[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-52084-en":3,"doc-seo-52084-105":30,"detail-sidebar-cat-0-en-105":91},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"doc_content":15,"file_id":16,"file_url":17,"file_type":18,"file_size":19,"view_count":20,"is_deleted":4,"is_public":21,"is_downloadable":21,"audit_status":21,"page_count":22,"language":23,"language_code":24,"site_id":25,"html_lang":24,"table_of_contents":26,"faqs":27,"seo_title":13,"seo_description":14,"update_tm":28,"read_time":29},52084,2336464648322,"Aria","https://ap-avatar.wpscdn.com/avatar/2200025388227c56fec?_k=1778556882303663488",8,"Research & Report","Handbook of Analysis of Oligonucleotides and Related Products Jose","Handbook of Analysis of Oligonucleotides and Related Products provides a structured reference for characterizing oligonucleotides and related materials. The contents outline analytical workflows for purity and impurities using reversed-phase and AEX HPLC, size-exclusion HPLC for molecular weight, and LC–mass spectrometry for identification. Additional chapters cover sequence determination with MS/MS and MALDI-TOF, Tm analysis, electrophoretic purity and content, immunoassay-based bioanalysis, potency and endotoxin testing, residual solvent analysis, and key physicochemical characterization methods including NMR, IR, stability-indicating approaches, and HILIC.","Handbook of Analysis of Oligonucleotides and  \nRelated Products  \nE d it E d by  \nJ o s e V. B o n i l l a  G. s usa n s r i Vat sa  \nHandbook of Analysis of Oligonucleotides and  \nRelated Products  \nE d it E d by  \nJ o s e V. B o n i l l a  \nGirindus America, Cincinnati, Ohio, USA  \nG. s usa n s r i Vat sa  \nElixinPharma, Encinitas, California, USA  \nBoca Raton London New York  \nCRC Press is an imprint of the  \nTaylor & Francis Group, an informa business  \nCRC Press  \nTaylor & Francis Group  \n6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742  \n© 2011 by Taylor and Francis Group, LLC  \nCRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works  \nPrinted in the United States of America on acid-free paper 10 9 8 7 6 5 4 3 2 1  \nInternational Standard Book Number-13: 978-1-4398-1994-4 (Ebook-PDF)  \nThis book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint.  \nExcept as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, microfilming, and recording, or in any information storage or retrieval system, without written permission from the publishers.  \nFor permission to photocopy or use material electronically from this work, please [access www.copyright.com](access www.copyright.com) ([http://](http://)[ ](http://)[www.copyright.com/](www.copyright.com/)) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of users. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged.  \nTrademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe.  \nVisit the Taylor & Francis Web site at  \n[http://www.taylorandfrancis.com](http://www.taylorandfrancis.com)  \nand the CRC Press Web site at  \n[http://www.crcpress.com](http://www.crcpress.com)  \nContents  \nPreface................................................................................................................................................ v  \nEditors ..............................................................................................................................................vii  \nContributors ......................................................................................................................................ix  \nIntroduction.......................................................................................................................................xi  \nChapter 1 Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography ................................................................ 