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Using multi-region, single-cell DNA sequencing in two prostatectomy patients, the study maps somatic copy number alterations (SCNAs) across tumor-rich and normal regions. Two karyotype classes are defined: pseudodiploid cells with few SCNAs and aneuploid cells with extensive alterations. Pseudodiploid cells form many small subclones, from highly localized to broadly spread, while aneuploid cells appear throughout the prostate, including normal areas.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/high-clonal-diversity-and-spatial-genetic-admixture-in-early-prostate-cancer-and-surrounding-normal-tissue/327483/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/high-clonal-diversity-and-spatial-genetic-admixture-in-early-prostate-cancer-and-surrounding-normal-tissue/327483.png","ImageObject",300,407,{"name":92,"@type":93},"Emma Wilson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What technique is used to characterize SCNAs across prostate regions?","Question",{"text":113,"@type":114},"The study performs multi-region, single-cell DNA sequencing to profile somatic copy number alterations across tumor-rich and surrounding normal tissue.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How many karyotype classes are identified in early localized tumors?",{"text":118,"@type":114},"Two distinct karyotypes are identified: pseudodiploid cells with few SCNAs and highly aneuploid cells with extensive alterations.",{"name":120,"@type":111,"acceptedAnswer":121},"How do pseudodiploid and aneuploid cells differ in spatial distribution?",{"text":122,"@type":114},"Pseudodiploid cells form numerous small subclones with spatial patterns ranging from highly localized to broadly spread, while aneuploid cells are detected throughout the prostate, including normal regions.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},327483,1790515913,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":146,"preview_page_count":4},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Article [https://doi.org/10.1038/s41467-024-47664-z](https://doi.org/10.1038/s41467-024-47664-z)  \nHigh clonal diversity and spatial genetic admixture in early prostate cancer and surrounding normal tissue  \nReceived: 10 August 2023  \n\n| Accepted: 9 April 2024 |\n| --- |\n| |\n| Check for updates |\n\nNing Zhang1,2,14,15, Luuk Harbers 1,2,15, Michele Simonetti 1,2,15, Constantin Diekmann 1,2, Quentin Verron1,2, Enrico Berrino 3,4,  \nSara E. Bellomo3,5, Gabriel M. C. Longo 6, Michael Ratz 7, Niklas Schultz2,8, Firas Tarish9, Peng Su10, Bo Han10, Wanzhong Wang8, Sofia Onorato1,2, Dora Grassini4, Roberto Ballarino 1,2, Silvia Giordano 3,5, Qifeng Yang11, Anna Sapino 3,4, Jonas Frisén 7, Kanar Alkass7,8, Henrik Druid 8, Vassilis Roukos 6,12, Thomas Helleday 2,8, Caterina Marchiò3,4,  \nMagda Bienko 1,2,13 & Nicola Crosetto 1,2,13   \nSomatic copy number alterations (SCNAs) are pervasive in advanced human cancers, but their prevalence and spatial distribution in early-stage, localized tumors and their surrounding normal tissues are poorly characterized. Here, we perform multi-region, single-cell DNA sequencing to characterize the SCNA landscape across tumor-rich and normal tissue in two male patients with localized prostate cancer. We identify two distinct karyotypes: ‘pseudodiploid’ cells harboring few SCNAs and highly aneuploid cells. Pseudo-diploid cells form numerous small-sized subclones ranging from highly spatially localized to broadly spread subclones. In contrast, aneuploid cells do not form subclones and are detected throughout the prostate, including normal tissue regions. Highly localized pseudo-diploid subclones are confined within tumorrich regions and carry deletions in multiple tumor-suppressor genes. Our study reveals that SCNAs are widespread in normal and tumor regions across the prostate in localized prostate cancer patients and suggests that a subset of pseudo-diploid cells drive tumorigenesis in the aging prostate.  \nSomatic copy number alterations (SCNAs) are pervasive in human cancers1–3. It has been proposed that SCNAs form in the early stages of tumorigenesis in punctuated evolutionary bursts4–7. Indeed, SCNAs have been detected in pre-malignant lesions such as Barret’s  \nesophagus years before the onset of invasive esophageal cancer8 and oral leukoplakias undergoing progressive transformation to oralsquamous cell carcinomas9. However, the exact timing and mechanisms of SCNA formation during carcinogenesis remain poorly  \n1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm 17177, Sweden. 2Science for Life Laboratory, Stockholm 17177, Sweden. 3Candiolo Cancer Institute, FPO – IRCCS, Candiolo, SP142, km 3,95, 10060 Turin, Italy. 4Department of Medical Sciences, University of Turin, Turin, Italy. 5Department of Oncology, University of Turin, Turin, Italy. 6Institute of Molecular Biology(IMB), Mainz55128, Germany. 7Department of Celland Molecular Biology, Karolinska Institute, Stockholm 17177, Sweden. 8Department of Oncology and Pathology, Karolinska Institutet, Stockholm 17177, Sweden. 9Södersjukhuset, Stockholm 11883, Sweden. 10Department of Pathology, Qilu Hospital of Shandong University, Ji’nan 250012, China. 11Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Ji’nan 250012, China. 12Department of General Biology, Medical School, University of Patras, Patras, Greece. 13Human Technopole, Viale Rita Levi-Montalcini 1, 20157 Milan, Italy. 14Present address: Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, Ji’nan 250012, China. 15These authors contributed equally: Ning Zhang, Luuk Harbers, Michele Simonetti. e-mail: nicola.crosetto@ki.se  \nunderstood and a map of SCNAs in early-stage, localized tumors and their surrounding normal tissues is missing.  \nIn prostate cancers, SCNAs represent the predominant type of genomic alteration and are thought to be the major driver of these tumors10. Early-stage localized prostat","cbCaihITqWeiHA3j","https://ap.wps.com/l/cbCaihITqWeiHA3j","pdf",3172473,17,"English","# Introduction\n## SCNA prevalence and early formation\n## Spatial heterogeneity in localized prostate cancer\n## Prior spatial transcriptomics evidence\n# Methods and Approach\n## Multi-region single-cell copy number profiling\n## Targeted deep sequencing of matched regions\n# Results\n## SCNA landscape across tumor-rich and normal tissues\n## Two karyotype classes: pseudodiploid vs aneuploid\n## Subclonal spatial patterns in localized disease\n# Implications","[{\"question\":\"What technique is used to characterize SCNAs across prostate regions?\",\"answer\":\"The study performs multi-region, single-cell DNA sequencing to profile somatic copy number alterations across tumor-rich and surrounding normal tissue.\"},{\"question\":\"How many karyotype classes are identified in early localized tumors?\",\"answer\":\"Two distinct karyotypes are identified: pseudodiploid cells with few SCNAs and highly aneuploid cells with extensive alterations.\"},{\"question\":\"How do pseudodiploid and aneuploid cells differ in spatial distribution?\",\"answer\":\"Pseudodiploid cells form numerous small subclones with spatial patterns ranging from highly localized to broadly spread, while aneuploid cells are detected throughout the prostate, including normal regions.\"}]","High clonal diversity and spatial genetic admixture in early prostate cancer and surrounding normal tissue | PDF",1789977305,43]