[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-510987-105":59,"doc-detail-510987-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","gator-anti-human-igg-fc-gen-ii-hfcii-probes-product-insert","Gator - Anti-Human IgG Fc Gen II (HFCII) Probes - Product Insert","","Gator Anti-Human IgG Fc Gen II (HFCII) probes enable detection, quantification, and characterization of binding kinetics and affinities for human IgG antibodies (subclasses 1–4), recombinant human IgG, and human IgG-derived Fc-fused proteins. The probes specifically bind the Fc region with similar affinity across all four subclasses and show no cross-reactivity to other species’ IgGs. They support assays with crude samples and offer flexibility for epitope binning, high-throughput workflows, process development, isotyping of hybridoma cell lysates, and hit-to-lead antibody discovery.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/gator-anti-human-igg-fc-gen-ii-hfcii-probes-product-insert/510987/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/gator-anti-human-igg-fc-gen-ii-hfcii-probes-product-insert/510987.png","ImageObject",300,407,{"name":92,"@type":93},"awa","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-11","2026-10-06",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What targets do the HFCII probes recognize, and what binding specificity do they offer?","Question",{"text":112,"@type":113},"HFCII probes bind the Fc region of human IgG antibodies, covering IgG subclasses 1–4, recombinant human IgG, and human IgG-derived Fc-fused proteins. They show similar affinities across all four subclasses and do not cross-react with IgGs from other species.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"What dynamic range and limit of detection (LoD) are reported for quantification?",{"text":117,"@type":113},"The application summary reports a dynamic range of 0.3–6000 µg/mL at 400 rpm for 60 seconds and a LoD of 0.1 µg/mL at 1000 rpm for 300 seconds.",{"name":119,"@type":110,"acceptedAnswer":120},"How many times can the probes be regenerated, and what are the regeneration conditions?",{"text":121,"@type":113},"The probes can be regenerated up to 20 times in both Q and K assays. Regeneration uses Regen Buffer (No Salt) with neutralization buffer, performed at 1000 rpm for 5 seconds each across a total of three cycles.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},510987,1792221100,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":81,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":39,"preview_page_count":4},3985747858093,"https://ap-avatar.wpscdn.com/davatar_3d24733baf745e90a7e4bdd5f77d97b2","PRODUCT INSERT  \nGator® Anti-Human IgG Fc Gen II (HFCII) Probes Part No. 160024  \nOverview  \nGator® Anti-Human IgG Fc Gen II (HFCII) probes can detect, quantitate, and characterize the kinetics affinities of all human IgG antibodies (subclasses 1-4), recombinant human IgG antibodies and human IgG-derived Fc fused proteins. HFCII specifically binds to Fc region of human IgG antibodies and has similar binding affinities to all four subclasses of human IgG, without cross-reacting to IgGs of other species. HFCII is compatible with crude samples. Its ease and versatility make it useful in epitopebinning, high-throughput applications, process development, isotyping of crude hybridoma, cell lysates, and hit-to-lead antibody discovery.  \nApplication Summary  \n• Dynamic range: 0.3 - 6000 µg/mL at 400 rpm, 60 sec  \n• LoD: 0.1 µg/mL at 1000 rpm, 300 sec  \n• Precision/accuracy: \u003C 10% CVs  \n• Regeneration: Up to 20 times in both Q and K assays  \n• Throughput: 8 samples in 2 min, 96 samples in 34 min  \nMaterials Required  \n\n| Max Plate | Part No. 130062 |\n| --- | --- |\n| Gator BLI Plate | Part No. 130260 |\n| Q Buffer | Part No.120010 |\n| K Buffer | Part No. 120011 |\n| Regen Buffer (No Salt) | Part No. 120063 |\n\nStorage  \nStore HFCII probes (Part Number:160024) at room temperature in a foil pouch, ensuring zipper is fully sealed to avoid humidity/ moisture contamination. In high-humidity environments, storage inside a dry cabinet is recommended.  \nGeneral Methods  \nSample Volume  \n• Black Plate (96-well plate): 200 µL (180 µL minimum)  \n• Black Plate (384-well plate): 100 µL (80 µL minimum)  \n• Max Plate: 250 µL (280 µL maximum)  \nPre-wet Conditions  \n• Use the same buffer or media as the samples being quantified are in to minimize background response due to nonspecific binding.  \n• 10 min at 1000 rpm when using buffers  \n• 30 min at 1000 rpm when using media  \nRegeneration  \n• Regenerate in Regen Buffer (No Salt) (PN: 120063) and neutralization buffer (buffer diluent) at 1000 rpm for 5 sec each, for a total of three cycles.  \n• For optimal performance, regenerate the probes once before starting the assay.  \n• The probes can be regenerated up to 20 times without showing significant loss of binding to human IgG Fc region.  \nGator Bio, Inc. 2455 Faber Place, Palo Alto, CA 94303, USA • +1 855 208 0743 • [info@gatorbio.com](info@gatorbio.com) • [GatorBio.com](GatorBio.com)[ ](GatorBio.com)© 2022 Gator Bio, Inc. All rights reserved. Gator is a registered trademark of Gator Bio, Inc. PI024_6-2022  \nPRODUCT INSERT  \nGator® Anti-Human IgG Fc Gen II (HFCII) Probes Part No. 160024  \nQuantification Assay  \nFigure 1: Quantification of human IgG in Q buffer over 20 regeneration cycles. IgG concentration ranges between 0.3-6000 µg/mL. The data was acquired in 60 sec at 400 rpm.  \nSample and Standard Preparation  \n• Quantify purified IgG and subtypes in Q buffer preferably.  \n• Prepare IgG standards in the same buffer or media that the samples are in. Use the same IgG isotype that is to be quantified in the samples.  \n• Dilute crude samples with Q buffer or pre-wet the probes in same media as the samples for at least 30 min and regenerate 4-5 times to stabilize the surface.  \n• Test several dilutions (1:2, 1:3, 1:4) of the samples to ensure that IgG concentration lies within the dynamic range of the probe.  \nTips for Quantitation Assays  \n• Run a buffer diluent only as a reference for background subtraction during data analysis.  \n• Standard curves can be saved and used for subsequent experiments, if sample matrix is same.  \n• The concentration of sample(s) being analyzed should fall within the concentration range of the standard curve for an accurate quantification.  \nTable 1: Average binding rates of human IgG, calculated concentrations and their respective % CV after 20 regeneration cycles.  \nGator Bio, Inc. 2455 Faber Place, Palo Alto, CA 94303, USA • +1 855 208 0743 • [info@gatorbio.com](info@gatorbio.com) • [GatorBio.com](GatorBio.com)[ ](GatorBio.co","cbCailaraM0Fpv47","https://ap.wps.com/l/cbCailaraM0Fpv47","pdf",975513,"English","# Overview\n# Application Summary\n# Materials Required\n# Storage\n# General Methods\n## Sample Volume\n## Pre-wet Conditions\n## Regeneration\n# Quantification Assay\n## Sample and Standard Preparation\n## Tips for Quantitation Assays\n# Kinetics Assay\n## Tips for Kinetics Assays\n## Sample Preparation\n# Ordering Information","[{\"question\":\"What targets do the HFCII probes recognize, and what binding specificity do they offer?\",\"answer\":\"HFCII probes bind the Fc region of human IgG antibodies, covering IgG subclasses 1–4, recombinant human IgG, and human IgG-derived Fc-fused proteins. They show similar affinities across all four subclasses and do not cross-react with IgGs from other species.\"},{\"question\":\"What dynamic range and limit of detection (LoD) are reported for quantification?\",\"answer\":\"The application summary reports a dynamic range of 0.3–6000 µg/mL at 400 rpm for 60 seconds and a LoD of 0.1 µg/mL at 1000 rpm for 300 seconds.\"},{\"question\":\"How many times can the probes be regenerated, and what are the regeneration conditions?\",\"answer\":\"The probes can be regenerated up to 20 times in both Q and K assays. Regeneration uses Regen Buffer (No Salt) with neutralization buffer, performed at 1000 rpm for 5 seconds each across a total of three cycles.\"}]","Gator - Anti-Human IgG Fc Gen II (HFCII) Probes - Product Insert | PDF",1791246827]