[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-340817-105":59,"doc-detail-340817-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","galangin-promotes-apoptosis-by-upregulating-the-pro-apoptotic-gene-bax-in-triple-negative-breast-cancer","Galangin promotes apoptosis by upregulating the pro-apoptotic gene BAX in triple-negative breast cancer","","Triple-negative breast cancer (TNBC) is an aggressive, targeted-therapy–deficient subtype with poor prognosis and substantial treatment side effects. This study evaluates Galangin, a naturally occurring flavonoid, as a therapeutic agent for TNBC. A dual in silico and in vitro approach is applied, combining network pharmacology predictions with experiments including MTT, wound healing, apoptosis, ROS, mitochondrial potential assays, and RT-PCR.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/galangin-promotes-apoptosis-by-upregulating-the-pro-apoptotic-gene-bax-in-triple-negative-breast-cancer/340817/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/galangin-promotes-apoptosis-by-upregulating-the-pro-apoptotic-gene-bax-in-triple-negative-breast-cancer/340817.png","ImageObject",300,407,{"name":92,"@type":93},"Ophelia","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main aim of the study on Galangin and TNBC?","Question",{"text":112,"@type":113},"The study aims to assess the therapeutic efficacy of Galangin in treating triple-negative breast cancer by evaluating its effects on apoptotic regulation.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the research design combine computational and laboratory approaches?",{"text":117,"@type":113},"It uses in silico network pharmacology to identify gene targets and signaling pathways influenced by Galangin, then validates these predictions with multiple in vitro assays.",{"name":119,"@type":110,"acceptedAnswer":120},"Which gene and biological process are emphasized in the findings?",{"text":121,"@type":113},"The pro-apoptotic gene BAX is upregulated, alongside evidence of late-stage apoptosis and anti-proliferative effects in TNBC cells.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},340817,1790586454,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},7971461741311,"https://ap-avatar.wpscdn.com/avatar/74000253aff267980c6?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779345379180704826","Sinnarkar et al.  \nJournal of the Egyptian National Cancer Institute (2024) 36:41  \n[https://doi.org/10.1186/s43046-024-00246-y](https://doi.org/10.1186/s43046-024-00246-y)  \nJournal of the Egyptian National Cancer Institute  \n RESEARCH Open Access  \nGalangin promotes apoptosis by upregulating the pro-apoptotic gene BAXin triple-negative breast cancer  \nShruti Sinnarkar1, Poonam Suryawanshi2, Amol Dilip3, Jitendra Bhawalkar4 and Vaibhav Ladke2*  \nAbstract  \nBackground Triple-negative breast cancer (TNBC) is one of the most aggressive and formidable subtypes of breast cancer, devoid of targeted therapy and frequently leading to unfavorable prognoses and significant side effects. The demand for creative and effective treatment options has prompted the current study to investigate the potential of natural chemicals as therapeutic agents. This study intends to examine the efficacy of Galangin, a naturally occurring flavonoid, in treating triple-negative breast cancer.  \nMethods The research utilizes a dual methodology, combining in silico network pharmacology with in vitro experimental methods. The in silico research proved crucial in finding significant gene targets and cellular signaling pathways influenced by Galangin in triple-negative breast cancer. To corroborate these computational predictions, a variety of in vitro studies were conducted, including the MTT assay, wound scratch assay, apoptosis assay, reactive oxygen species assay, mitochondrial membrane potential assessment, and RT-PCR.  \nResults Fifteen prevalent genes were identified, demonstrating involvement in cellular proliferation, apoptosis regulation, cell migration, MAPK cascade regulation, and cell cycle regulation. The predominant genes implicated in the ten principal pathways were MAPK1, MAPK8, MAPK14, and IL6, which were observed to be linked to the MAPK signaling pathway, perhaps serving as the critical channel through which Galangin may facilitate the treatment of oral cancer. In vitro experiments demonstrated anti-proliferative effects, late-stage apoptosis, anti-migratory characteristics, antioxidant activity, and upregulation of the pro-apoptotic BAX gene.  \nConclusion This study’s results demonstrate that Galangin possesses considerable anti-proliferative effects onTNBC cells, underscoring its potential as a viable therapeutic drug. These findings facilitate the development of more effective and precisely focused therapy approaches forTNBC, providing optimism for enhanced treatment outcomes for patients suffering from this challenging disease.  \nKeywords Triple-negative breast cancer, Galangin, In silico analysis, Apoptosis, MAD MB-231  \n*Correspondence:  \nVaibhav Ladke  \n[drvaibhavladke@gmail.com](drvaibhavladke@gmail.com)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/licenses/by/4.0/](http://creativecommons.org/licenses/by/4.0/.)[.](http://creativecommons.org/licenses/by/4.0/.)  \nSinnarkar etal. Journal of the Egyptian National Cancer Institute (2024) 36:41 Page 2 of 18  \nIntroduction  \nCancer is a leading cause of mortality globally and is projected to rise concomitantly with popula","cbCaicWqQASdwbS4","https://ap.wps.com/l/cbCaicWqQASdwbS4","pdf",3229641,18,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusion\n# Introduction\n## Global cancer burden and risk factors\n## Breast cancer epidemiology\n## TNBC definition and clinical challenges","[{\"question\":\"What is the main aim of the study on Galangin and TNBC?\",\"answer\":\"The study aims to assess the therapeutic efficacy of Galangin in treating triple-negative breast cancer by evaluating its effects on apoptotic regulation.\"},{\"question\":\"How does the research design combine computational and laboratory approaches?\",\"answer\":\"It uses in silico network pharmacology to identify gene targets and signaling pathways influenced by Galangin, then validates these predictions with multiple in vitro assays.\"},{\"question\":\"Which gene and biological process are emphasized in the findings?\",\"answer\":\"The pro-apoptotic gene BAX is upregulated, alongside evidence of late-stage apoptosis and anti-proliferative effects in TNBC cells.\"}]","Galangin promotes apoptosis by upregulating the pro-apoptotic gene BAX in triple-negative breast cancer | PDF",1790038987,45]