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This research employs Sequence-Related Amplified Polymorphism (SRAP) molecular markers to evaluate banana genetic diversity, identify suitable SRAP primer combinations, and assess similarity relationships among banana accessions. Genomic DNA is extracted using DNeasy Plant Mini Kit and amplified with selected SRAP primers. Analysis using similarity index values and NTSYS 2.0 dendrograms shows 204 loci from nine primer combinations and low diversity among 19 cultivars (0–0.27), clustering both cultivars and wild samples into two major groups (0.70–0.97).",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/genetic-diversity-of-bananas-musa-spp-in-pesawaran-district-lampung-province-based-on-sequence-related-amplified-polymorphism-srap-marker/274742/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/genetic-diversity-of-bananas-musa-spp-in-pesawaran-district-lampung-province-based-on-sequence-related-amplified-polymorphism-srap-marker/274742.png","ImageObject",300,407,{"name":92,"@type":93},"Nguyễn Văn Học","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-11","2026-09-15",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",15,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the purpose of using SRAP markers in this study?","Question",{"text":113,"@type":114},"To determine banana genetic diversity, select suitable SRAP primer combinations, and evaluate similarity relationships among bananas in Pesawaran District.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was banana genomic DNA obtained and amplified?",{"text":118,"@type":114},"Total genomic DNA was extracted using DNeasy Plant Mini Kit according to the manufacturer’s protocol, then amplified using SRAP primers.",{"name":120,"@type":111,"acceptedAnswer":121},"What do the results indicate about genetic diversity and clustering?",{"text":122,"@type":114},"Nineteen banana cultivars show low genetic diversity values (0–0.27), and SRAP markers group 19 cultivars and 4 wild banana samples into two major clusters with similarity coefficients ranging from 0.70 to 0.97.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},274742,1789482469,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":130,"read_time":145,"preview_page_count":4},1374402739827,"https://ap-avatar.wpscdn.com/avatar/14000c97e7351f1a627?x-image-process=image/resize,m_fixed,w_180,h_180&k=1787885694763230660","Original Research Paper  \nGenetic Diversity of Bananas (Musa spp.) in Pesawaran District, Lampung Province Based on Sequence Related Amplified Polymorphism (SRAP) Marker  \nLeni Agustin1*, Eti Ernawiati1*, Lulut Dwi Sulistyaningsih2*, Mahfut1  \n1Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung, Lampung, Indonesia;  \n2Pusat Riset Biosistematika dan Evolusi, Badan Riset dan Inovasi Nasional, Indonesia;  \nArticle History  \nReceived : August 07th, 2023  \nRevised : August 31th, 2023  \nAccepted : October 15th, 2023  \n*Corresponding Author:  \nLeni Agustin, Eti Ernawiati, Lulut Dwi Sulistyaningsih,  \nProgram Studi Biologi, Lampung, Indonesia.  \nPusat Riset Biosistematika dan Evolusi, Badan Riset dan Inovasi Nasional, Indonesia; Email:  \n[leniagustin714@gmail.com](leniagustin714@gmail.com)[etiernawiat@fmipa.unila.ac.id](etiernawiat@fmipa.unila.ac.id)[ ](etiernawiat@fmipa.unila.ac.id)[lulutjv@gmail.com](lulutjv@gmail.com)  \nAbstract: Banana (Musa spp.) is a leading commodity that makes a major contribution to national fruit production. One of the districts in Lampung Province which has a significant contribution to Indonesia's banana production is Pesawaran Regency. The molecular marker used in this study is Sequence-Related Amplified Polymorphism (SRAP) . This study aims to determine the genetic diversity of bananas, determine the suitable combination of SRAP primers