[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-330151-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-330151-en":130},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","fractionation-and-phytochemical-composition-of-an-ethanolic-extract-of-ziziphus-nummularia-leaves-antioxidant-and-anticancerous-properties-in-human-triple-negative-breast-cancer-cells","Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves - antioxidant and anticancerous properties in human triple negative breast cancer cells","","Natural products have long supported traditional medicine and have become important sources for modern drug discovery, with renewed interest in bioactives that complement synthetic therapies, especially as cancer incidence and mortality rise globally. Ziziphus nummularia leaves were extracted with ethanol and fractionated; phytochemical composition was characterized qualitatively and analytically using spectrophotometric methods and HPLC-PDA-MS/MS. Fractions, particularly Fraction 6, reduced viability of MDA-MB-231 triple negative breast cancer cells more strongly than crude extract. ZNE and F6 showed antioxidant capacity in DPPH, while increasing ROS and triggering cell cycle arrest, apoptosis and autophagy-mediated death and inhibiting migration, invasion, and adhesion, indicating potential chemopreventive and anticancer lead opportunities.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/fractionation-and-phytochemical-composition-of-an-ethanolic-extract-of-ziziphus-nummularia-leaves-antioxidant-and-anticancerous-properties-in-human-triple-negative-breast-cancer-cells/330151/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/fractionation-and-phytochemical-composition-of-an-ethanolic-extract-of-ziziphus-nummularia-leaves-antioxidant-and-anticancerous-properties-in-human-triple-negative-breast-cancer-cells/330151.png","ImageObject",300,407,{"name":42,"@type":43},"Patrick","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-30","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",6,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"How was the ethanolic extract of Ziziphus nummularia leaves fractionated and analyzed?","Question",{"text":63,"@type":64},"The phytochemical composition of the ethanolic extract and its chromatographically isolated fractions was identified using spectrophotometric assays and analytically by HPLC-PDA-MS/MS.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What effect did ZNE and Fraction 6 have on triple-negative breast cancer cells?",{"text":68,"@type":64},"ZNE and Fraction 6 reduced the viability of the MDA-MB-231 triple negative breast cancer cell line, with Fraction 6 decreasing viability more than crude extract or other fractions.",{"name":70,"@type":61,"acceptedAnswer":71},"How do ZNE and Fraction 6 relate antioxidant activity to ROS production and cell death?",{"text":72,"@type":64},"Although both showed potent antioxidant capacity in the DPPH assay, they promoted reactive oxygen species (ROS) production in MDA-MB-231 cells; this ROS-related effect and viability reduction were blunted by the antioxidant N-acetyl cysteine (NAC). ZNE and Fraction 6 also induced cell cycle arrest and triggered apoptosis and autophagy-mediated cell death.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},330151,1790164358,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,105,110,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":55,"doc_module":4,"doc_module_name":25,"category_name":102,"show_sort_weight":103,"slug":104},"Technology",50,"technology",{"id":106,"doc_module":4,"doc_module_name":25,"category_name":107,"show_sort_weight":108,"slug":109},7,"Healthcare",40,"healthcare",{"id":111,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":112,"slug":113},8,30,"research-report",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":117,"slug":118},9,"Religion & Spirituality",20,"religion-spirituality",{"id":117,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":117,"slug":121},"World Cup","world-cup",{"id":123,"doc_module":4,"doc_module_name":25,"category_name":124,"show_sort_weight":123,"slug":125},10,"Lifestyle","lifestyle",{"id":127,"doc_module":4,"doc_module_name":25,"category_name":128,"show_sort_weight":97,"slug":129},19,"General","general",{"code":4,"msg":82,"data":131},{"doc_id":79,"user_id":132,"nickname":42,"user_avatar":133,"doc_module":4,"category_id":111,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":139,"language":140,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":12,"update_tm":144,"read_time":145,"preview_page_count":4},549758146520,"https://ap-avatar.wpscdn.com/avatar/80002397d8c0411e94?