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Using MeRIP-seq/qPCR, dot blot, RNA/protein stability assays, and functional tumor models, the research links FTO-driven m6A demethylation in MTUS1/ATIP1 to transcript degradation and tumor-promoting behavior.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/fto-mediated-demethylation-of-mtus1atip1-promotes-tumor-progression-in-head-and-neck-squamous-cell-carcinoma/340533/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/fto-mediated-demethylation-of-mtus1atip1-promotes-tumor-progression-in-head-and-neck-squamous-cell-carcinoma/340533.png","ImageObject",300,407,{"name":42,"@type":43},"Evangeline","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-27","2026-09-22",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",8,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What problem does the study address regarding MTUS1/ATIP1 in HNSCC?","Question",{"text":63,"@type":64},"It examines why MTUS1/ATIP1 expression is reduced in HNSCC, even though MTUS1/ATIP1 has shown anti-cancer properties in earlier work.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How was the relationship between FTO and MTUS1/ATIP1 investigated?",{"text":68,"@type":64},"The study collected HNSCC and adjacent normal tissues and used m6A MeRIP-seq, qRT-PCR, and IHC, along with MeRIP-qPCR, m6A dot blot, and stability assays, to determine how FTO mediates demethylation of MTUS1/ATIP1.",{"name":70,"@type":61,"acceptedAnswer":71},"What did the results show about FTO’s mechanism and tumor effects?",{"text":72,"@type":64},"FTO was upregulated in HNSCC and negatively correlated with MTUS1/ATIP1. FTO mediated m6A demethylation in the 3’UTR of MTUS1/ATIP1, promoting degradation, and silencing MTUS1/ATIP1 reversed the tumor-promoting effects of FTO in vitro and in vivo.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},340533,1790169441,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":108,"slug":109},7,40,"healthcare",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":111,"show_sort_weight":112,"slug":113},"Research & Report",30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":107,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","Tang et al. BMC Cancer (2024) 24:1489 BMC Cancer  \n[https://doi.org/10.1186/s12885-024-13253-y](https://doi.org/10.1186/s12885-024-13253-y)  \nRESEARCH Open Access  \nFTO-mediated demethylation of MTUS1/ ATIP1 promotes tumor progression in head and neck squamous cell carcinoma  \nDongxiao Tang 1,2†, Congyuan Cao 1†, Wuguo Li3 and Anxun Wang 1*  \nAbstract  \nBackground Head and neck squamous cell carcinoma (HNSCC) has been recognized as the seventh most prevalent malignant tumor globally. It is a malignant neoplasm that arises from the mucosal epithelium of head and neck region. In our previous research, we have demonstrated that MTUS1/ATIP1 exhibits anti-cancer properties in HNSCC. Nevertheless, the underlying mechanism responsible for the reduction of MTUS1/ATIP1 expression has not been investigated.  \nMethods HNSCC and adjacent normal tissues were collected and examined using m6A MeRIP-seq, qRT-PCR, and IHC to investigate the relationship between MTUS1/ATIP1 and FTO. MeRIP-qPCR, m6A dot blot, RNA and protein stability assays, and RNC-qRT-PCR were employed to elucidate the mechanism by which FTO mediates demethylation of MTUS1/ATIP1 in HNSCC. Functional assays, subcutaneous tumorigenesis, and in situ tongue cancer models were conducted to assess the impact of the FTO-MTUS1/ATIP1 pathway on proliferative capacity of HNSCC tumors.  \nResults FTO was observed to be markedly upregulated and showed a negative correlation with MTUS1/ATIP1 expression in HNSCC. FTO was responsible for mediating m6A demethylation in the 3’UTR of MTUS1/ATIP1, leading to its degradation. Additionally, silencing MTUS1/ATIP1 successfully reversed the tumor-promoting effects on HNSCC triggered by FTO in in vitro and in vivo.  \nConclusions Our research elucidated the functional importance of FTO-mediated m6A demethylation of MTUS1/ ATIP1, suggesting that targeting the FTO-MTUS1/ATIP1 axis could be a prospective novel approach for treating HNSCC.  \nKeywords MTUS1/ATIP1, Head and neck squamous cell carcinoma, FTO, N6-methyladenosine, Demethylation  \n†Dongxiao Tang and Congyuan Cao contributed equally to this work.  \n*Correspondence:  \nAnxun Wang  \n[wanganx@mail.sysu.edu.cn](wanganx@mail.sysu.edu.cn)  \n1Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, SunYat-Sen University, Guangzhou, Guangdong 510080, China 2Department of Stomatology, The Third Affiliated Hospital, SunYat-Sen University, Guangzhou, Guangdong 510630, China  \n3Animal Experiment Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong 510080, China  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creati](http://creati)[vecommons.org/l](vecommons.org/l)icenses/by-nc-nd/4.0/.  \nTang et al. BMC Cancer (2024) 24:1489  \nBackground  \nHead and neck squamous cell carcinoma (HNSCC) has been recognized as the seventh most prevalent malignant tumor globally. It is a malignant neoplasm that arises from the mucosal epithelium of head and neck region [1, 2]. Despite advancements in current strategies for treating advanced H","cbCaibYnJu4v9oT1","https://ap.wps.com/l/cbCaibYnJu4v9oT1","pdf",5839514,13,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Background\n## Clinical context and significance of MTUS1/ATIP1\n## Role of m6A methylation and FTO in malignancy","[{\"question\":\"What problem does the study address regarding MTUS1/ATIP1 in HNSCC?\",\"answer\":\"It examines why MTUS1/ATIP1 expression is reduced in HNSCC, even though MTUS1/ATIP1 has shown anti-cancer properties in earlier work.\"},{\"question\":\"How was the relationship between FTO and MTUS1/ATIP1 investigated?\",\"answer\":\"The study collected HNSCC and adjacent normal tissues and used m6A MeRIP-seq, qRT-PCR, and IHC, along with MeRIP-qPCR, m6A dot blot, and stability assays, to determine how FTO mediates demethylation of MTUS1/ATIP1.\"},{\"question\":\"What did the results show about FTO’s mechanism and tumor effects?\",\"answer\":\"FTO was upregulated in HNSCC and negatively correlated with MTUS1/ATIP1. FTO mediated m6A demethylation in the 3’UTR of MTUS1/ATIP1, promoting degradation, and silencing MTUS1/ATIP1 reversed the tumor-promoting effects of FTO in vitro and in vivo.\"}]","FTO-mediated demethylation of MTUS1/ATIP1 promotes tumor progression in head and neck squamous cell carcinoma | PDF",1790037175,33]