to determine the genetic diversity of bananas and determine the similarity relationship of bananas in the Pesawaran District. The total genomic DNA was extracted using DNeasy Plant Mini Kit (Geneaid) according to the protocol from the manufacturer followed by their amplification using SRAP primers. Similarity analysis was carried out based on similarity index values and dendrogram construction using the NTSYS 2.0 program. Research results show that a total of 204 loci (167 polymorphic loci and 37 monomorphic loci) were obtained from 9 selected primer combinations. The genetic diversity of 19 banana cultivars originating from Pesawaran Regency resulted in low genetic diversity values (0-0.27) . Banana kinship based on Sequence Related Amplified Polymorphism (SRAP) markers divided 19 banana cultivar samples and 4 wild banana samples into 2 large clusters with similarity coefficient values between 0.70-0.97.  \nKeywords: Bananas, genetic diversity, Lampung, Pesawaran, SRAP.  \nPendahuluan  \nPisang (Musa spp.) salah satu buah tropis yang paling populer di Indonesia. Pisang dapatdikonsumsi langsung sebagai buah atau dapat diolah menjadi makanan lain. Pisang yang dibudidayakan sebagian besar telah berevolusidari 2 spesies pisang liar (Musa balbisiana Colla dan Musa acuminata Colla), masingmasing mendonor genom A dan B pada pisang budidaya (Simmonds 1962) . Upaya pemuliaansaat ini untuk perbaikan pisang bergantung padaintrogresi gen-gen yang berguna dari progenitor diploid liar dan budidaya (Ortiz & Vuylsteke 1994) . Oleh karena itu perlu dilakukan kajiankeragaman genetik dan hubungan antara aksesi diploid liar dan budidaya. Provinsi Lampung  \nadalah sentra produksi pisang di Indonesia. Salah satu kabupaten di Provinsi Lampung dengan produktivitas pisang yang relatif tinggiadalah Kabupaten Pesawaran.  \nMenurut data Badan Pusat Statistika (2022), sepanjang tahun 2022 Indonesia mampu memproduksi pisang sebanyak 9,24 juta ton, meningkat dari tahun sebelumnya, yaitu sebesar 8,74 juta ton pada tahun 2021. Badan Pusat Statistika menginformasikan bahwa produksi pisang terus meningkat selam 5 tahun terkahirdengan peningkatan 5,2% per tahun. Provinsi Lampung menduduki posisi ketiga yang memberikan kontribusi sebesar 1,22 juta ton terhadap produksi pisang nasional tahun 2022. Kontirbusi provinsi Jawa Timur sebesar 2,62 juta ton dan Jawa barat sebesar 1,31 juta ton  \nAgustin et al.,(2023). Jurnal Biologi Tropis, 23 (4): 680 – 690  \nDOI: [http://dx.doi.org/10.29303/jbt.v23i4.5493](http://dx.doi.org/10.29303/jbt.v23i4.5493)  \n(BPS, 2022) . Besarnya kontri","cbCaiqL90ZRvzvsi","https://ap.wps.com/l/cbCaiqL90ZRvzvsi","pdf",510906,11,"English","# Article History\n# Abstract\n# Keywords\n# Pendahuluan\n## Pisang sebagai komoditas tropis di Indonesia\n## Kebutuhan analisis keragaman genetik\n## Pendekatan morfologi dan molekuler\n## Penanda molekuler dan peran SRAP","[{\"question\":\"What is the purpose of using SRAP markers in this study?\",\"answer\":\"To determine banana genetic diversity, select suitable SRAP primer combinations, and evaluate similarity relationships among bananas in Pesawaran District.\"},{\"question\":\"How was banana genomic DNA obtained and amplified?\",\"answer\":\"Total genomic DNA was extracted using DNeasy Plant Mini Kit according to the manufacturer’s protocol, then amplified using SRAP primers.\"},{\"question\":\"What do the results indicate about genetic diversity and clustering?\",\"answer\":\"Nineteen banana cultivars show low genetic diversity values (0–0.27), and SRAP markers group 19 cultivars and 4 wild banana samples into two major clusters with similarity coefficients ranging from 0.70 to 0.97.\"}]","Genetic Diversity of Bananas (Musa spp.) in Pesawaran District, Lampung Province Based on Sequence Related Amplified Polymorphism (SRAP) Marker | PDF",28]