[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-329312-105":59,"doc-detail-329312-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","genome-wide-dna-methylation-profiling-reveals-a-novel-hypermethylated-biomarker-prkcb-in-gastric-cancer","Genome-wide DNA methylation profiling reveals a novel hypermethylated biomarker PRKCB in gastric cancer","","Genome-wide DNA methylation profiling was performed to identify diagnostic epigenetic biomarkers in gastric cancer. The study analyzed paired gastric cancer tissues and adjacent non-cancerous counterparts using the Illumina 935 K BeadChip, followed by bisulfite-pyrosequencing quantification and validation with pyrosequencing, immunohistochemistry, and qRT-PCR. DNA methylome analysis found 4432 differentially methylated probes, with PRKCB showing the strongest methylation disparity and promoter CpG island hypermethylation, inversely correlated with mRNA expression. PRKCB mRNA and protein levels were reduced in gastric cancers across TCGA and GEO.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/genome-wide-dna-methylation-profiling-reveals-a-novel-hypermethylated-biomarker-prkcb-in-gastric-cancer/329312/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/genome-wide-dna-methylation-profiling-reveals-a-novel-hypermethylated-biomarker-prkcb-in-gastric-cancer/329312.png","ImageObject",300,407,{"name":92,"@type":93},"Eden","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-27","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main goal of this study?","Question",{"text":112,"@type":113},"To characterize genome-wide DNA methylation patterns in gastric cancer and identify potential diagnostic epigenetic biomarkers, focusing on PRKCB as a hypermethylated candidate.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers measure DNA methylation?",{"text":117,"@type":113},"They used the Illumina 935 K BeadChip for genome-wide methylation profiling, with bisulfite-pyrosequencing to quantify methylation levels of methylated genes and specific CpG sites.",{"name":119,"@type":110,"acceptedAnswer":120},"What key finding involved PRKCB in gastric cancer?",{"text":121,"@type":113},"PRKCB showed the most prominent DNA methylation disparity, with promoter CpG island hypermethylation; PRKCB methylation correlated inversely with mRNA expression and PRKCB mRNA/protein were reduced in gastric cancers.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},329312,1790130110,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},1374391974468,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nGenome-wide DNA methylation profiling reveals a novel hypermethylated biomarker PRKCBin gastric cancer  \nLeyan Li1,3, Xiao Fei1,3, Huan Wang1,2, Sihai Chen1, XinboXu1, Huajing Ke1, Yanan Zhou1, Yi Hu1, Cong He1, Chuan Xie1, Nonghua Lu1, Jianping Liu1, Yin Zhu1􀀍 & Nianshuang Li1􀀍  \nGlobally, gastric cancer (GC) ranks among the most prevalent forms of malignancy, posing a significant health burden. Epigenetic modifications, predominantly characterized by alterationsin DNA methylation patterns, have been linked to a diverse array of neoplastic processes. Here, we undertake a comprehensive analysis of the DNA methylation signature in GC, with the aim to discover the potential diagnostic epigenetic biomarkers. Utilizing the Illumina 935 K BeadChip, we conducted a genome-wide exploration of DNA methylation patterns in four paired samples of GC tissues and adjacent non-cancerous counterparts. The bisulfite-pyrosequencing (n = 7) was employed to the quantification for methylated gene. The pubic databases including GWAS Catalog, TCGA and GEO were used. The immunohistochemistry and qRT-PCR analysis were performed. In contrast to adjacent tissues, GC tissues manifested pronounced hypermethylation patterns specifically within the promoter cytosine-phosphate-guanine (CpG) islands, indicating localized epigenetic alterations. DNA methylome analysis further revealed 4432 differentially-methylated probes (DMPs), with the gene PRKCB exhibited the most prominent average DNA methylation disparity (mean Δβ = 0.353) . Pyrosequencing validation confirmed three DMPs within the PRKCB promoter (cg08406370, cg00735962, and cg18526361). Notably, the mean methylation levels of PRKCB were inversely correlated with mRNA expression levels in the GWAS Catalog. Furthermore, both mRNA and protein expression levels of PRKCB were significantly reduced inGCs when compared to their adjacent non-cancerous counterparts, verified by TCGA and GEO database. Our study reveals significant DNA methylation alterations inGC and emphasizes the pivotal role of PRKCB gene hypermethylationin conferring GC risk, which offers fresh perspectives for advancing diagnostic approaches and therapeutic strategies for GC.  \nKeywords Genome-wide DNA methylation, Gastric cancer, PRKCB  \nGastric cancer (GC) stands as a formidable malignancy, occupying the fifth position among the primary causes of cancer-associated mortality worldwide. Its rapid progression into advanced stages, coupled with a high propensity for metastasis, contributes significantly to its devastating impact1,2. Despite great achievements in diagnostic and therapeutic methods, the prognosis of GC patients remains disappointing3. Delayed diagnosis and the absence of efficient therapies contribute to the high mortality rate of GC patients4. The exploration of epigenomic regulation has reinforced the potential for utilizing epigenetic drugs to treat solid tumors5. Furthermore, understanding the regulation of gene expression may aid doctors in determining which patients are most likely to benefit from epigenetic drug therapies.  \nDNA methylation, particularly targeting promoter-associated cytosine-phosphate-guanine (CpG) islands, is recognized as a pivotal epigenetic modification that exerts significant influence on the regulation of gene expression6. The methylation profile of cancer is unique, characterized by overall hypomethylated DNA and hypermethylated CpG islands7. This hypermethylation in promoter CpG islands and the simultaneous  \n1Department of Gastroenterology, Jiangxi Provincial Key Laboratory of Digestive Diseases, Jiangxi Clinical Research Center for Gastroenterology, Digestive Disease Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China. 2Postdoctoral Innovation Practice Base, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China. 3These","cbCaik2lr9pyUY9C","https://ap.wps.com/l/cbCaik2lr9pyUY9C","pdf",2590897,"English","# Introduction\n# DNA methylation and study rationale\n# Methods and experimental design\n# Study population and eligibility\n# Results and validation highlights","[{\"question\":\"What was the main goal of this study?\",\"answer\":\"To characterize genome-wide DNA methylation patterns in gastric cancer and identify potential diagnostic epigenetic biomarkers, focusing on PRKCB as a hypermethylated candidate.\"},{\"question\":\"How did the researchers measure DNA methylation?\",\"answer\":\"They used the Illumina 935 K BeadChip for genome-wide methylation profiling, with bisulfite-pyrosequencing to quantify methylation levels of methylated genes and specific CpG sites.\"},{\"question\":\"What key finding involved PRKCB in gastric cancer?\",\"answer\":\"PRKCB showed the most prominent DNA methylation disparity, with promoter CpG island hypermethylation; PRKCB methylation correlated inversely with mRNA expression and PRKCB mRNA/protein were reduced in gastric cancers.\"}]","Genome-wide DNA methylation profiling reveals a novel hypermethylated biomarker PRKCB in gastric cancer | PDF",1789983590,25]