP kits (In Vitro Diagnostic) provide a labeled-[strept]Avidin-Biotin (LAB-SA) staining system to visualize antigens bound by a user-supplied primary antibody on human tissue or cell samples. The method uses a biotinylated secondary antibody and an alkaline phosphatase–conjugated streptavidin, producing an intense chromogen color deposit. Includes intended-use guidance, background on streptavidin–biotin binding advantages, storage conditions, supplied reagents, and recommended staining preparation steps for reliable interpretation.","Histostain® -SAP Kit  \nInvitrogen® LAB-SA Detection System  \n| CAT. NO. | FOR PRIMARY ANTIBODY | CHROMOGEN | GOOD FOR |\n| --- | --- | --- | --- |\n| 95-6142 | Rabbit | AP-Red | 150 slides |\n\nINTENDED USE  \nFor In Vitro Diagnostic Use. Interpretation must be made within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.  \nHistostain® kits are designed to reveal antigens that have reacted with a user-supplied primary antibody on human tissue or cell samples. Frozen or paraffin-embedded tissues, freshly prepared lymphocytes, and fixed cultured cells can be used.  \nBACKGROUND  \nInvitrogen’s HISTOSTAIN® SAP kits stain tissues using the Labeled-[strept]Avidin-Biotin (LAB-SA) method,1 also known as StreptavidinBiotin Amplification. This design employs Alkaline Phosphatase (AP), streptavidin, and a two-tiered antibody technique to reveal the presence of antigens in a variety of human tissues and cell preparations. After a user-supplied primary antibody has been bound to a target antigen, the LABSA method uses an affinity-purified and biotinylated secondary antibody which binds specifically to that primary antibody. AP labeled streptavidinis then bound to this biotinylated secondary antibody, and the entire complex is revealed by adding a substrate/chromogen mixture which createsan intense color deposit through the activity of the bound enzyme. LAB-SA methodology is now widely used in diagnostic medicine and basic research. Streptavidin, the protein used in this technique, is isolated from Streptomyces avidinii,2 and is similar to egg white avidin in its binding properties. A molecule of streptavidin (60 kDa) has 4 subunits, each of which can bind 1 biotin molecule. Biotin is a water-soluble vitamin (244 Da) which can be coupled to a variety of proteins and nucleic acids (e.g., immunoglobulins, hormone receptors, cDNA and DNA probes, etc.) .  \nThis system is based on the unusually strong binding (Kd=10-15 M) between streptavidin and biotin. The advantage to using streptavidin instead of egg white avidin is the elimination of avidin’s inherent non-specific binding properties in certain tissue samples.3 Streptavidin has an isoelectric point of approximately 6.5 while avidin’s is nearly 10. Therefore, streptavidin will have a neutral charge under physiological conditions, and exhibit lower non-specific binding than avidin. In avidin, non-specific binding is also contributed by the interaction of carbohydrate side-chains. The lack of carbohydrate in streptavidin results in lower background and a higher signal-to-noise ratio. In addition to lower background, LAB-SA provides superior sensitivity to that of the Avidin-Biotin Complex (ABC) . 1,4-6 The LAB-SA method is also more sensitive than the peroxidase (PAP) method,1,6 and has the advantage of a single universal labeling reagent (enzyme conjugated streptavidin), instead of specific PAP7 complexes for each animal system.  \nSTORAGE AND SHELF LIFE  \nStore kit at 2-8°C. All performance claims are void after kit expiration date.  \nREAGENTS SUPPLIED  \nReagent 1A. One dropper bottle (15 mL) of ready-to-use serum blocking solution, 10% goat non-immune serum Reagent 1B. One dropper bottle (15 mL) of ready-to-use biotinylated second antibody  \nReagent 2. One dropper bottle (15 mL) of ready-to-use streptavidin-alkaline phosphatase conjugate  \nReagent 3A. One dropper bottle (2 mL) of AP-Red enhancer (40x)  \nReagent 3B. One dropper bottle (2 mL) of AP-Red concentrate (40x)  \nReagent 3C. One dropper bottle (3 mL) of substrate solution concentrate (20x) Reagent 4. One dropper bottle (15 mL) of ready-to-use Hematoxylin solution Reagent 5. One bottle (15 mL) of ready-to-use GVA Mount  \nREAGENTS AND MATERIALS REQUIRED BUT NOT PROVIDED  \n– Xylene, ethanol, and absolute methanol  \n– Distilled or deionized water  \n– 10 mM phosphate-buffered saline (PBS), pH 7.4 or 50 mM Tris-buffer saline (TBS), pH 7.8. Optional: 0.05% Tween 20.  \n– Mini PAP Pen (Invitrogen Cat","cbCaig0bQtBUYYf5","https://ap.wps.com/l/cbCaig0bQtBUYYf5","pdf",167053,1,11,"English","en",105,"# Intended use\n# Background\n## Streptavidin–biotin binding rationale\n# Storage and shelf life\n# Reagents supplied\n# Reagents and materials required but not provided\n# Suggested staining protocol\n## Preliminary preparation of slides\n## Slide preparation\n## Deparaffinization and rehydration","[{\"question\":\"What is the intended use of the Histostain SAP kit?