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The study presents a high-throughput tissue lipid sampling method using electroporation-based biopsy (e-biopsy) to enable lipid collection from human skin samples. Methods: Lipids were identified from 12 patients across 13 samples, then analyzed by UPLC-MS-MS to generate lipidomic profiles for cSCC, BCC, and healthy skin. Results: 27 lipids differed between groups (p \u003C 0.05).",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & 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problem does the study address in diagnosing skin cancers?","Question",{"text":63,"@type":64},"It targets the rising incidence of cSCC and BCC while noting that the current diagnostic workflow is time-consuming.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does e-biopsy enable lipidomics in this work?",{"text":68,"@type":64},"It uses electroporation to permeabilize cells for rapid sampling of tissue lipids, which are then profiled using UPLC-MS-MS.",{"name":70,"@type":61,"acceptedAnswer":71},"Which lipidomic trends distinguish cancers from healthy skin in the results?",{"text":72,"@type":64},"Comparative analysis shows low diglycerides in both cSCC and BCC, higher phospholipids in BCC, and higher lyso-phospholipids in cSCC versus healthy 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electroporation-based biopsy differentiate healthy skin, cutaneoussquamous cell carcinoma, and basal cell carcinoma  \nLeetal Louie1  Zohar Yakhini4, 5  \nJulia Wise1  Ariel Berl2   Ofir Shir-az2  Avshalom Shalom2  Alexander Golberg1  \nVladimir Kravtsov3  Edward Vitkin1,4  \n1 Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel  \n2 Department of Plastic Surgery, Meir Medical Center, Kfar Sava, Israel  \n3 Department of Pathology, Meir Medical Center, Kfar Sava, Israel  \n4Arazi School of Computer Science, Reichman University, Herzliya, Israel  \n5 Department of Computer Science, TechnionIsrael Institute of Technology, Haifa, Israel  \nCorrespondence  \nAlexander Golberg and Edward Vitkin, Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. Email: [agolberg@tauex.tau.ac.il](agolberg@tauex.tau.ac.il) and [edward.vitkin@gmail.com](edward.vitkin@gmail.com)  \nFunding information  \nIsrael Innovation Authority Kamin project; TAU SPARK fund; TAU Zimin Center for technologies for better life,; EuroNanoMed MATISSE  \nAbstract  \nBackground: The incidence rates of cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC) skin cancers are rising, while the current diagnostic process is time-consuming. We describe the development of a novel approach to high-throughput sampling of tissue lipids using electroporation-based biopsy, termed e-biopsy. We report on the ability of the e-biopsy technique to harvest large amounts of lipids from human skin samples.  \nMaterials and Methods: Here, 168 lipids were reliably identified from 12 patients providing a total of 13 samples. The extracted lipids were profiled with ultra-performance liquid chromatography and tandem mass spectrometry (UPLC-MS-MS) providing cSCC, BCC, and healthy skin lipidomic profiles.  \nResults: Comparative analysis identified 27 differentially expressed lipids (p \u003C 0.05). The general profile trend is low diglycerides in both cSCC and BCC, high phospholipids in BCC, and high lyso-phospholipids in cSCC compared to healthy skin tissue samples. Conclusion: The results contribute to the growing body of knowledge that can potentially lead to novel insights into these skin cancersand demonstrate the potential of thee-biopsy technique for the analysis of lipidomic profiles of human skin tissues.  \nKEYWORDS  \nbasal cell carcinoma, cutaneoussquamous cell carcinoma, e-biopsy, electroporation-based biopsy, high-throughput lipidomics, lipidomic profiles  \n1  INTRODUCTION  \nRising trends in keratinocyte carcinoma (cSCC and BCC) incidence have been observed.1–5 These are among the most common cancers  \nAbbreviations: BCC, basal cell carcinoma; Cer, ceramides; CerG2, diglycosylceramide; cSCC, cutaneous squamous cell carcinoma; DG, diglyceride; e-biopsy, electroporation-based biopsy; LPC, lyso-phosphatidylcholine; LPE, lyso-phosphatidylethanolamine; LPI,  \nlyso-phosphatidylinositol; PC, phosphatidylcholine; PI, phosphatidylinositol; PS, phosphatidylserine; TG, triglyceride; UPLC-MS-MS, ultra performance liquid chromatography and tandem mass spectrometry.  \ndiagnosed, but due to their low mortality rate, they are usually excluded from cancer registries,4 despite their impact on quality of life and risks of premature mortality.2,6 These trends foreshadow increasing wait time for diagnosis under the current gold standard diagnostic method which relies on excision and histopathological examination, with advanced technologies acting as support.7 The current gold standard is problematic when subsequent treatment requires electrodesiccation and cautery,8 so finding less invasive methods for differentiating between healthy and cancerous skin, as well as between cancer types  \nThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, p","cbCailyq57mDgbOM","https://ap.wps.com/l/cbCailyq57mDgbOM","pdf",1198637,"English","# Abstract\n## Background\n## Materials and Methods\n## Results and Conclusion\n# Keywords\n# 1 INTRODUCTION\n## Incidence and diagnostic challenges\n## Rationale for lipidomic profiling and e-biopsy","[{\"question\":\"What problem does the study address in diagnosing skin cancers?\",\"answer\":\"It targets the rising incidence of cSCC and BCC while noting that the current diagnostic workflow is time-consuming.\"},{\"question\":\"How does e-biopsy enable lipidomics in this work?\",\"answer\":\"It uses electroporation to permeabilize cells for rapid sampling of tissue lipids, which are then profiled using UPLC-MS-MS.\"},{\"question\":\"Which lipidomic trends distinguish cancers from healthy skin in the results?\",\"answer\":\"Comparative analysis shows low diglycerides in both cSCC and BCC, higher phospholipids in BCC, and higher lyso-phospholipids in cSCC versus healthy samples.\"}]","High-throughput lipidomic profiles sampled with electroporation-based biopsy differentiate healthy skin, cutaneous squamous cell carcinoma, and basal cell carcinoma | PDF",1789996482,23]