_k=1775819394049821470","TYPE Original Research PUBLISHED 09 February 2024 DOI 10.3389/fphar.2024.1331843  \nOPEN ACCESS  \nEDITED BY  \nNunziatina De Tommasi, University of Salerno, Italy  \nREVIEWED BY  \nMaria Laura Bellone, University of Salerno, Italy Teodora Alexa-Stratulat,  \nGrigore T. Popa University of Medicine and Pharmacy, Romania  \n*CORRESPONDENCE  \nAbdullah A. Shaito,  \n [abdshaito@qu.edu.qa](abdshaito@qu.edu.qa),  \n [abdshaito@gmail.com](abdshaito@gmail.com)[ ](abdshaito@gmail.com)Joelle Edward Mesmar,  \n [jm104@aub.edu.lb](jm104@aub.edu.lb)[ ](jm104@aub.edu.lb)Elias Baydoun,  \n [eliasbay@aub.edu.lb](eliasbay@aub.edu.lb)  \nRECEIVED 01 November 2023  \nACCEPTED 09 January 2024  \nPUBLISHED 09 February 2024  \nCITATION  \nAbdallah R, Shaito AA, Badran A, Baydoun S, Sobeh M, Ouchari W, Sahri N, EidAH, Mesmar JEand Baydoun E (2024), Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves:  \nantioxidant and anticancerous properties inhuman triple negative breast cancer cells. Front. Pharmacol. 15:1331843 .  \ndoi: 10.3389/fphar.2024.1331843  \nCOPYRIGHT  \n© 2024 Abdallah, Shaito, Badran, Baydoun, Sobeh, Ouchari, Sahri, Eid, Mesmar and Baydoun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.  \nFractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties inhuman triple negative breast cancer cells  \nRola Abdallah 1, Abdullah A. Shaito 2*, Adnan Badran 3,  \nSerine Baydoun 4, Mansour Sobeh 5, Wafae Ouchari 5, Nihad Sahri 5, Ali H. Eid 6, Joelle Edward Mesmar 1* and Elias Baydoun 1*  \n1Department of Biology, American University of Beirut, Beirut, Lebanon, 2Biomedical Research Center, Department of Biomedical Sciences at College of Health Sciences, and College of Medicine, Qatar University, Doha, Qatar, 3Department of Nutrition, University of Petra, Amman, Jordan, 4Breast Imaging Section, Imaging Institute, Cleveland Clinic Foundation, Cleveland, OH, United States, 5Agrobiosciences Program, College for Agriculture and Environmental Science, Mohammed VI Polytechnic University, Ben Guerir, Morocco, 6Department of Basic Medical Sciences, College of Medicine, QU Health, Qatar University, Doha, Qatar  \nNatural products have long been utilized in traditional medicine as remedies to improve health and treat illnesses, and have had a key role in modern drug discovery. Recently, there has been a revived interest in the search for bioactives from natural sources as alternative or complementary modalities to synthetic medicines; especially for cancer treatment, which incidence and mortality rates are on the rise worldwide. Ziziphus nummularia has been widely used in traditional medicine for the treatment of various diseases. Its traditional uses and numerous ethnopharmacological properties may be attributed to its richness in bioactive metabolites. However, its phytochemical composition or chemopreventive effects against the aggressive triple-negative breast cancer (TNBC) are still poorly explored. Here, phytochemical composition of an ethanolic extract of Z. nummularia leaves (ZNE) and its chromatographically isolated fractions was identified both qualitatively by spectrophotometric assaysand analytically by HPLC-PDA-MS/MS. The anti-proliferative effects of ZNE were tested in several cancer cell lines, but we focused on its anti-TNBC effects since they were not explored yet. The anti-cancerous potential of ZNE and its fractions was tested in vitro in MDA-MB-231, a TNBC cell line. Results showed that ZNE and its Fraction 6 (F6) reduced the viability of MDA-","cbCaipXCCyTnGTw2","https://ap.wps.com/l/cbCaipXCCyTnGTw2","pdf",3786909,22,"English","# Introduction\n## Anticancer relevance of plant-derived metabolites\n## Rationale for targeting triple-negative breast cancer","[{\"question\":\"How was the ethanolic extract of Ziziphus nummularia leaves fractionated and analyzed?\",\"answer\":\"The phytochemical composition of the ethanolic extract and its chromatographically isolated fractions was identified using spectrophotometric assays and analytically by HPLC-PDA-MS/MS.\"},{\"question\":\"What effect did ZNE and Fraction 6 have on triple-negative breast cancer cells?\",\"answer\":\"ZNE and Fraction 6 reduced the viability of the MDA-MB-231 triple negative breast cancer cell line, with Fraction 6 decreasing viability more than crude extract or other fractions.\"},{\"question\":\"How do ZNE and Fraction 6 relate antioxidant activity to ROS production and cell death?\",\"answer\":\"Although both showed potent antioxidant capacity in the DPPH assay, they promoted reactive oxygen species (ROS) production in MDA-MB-231 cells; this ROS-related effect and viability reduction were blunted by the antioxidant N-acetyl cysteine (NAC). ZNE and Fraction 6 also induced cell cycle arrest and triggered apoptosis and autophagy-mediated cell death.\"}]","Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves - antioxidant and anticancerous properties in human triple negative breast cancer cells | PDF",1789987431,55]