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High BBOX1 in paracancerous tissue correlates with poorer overall and disease-free survival, suggesting prognostic value for HCC outcomes.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/high-expression-of-bbox1-in-paracancerous-tissue-is-associated-with-poor-prognosis-in-hepatocellular-carcinoma-patients/331534/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/high-expression-of-bbox1-in-paracancerous-tissue-is-associated-with-poor-prognosis-in-hepatocellular-carcinoma-patients/331534.png","ImageObject",300,407,{"name":42,"@type":43},"Quinn","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-27","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",7,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is the main focus of the study regarding BBOX1?","Question",{"text":63,"@type":64},"The study evaluates whether BBOX1 expression in tumor and paracancerous tissues has clinical prognostic significance for hepatocellular carcinoma patients.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How were BBOX1 mRNA and protein levels assessed?",{"text":68,"@type":64},"BBOX1 mRNA expression was analyzed using bioinformatics, and BBOX1 protein levels in paired tumor and paracancerous tissues were determined by immunohistochemistry in 83 HCC patients.",{"name":70,"@type":61,"acceptedAnswer":71},"What relationship does BBOX1 expression show with patient survival outcomes?",{"text":72,"@type":64},"Low BBOX1 mRNA expression in HCC is associated with poorer overall survival, and high BBOX1 expression in paracancerous tissues correlates with worse overall survival and disease-free 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tissue is associated with poor prognosis in hepatocellular carcinoma patients  \nRunqi Fang1, Yuling Zhan2,3, Xiang Dong2,3, Suwan Li2,3, Minghui Yang2,4, Yunxia Zhao5 & Yu Gao2,6,7􀀍  \nThe molecular profile of paracancerous tissue has been reported to be prognostic for recurrence in patients with different cancers. This study investigated the clinical significance of the protein expression of gamma-butyrobetaine hydroxylase 1 (BBOX1) in tumor and paracancerous tissues of hepatocellular carcinoma (HCC) patients. This study assessed the role of BBOX1 mRNA in tumor and paracancerous tissues in HCC via bioinformatics analysis. The protein levels of BBOX1 in paired tumor and paracancerous tissues from 83 HCC patients were determined by immunohistochemistry. The associations among BBOX1 protein levels, clinicopathological characteristics and the survival probability of HCC patients were also analyzed. The results revealed that BBOX1 mRNA expression was significantly lower in HCC tissues than in noncancerous tissues. HCC patients with low BBOX1 mRNA expression had a significantly poorer overall survival than those with high BBOX1 expression did. Immunohistochemical analysis of BBOX1 protein expression further confirmed that its levels were markedly lower in HCC tissues than in paracancerous tissues. High BBOX1 expression in paracancerous tissues correlated with poor overall survival and disease-free survival in HCC patients. These findings suggest that the paracancerous BBOX1 expression might be a credible indicator for overall survival or disease-free survival in HCC patients.  \nKeywords Gamma-butyrobetaine hydroxylase 1 (BBOX1), Hepatocellular carcinoma (HCC), Paracancerous tissue, Overall survival, Disease-free survival  \nHepatocellular carcinoma (HCC) is the most common type of primary liver cancer, accounting for a significant burden of cancer-related morbidity and mortality worldwide1. Despite advancements in diagnosis and treatment modalities, the prognosis for HCC patients remains poor, primarily due to high rates of tumor recurrence and metastasis2. Therefore, there is an urgent need to identify reliable prognostic markers that can accurately predict the clinical outcomes of HCC patients.  \nMost existing HCC studies have focused on tumor tissues, whereas paracancerous tissues are usually used as controls and are rarely studied in depth. Paracancerous tissues, which refer to the non-cancerous tissues adjacent to the tumor, have emerged as critical components in tumor development and progression. It has been increasingly recognized that paracancerous tissues play active roles in facilitating tumor growth and metastasis, creating a favorable microenvironment for tumor cells3. A recent study revealed the molecular features associated with the clinical characteristics of paracancerous tissues in HCC patients and the potential role of proteins in these tissues in the immune microenvironment remodeling of tumors or tumor recurrence4. The molecular alterations that occur in paracancerous tissues have been shown to have prognostic value in various malignancies, highlighting their potential as clinically relevant biomarkers5.  \n1School of Clinical Medicine, Bengbu Medical University, Bengbu, China. 2School of Life Science, Bengbu Medical University, No. 2600 Donghai Road, Bengbu 233030, China. 3Bengbu Medical University Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, Bengbu Medical University, Bengbu, China. 4School of Basic Courses, Bengbu Medical University, Bengbu, China. 5School of Basic Medical, Bengbu Medical University, Bengbu, China.  \n6Anhui Provincial Key Laboratory of Tumor Evolution and Intelligent Diagnosis and Treatment, Bengbu Medical University, Bengbu, China. 7Laboratory Animal Center, Bengbu Medical University, Bengbu, China. 􀀍 email: [gaoyu@bbmc.edu.cn](gaoyu@bbmc.edu.cn)  \n[w","cbCaiedHoSWi5tQG","https://ap.wps.com/l/cbCaiedHoSWi5tQG","pdf",2902940,"English","# Abstract\n## Study rationale and background\n## Methods: mRNA and protein assessment\n## Key findings and survival associations\n## Biological context of BBOX1 and lipid metabolism\n## Study implications and prognostic significance","[{\"question\":\"What is the main focus of the study regarding BBOX1?\",\"answer\":\"The study evaluates whether BBOX1 expression in tumor and paracancerous tissues has clinical prognostic significance for hepatocellular carcinoma patients.\"},{\"question\":\"How were BBOX1 mRNA and protein levels assessed?\",\"answer\":\"BBOX1 mRNA expression was analyzed using bioinformatics, and BBOX1 protein levels in paired tumor and paracancerous tissues were determined by immunohistochemistry in 83 HCC patients.\"},{\"question\":\"What relationship does BBOX1 expression show with patient survival outcomes?\",\"answer\":\"Low BBOX1 mRNA expression in HCC is associated with poorer overall survival, and high BBOX1 expression in paracancerous tissues correlates with worse overall survival and disease-free survival.\"}]","High expression of BBOX1 in paracancerous tissue is associated with poor prognosis in hepatocellular carcinoma patients | PDF",1789993780]