\",\"answer\":\"For in vitro diagnostic use. Antigen staining is performed on human tissue or cell samples, and interpretation must be made within the context of clinical history and other diagnostic tests by a qualified pathologist.\"},{\"question\":\"How does the LAB-SA method work in this kit?\",\"answer\":\"A primary antibody binds the target antigen, followed by a biotinylated secondary antibody. Alkaline phosphatase–labeled streptavidin binds the biotin, and a substrate/chromogen mixture produces a colored deposit.\"},{\"question\":\"Why is streptavidin preferred over egg white avidin?\",\"answer\":\"Streptavidin eliminates avidin’s inherent non-specific binding in certain samples. Its lower background and higher signal-to-noise ratio improve staining sensitivity compared with related avidin-biotin approaches.\"}]","Histostain SAP Kit | PDF",1787730282,28,{"code":4,"msg":31,"data":32},"ok",{"site_id":24,"language":23,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":87,"head_meta":89,"extra_data":91,"updated_unix":28},"histostain-sap-kit","",{"@graph":36,"@context":86},[37,54,69],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,48,51],{"item":41,"name":42,"@type":43,"position":20},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":47},"https://docshare.wps.com/document/","Document",2,{"item":49,"name":12,"@type":43,"position":50},"https://docshare.wps.com/document/healthcare/",3,{"item":52,"name":13,"@type":43,"position":53},"https://docshare.wps.com/document/histostain-sap-kit/145833/",4,{"url":52,"name":13,"@type":55,"author":56,"headline":13,"publisher":58,"fileFormat":61,"inLanguage":23,"description":14,"dateModified":62,"datePublished":63,"encodingFormat":61,"isAccessibleForFree":64,"interactionStatistic":65},"DigitalDocument",{"name":9,"@type":57},"Person",{"url":41,"name":59,"@type":60},"DocShare","Organization","application/pdf","2026-08-30","2026-08-26",true,{"@type":66,"interactionType":67,"userInteractionCount":20},"InteractionCounter",{"@type":68},"ViewAction",{"@type":70,"mainEntity":71},"FAQPage",[72,78,82],{"name":73,"@type":74,"acceptedAnswer":75},"What is the intended use of the Histostain SAP kit?","Question",{"text":76,"@type":77},"For in vitro diagnostic use. Antigen staining is performed on human tissue or cell samples, and interpretation must be made within the context of clinical history and other diagnostic tests by a qualified pathologist.","Answer",{"name":79,"@type":74,"acceptedAnswer":80},"How does the LAB-SA method work in this kit?",{"text":81,"@type":77},"A primary antibody binds the target antigen, followed by a biotinylated secondary antibody. Alkaline phosphatase–labeled streptavidin binds the biotin, and a substrate/chromogen mixture produces a colored deposit.",{"name":83,"@type":74,"acceptedAnswer":84},"Why is streptavidin preferred over egg white avidin?",{"text":85,"@type":77},"Streptavidin eliminates avidin’s inherent non-specific binding in certain samples. Its lower background and higher signal-to-noise ratio improve staining sensitivity compared with related avidin-biotin approaches.","https://schema.org",{"og:url":52,"og:type":88,"og:title":13,"og:site_name":59,"og:description":14},"article",{"robots":90,"canonical":52},"index,follow",{"doc_id":7,"site_id":24},{"code":4,"msg":5,"data":93},[94,98,102,106,111,116,119,124,129,132,136],{"id":20,"doc_module":4,"doc_module_name":46,"category_name":95,"show_sort_weight":96,"slug":97},"Story & Novel",90,"story-novel",{"id":47,"doc_module":4,"doc_module_name":46,"category_name":99,"show_sort_weight":100,"slug":101},"Literature",80,"literature",{"id":53,"doc_module":4,"doc_module_name":46,"category_name":103,"show_sort_weight":104,"slug":105},"Exam",70,"exam",{"id":107,"doc_module":4,"doc_module_name":46,"category_name":108,"show_sort_weight":109,"slug":110},5,"Comic",60,"comic",{"id":112,"doc_module":4,"doc_module_name":46,"category_name":113,"show_sort_weight":114,"slug":115},6,"Technology",50,"technology",{"id":11,"doc_module":4,"doc_module_name":46,"category_name":12,"show_sort_weight":117,"slug":118},40,"healthcare",{"id":120,"doc_module":4,"doc_module_name":46,"category_name":121,"show_sort_weight":122,"slug":123},8,"Research & Report",30,"research-report",{"id":125,"doc_module":4,"doc_module_name":46,"category_name":126,"show_sort_weight":127,"slug":128},9,"Religion & Spirituality",20,"religion-spirituality",{"id":127,"doc_module":4,"doc_module_name":46,"category_name":130,"show_sort_weight":127,"slug":131},"World Cup","world-cup",{"id":133,"doc_module":4,"doc_module_name":46,"category_name":134,"show_sort_weight":133,"slug":135},10,"Lifestyle","lifestyle",{"id":137,"doc_module":4,"doc_module_name":46,"category_name":138,"show_sort_weight":107,"slug":139},19,"General","general"]