1  \nHagen Cramer, Kevin J. Finn, and Eric Herzberg  \nChapter 2 Purity Analysis and Impurities Determination by AEX-HPLC ................................47  \nJim Thayer, Veeravagu Murugaiah, and Yansheng Wu  \nChapter 3 Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC...... 105  \nMing Fai Chan and Ipsita Roymoulik  \nChapter 4 Analysis of Oligonucleotides by Liquid Chromatography and Mass Spe","cbCaiaqwGpoNAEoU","https://ap.wps.com/l/cbCaiaqwGpoNAEoU","pdf",15637755,2,1,504,"English","en",105,"# Preface\n# Introduction\n# Purity analysis and impurities determination by reversed-phase HPLC\n# Purity analysis and impurities determination by AEX-HPLC\n# Purity analysis and molecular weight determination by size exclusion HPLC\n# Analysis by liquid chromatography and mass spectrometry\n# Sequence determination and confirmation by MS/MS and MALDI-TOF\n# Tm analysis of oligonucleotides\n# Purity and content analysis by capillary gel electrophoresis\n# Bioanalysis of therapeutic oligonucleotides using hybridization-based immunoassay\n# Oligonucleotide assay and potency\n# Microbial analysis: endotoxin testing\n# Analysis of residual solvents by headspace gas chromatography\n# Determination of extinction coefficient\n# Structural determination by NMR\n# Infrared analysis of oligonucleotides\n# Stability indicating methods for oligonucleotide products\n# Analysis by hydrophilic interaction chromatography","[{\"question\":\"What analytical techniques are used to determine oligonucleotide purity and impurities?\",\"answer\":\"Purity and impurities are addressed using reversed-phase high-performance liquid chromatography and AEX-HPLC, with additional separation approaches such as size exclusion HPLC and capillary gel electrophoresis.\"},{\"question\":\"How does the handbook support oligonucleotide sequence determination and confirmation?\",\"answer\":\"Sequence determination and confirmation are covered using MS/MS and MALDI-TOF methods.\"},{\"question\":\"Which methods are described for physicochemical and stability-related characterization?\",\"answer\":\"The contents include Tm analysis, determination of extinction coefficient, structural determination by NMR, infrared analysis, and stability-indicating methods for oligonucleotide products.\"}]",1783638348,1270,{"code":4,"msg":31,"data":32},"ok",{"site_id":25,"language":24,"slug":33,"title":13,"keywords":34,"description":14,"schema_data":35,"social_meta":86,"head_meta":88,"extra_data":90,"updated_unix":28},"handbook-of-analysis-of-oligonucleotides-and-related-products-jose","",{"@graph":36,"@context":85},[37,53,68],{"@type":38,"itemListElement":39},"BreadcrumbList",[40,44,47,50],{"item":41,"name":42,"@type":43,"position":21},"https://docshare.wps.com","Home","ListItem",{"item":45,"name":46,"@type":43,"position":20},"https://docshare.wps.com/document/","Document",{"item":48,"name":12,"@type":43,"position":49},"https://docshare.wps.com/document/research-report/",3,{"item":51,"name":13,"@type":43,"position":52},"https://docshare.wps.com/document/handbook-of-analysis-of-oligonucleotides-and-related-products-jose/52084/",4,{"url":51,"name":13,"@type":54,"author":55,"headline":13,"publisher":57,"fileFormat":60,"inLanguage":24,"description":14,"dateModified":61,"datePublished":62,"encodingFormat":60,"isAccessibleForFree":63,"interactionStatistic":64},"DigitalDocument",{"name":9,"@type":56},"Person",{"url":41,"name":58,"@type":59},"DocShare","Organization","application/pdf","2026-07-12","2026-07-09",true,{"@type":65,"interactionType":66,"userInteractionCount":20},"InteractionCounter",{"@type":67},"ViewAction",{"@type":69,"mainEntity":70},"FAQPage",[71,77,81],{"name":72,"@type":73,"acceptedAnswer":74},"What analytical techniques are used to determine oligonucleotide purity and impurities?","Question",{"text":75,"@type":76},"Purity and impurities are addressed using reversed-phase high-performance liquid chromatography and AEX-HPLC, with additional separation approaches such as size exclusion HPLC and capillary gel electrophoresis.","Answer",{"name":78,"@type":73,"acceptedAnswer":79},"How does the handbook support oligonucleotide sequence determination and confirmation?",{"text":80,"@type":76},"Sequence determination and confirmation are covered using MS/MS and MALDI-TOF methods.",{"name":82,"@type":73,"acceptedAnswer":83},"Which methods are described for physicochemical and stability-related characterization?",{"text":84,"@type":76},"The contents include Tm analysis, determination of extinction coefficient, structural determination by NMR, infrared analysis, and stability-indicating methods for oligonucleotide 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