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ESM1 levels are quantified in tumor and adjacent normal tissues, followed by shRNA-mediated knockdown in TPC-1 and SW579 cell lines. Cell proliferation, apoptosis, wound healing, and transwell assays assess growth, migration, and invasion. Results show higher ESM1 in PTC tissues, with knockdown significantly suppressing proliferation, migration, and invasion. ESM1 is proposed as a diagnostic and therapeutic target for PTC.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/functional-analysis-of-esm1-by-shrna-mediated-knockdown-of-its-expression-in-papillary-thyroid-cancer-cells/330443/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/functional-analysis-of-esm1-by-shrna-mediated-knockdown-of-its-expression-in-papillary-thyroid-cancer-cells/330443.png","ImageObject",300,407,{"name":92,"@type":93},"Ava Thompson","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-28","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What was the main objective of the study on ESM1 in papillary thyroid cancer?","Question",{"text":112,"@type":113},"To determine how ESM1 affects growth, migration, and invasion of papillary thyroid cancer cells and to clarify its potential role in PTC treatment.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How was ESM1 expression measured in patient tissues?",{"text":117,"@type":113},"ESM1 expression levels were detected in 53 PTC tumor samples and 59 matched adjacent normal tissue samples using immunohistochemical analysis.",{"name":119,"@type":110,"acceptedAnswer":120},"What effects did shRNA-mediated ESM1 knockdown have on PTC cells?",{"text":121,"@type":113},"Knockdown reduced ESM1 mRNA and protein levels and significantly inhibited proliferation, migration, and invasion in TPC-1 and SW579 cells in vitro.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},330443,1790048393,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":36},1649267921044,"https://us-avatar.wpscdn.com/avatar/1800007509477c92dfb?_k=1786009248482753345","PLOS ONE  \nOPEN ACCESS  \nCitation: Xie L, He L, Zhang W, Wang H (2024) Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells. PLoS ONE 19(4): e0298631 . [https://](https://)[ ](https://)[doi.org/10.1371/journal.pone.0298631](doi.org/10.1371/journal.pone.0298631)  \nEditor: Amirmohammad Khalaji, Tehran University of Medical Sciences, ISLAMIC REPUBLIC OF IRAN  \nReceived: October 17, 2023  \nAccepted: January 27, 2024  \nPublished: April 16, 2024  \nCopyright: © 2024 Xie et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  \nData Availability Statement: All relevant data are within the paper and its Supporting Information files.  \nFunding: The present study was funded by the Natural Science Foundation of China (grant nos. 81960320), Basic research project of Science and Technology Department of Yunnan Province (grant nos. 202101AT070148), Science and Technology Project of Sichuan Provincial Health Commission (23LCYJ023), and the Natural Science Foundation of Sichuan Province (grant nos. 2023NSFSC0635) . The funders had no role in study design, data  \nRESEARCH ARTICLE  \nFunctional analysis of ESM1 by shRNAmediated knockdown of its expression in papillary thyroid cancer cells  \nLijun Xie1, Limeng He2, Wei Zhang2‡*, Hao Wang2‡*  \n1 Department of Nuclear Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, P.  \nR. China, 2 Department of Nuclear Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, P. R. China  \n‡ HW and WZ are equal senior authorship to this work.  \n* [zhangwscd@uestc.edu.cn](zhangwscd@uestc.edu.cn) (WZ);  [18810566155@163.com](18810566155@163.com) (HW)  \nAbstract  \nObjective  \nEndothelial specific molecule-1 (ESM1) is implicated as an oncogene in multiple human cancers. However, the function of ESM1 in papillary thyroid cancer (PTC) is not well understood. The current study aimed to investigate the effect of ESM1 on the growth, migration, and invasion of PTC to provide a novel perspective for PTC treatment.  \nMethods  \nThe expression levels of ESM1 in PTC tissues form 53 tumor tissue samples and 59 matching adjacent normal tissue samples were detected by immunohistochemical analysis. Knockdown of ESM1 expression in TPC-1 and SW579 cell lines was established to investigate its role in PTC. Moreover, cell proliferation, apoptosis, wound healing, and transwell assays were conducted in vitro to assess cell proliferation, migration and invasion.  \nResults  \nThe findings revealed that ESM1 expression was significantly higher in PTC tissues than that found in paraneoplastic tissues (P\u003C0.0001) . Knockdown of ESM1 expression inhibited the proliferation, migration, and invasion of TPC-1 and SW579 cells in vitro. Compared with the control group, the mRNA and protein levels of ESM1 in PTC cells were significantly reduced following knockdown of its expression (P\u003C0.01) . In addition, ESM1-knockdown cells indicated decreased proliferation and decreased migratory and invasive activities (P\u003C0.01, P\u003C0.01, P\u003C0.001, respectively) .  \nConclusions  \nESM1 was identified as a major gene in the occurrence and progression of PTC, which could increase the proliferation, migration, and invasion of PTC cells. It may be a promising diagnostic and therapeutic target gene.  \ncollection and analysis, decision to publish, or preparation of the manuscript.  \nCompeting interests: The authors have declared that no competing interests exist.  \nIntroduction  \nThyroid cancer, with an estimated 586,202 new cases worldwide in 2020 alone, stands as a significant public health concern [ 1] . Its incidence, particularly in affluent regions, has experienced a marked increase, attributed to improved detection methods and environmental facto","cbCaienTmENpRngx","https://ap.wps.com/l/cbCaienTmENpRngx","pdf",3576897,16,"English","# Abstract\n## Objective\n## Methods\n## Results\n## Conclusions","[{\"question\":\"What was the main objective of the study on ESM1 in papillary thyroid cancer?\",\"answer\":\"To determine how ESM1 affects growth, migration, and invasion of papillary thyroid cancer cells and to clarify its potential role in PTC treatment.\"},{\"question\":\"How was ESM1 expression measured in patient tissues?\",\"answer\":\"ESM1 expression levels were detected in 53 PTC tumor samples and 59 matched adjacent normal tissue samples using immunohistochemical analysis.\"},{\"question\":\"What effects did shRNA-mediated ESM1 knockdown have on PTC cells?\",\"answer\":\"Knockdown reduced ESM1 mRNA and protein levels and significantly inhibited proliferation, migration, and invasion in TPC-1 and SW579 cells in vitro.\"}]","Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells | PDF",1789988946]