[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-628493-105":59,"doc-detail-628493-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","green-synthesis-of-silver-nanoparticles-from-cotoneaster-lacteus-experimental-characterization-and-mechanistic-insights","Green Synthesis of Silver Nanoparticles from Cotoneaster lacteus - Experimental Characterization and Mechanistic Insights","","Plant-mediated green synthesis of silver nanoparticles uses Cotoneaster lacteus extract to produce and comprehensively characterize AgNPs. Extract phytochemical profiling by GC-MS/MS and LC-ESI-MS/MS identifies linoleic acid and trans-ferulic acid as key constituents. AgNPs are analyzed via UV-Vis, FT-IR, XRD, SEM/EDX, STEM, and zeta potential, revealing FCC crystallinity and predominantly spherical particles. Biological assays show stronger antibacterial and antibiofilm effects than crude extract and AgNO3, while DPPH, ABTS, and FRAP confirm enhanced antioxidant activity. DFT and molecular docking indicate oxygen-containing functional groups in trans-ferulic acid and hesperidin support formation and stabilization and interact favorably with antioxidant/antibacterial target proteins.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/green-synthesis-of-silver-nanoparticles-from-cotoneaster-lacteus-experimental-characterization-and-mechanistic-insights/628493/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/green-synthesis-of-silver-nanoparticles-from-cotoneaster-lacteus-experimental-characterization-and-mechanistic-insights/628493.png","ImageObject",300,407,{"name":92,"@type":93},"Emma Wilson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-10","2026-10-21",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Which plant material and synthesis approach were used to produce the silver nanoparticles?","Question",{"text":112,"@type":113},"Silver nanoparticles were green synthesized using Cotoneaster lacteus extract as the biological reducing and stabilizing resource.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were the nanoparticles characterized experimentally?",{"text":117,"@type":113},"The AgNPs were characterized using UV-Vis, FT-IR, XRD, SEM/EDX, STEM, and zeta potential measurements, including XRD for crystal structure and STEM for particle morphology and size.",{"name":119,"@type":110,"acceptedAnswer":120},"What do the biological assays and computational studies suggest about performance and mechanism?",{"text":121,"@type":113},"Biological assays show higher antibacterial activity and antibiofilm inhibition (>50%) and stronger antioxidant effects (DPPH, ABTS, FRAP). DFT and molecular docking indicate that trans-ferulic acid and hesperidin interact through oxygen-containing groups with silver atoms/complexes, supporting nanoparticle formation, stabilization, and target protein interactions.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},628493,1792656729,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},3848291630094,"https://eur-avatar.wpscdn.com/davatar_085a072bc5b1113ac321206ff7593b45","Applied Biochemistry and Biotechnology [https://doi.org/10.1007/s12010-026-05848-x](https://doi.org/10.1007/s12010-026-05848-x)  \nORIGINAL ARTICLE  \nGreen Synthesis of Silver Nanoparticles from Cotoneaster lacteus: Experimental Characterization and Mechanistic Insights from Biological Assays, DFT and Molecular Docking Studies  \nYunus Başar1 · AybekYiğit2 · SemihaYenigun3 · Dilara Öztürk4 · Ayşe Karacalı Tunç5 · Mesut Gök6 · Özgen Ömür Pekel7 · Ahmet ZaferTel8  \nAccepted: 15 July 2026  \n© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2026  \nAbstract  \nIn this study, silver nanoparticles were green synthesized using Cotoneaster lacteus and characterized for the first time in a comprehensive way. The phytochemical composition of the extract was analyzed by GC-MS/MS and LC-ESI-MS/MS, and the main constituents were identified as linoleic acid (63.56%) and trans-ferulic acid (55.94 ng/mL) . The C. lacteus extract-based AgNPs were characterized using UV-Vis, FT-IR, XRD, SEMEDX, STEM, and zeta potential analyses. XRD results showed a face-centered cubic crystal structure with an average crystallite size of 25.42 nm, while STEM images indicated predominantly spherical particles with an average size of 20.75 ± 3.79 nm. In the biological assays, C. lacteus extract-based AgNPs showed noticeably higher antibacterial activity compared to both the crude extract and AgNO₃ . Antibiofilm inhibition rates also exceeded 50% . Similarly, antioxidant assays (DPPH ˙ , ABTS˙⁺, and FRAP) confirmed stronger radical scavenging and reducing ability of the AgNPs compared to the extract. DFT calculations suggest that trans-ferulic acid and hesperidin interact with silver atoms through oxygen-containing functional groups, which likely contribute to nanoparticle formation and stabilization. Molecular docking results further showed favorable interactions between ferulic acid, hesperidin, and Ag complexes with key antioxidant and antibacterial target proteins, supporting the experimental findings. Overall, plant-mediated AgNPs appear promising for biomedical and biotechnological applications due to their antibacterial, antibiofilm, and antioxidant properties.  \nExtended author information available on the last page of the article  \nGraphical Abstract  \nHighlights  \n● First report of green synthesis of AgNPs using Cotoneaster lacteus extract  \n● Formation of spherical FCC-structured AgNPs (20–25 nm) confirmed  \n● Moderate antibacterial activity (MIC 128 µg/mL)  \n● Significant antibiofilm inhibition (>50%) in all tested strains  \n● Enhanced antioxidant capacity after nanoparticle formation  \n● Strong metal–ligand interactions by DFT and docking analyses  \nKeywords Cotoneaster lacteus · Antimicrobial activity · Antioxidant activity · Green Synthesis · Molecular Docking  \nAbbreviations  \nand Terminology  \nABTS 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) AgNPs Silver nanoparticles  \nBHI Brain Heart Infusion  \nDFT Density Functional Theory  \nDPPH 2,2-Diphenyl-1-picrylhydrazyl  \nEDX Energy Dispersive X-ray  \nFRAP Ferric Reducing Antioxidant Power  \nFT-IR Fourier Transform Infrared Spectroscopy  \nHOMO Highest Occupied Molecular Orbital  \nLC-ESI-MS/MS  \nLUMO MBC  \nMEP  \nMIC  \nMLCT  \nSEM  \nSPR  \nSTEM  \nTD-DFT  \nLiquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry  \nLowest Unoccupied Molecular Orbital Minimum Bactericidal Concentration Molecular Electrostatic Potential Minimum Inhibitory Concentration Metal-to-Ligand Charge Transfer Scanning Electron Microscopy Surface Plasmon Resonance  \nScanning Transmission Electron Microscopy Time-Dependent Density Functional Theory  \nIntroduction  \nNanotechnology has found widespread applications in fields such as environmental remediation, pharmaceuticals, cancer treatments, and antimicrobial technologies [1]. Alongside the development of nanomaterials, increasing importance is being placed on environmentally friendly production methods. Green syn","cbCaifTitagFQQEU","https://ap.wps.com/l/cbCaifTitagFQQEU","pdf",3985857,34,"English","# Abstract\n## Highlights\n## Keywords\n## Abbreviations and Terminology\n## Introduction","[{\"question\":\"Which plant material and synthesis approach were used to produce the silver nanoparticles?\",\"answer\":\"Silver nanoparticles were green synthesized using Cotoneaster lacteus extract as the biological reducing and stabilizing resource.\"},{\"question\":\"How were the nanoparticles characterized experimentally?\",\"answer\":\"The AgNPs were characterized using UV-Vis, FT-IR, XRD, SEM/EDX, STEM, and zeta potential measurements, including XRD for crystal structure and STEM for particle morphology and size.\"},{\"question\":\"What do the biological assays and computational studies suggest about performance and mechanism?\",\"answer\":\"Biological assays show higher antibacterial activity and antibiofilm inhibition (\\u003e50%) and stronger antioxidant effects (DPPH, ABTS, FRAP). DFT and molecular docking indicate that trans-ferulic acid and hesperidin interact through oxygen-containing groups with silver atoms/complexes, supporting nanoparticle formation, stabilization, and target protein interactions.\"}]","Green Synthesis of Silver Nanoparticles from Cotoneaster lacteus - Experimental Characterization and Mechanistic Insights | PDF",1792616780,86]