[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-501112-105":59,"doc-detail-501112-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","flow-cytometry-basics-guide","Flow Cytometry Basics Guide","","Flow Cytometry Basics Guide provides a structured introduction to flow cytometers and how fluorescence signals are measured, processed, and analyzed. It covers core instrument principles, fluorescence and dye selection, compensation and control strategies, and practical experimental optimization including sample preparation, live/dead exclusion, autofluorescence, doublet discrimination, and fixation. It also guides multicolor panel building and introduces common applications such as immunophenotyping, apoptosis, proliferation and cell cycle, signaling, and emerging innovations.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/flow-cytometry-basics-guide/501112/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/flow-cytometry-basics-guide/501112.png","ImageObject",300,407,{"name":92,"@type":93},"pixelkiddo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-23","2026-10-03",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What are the key principles behind a flow cytometer’s fluidics system and detection?","Question",{"text":112,"@type":113},"The guide explains the instrument’s optics and detection approach, how signals are converted into measurable events, and how pulse processing supports downstream analysis. It also introduces electrostatic cell sorting as part of the system’s operation.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Why are fluorescence compensation and compensation controls necessary?",{"text":117,"@type":113},"Fluorescence compensation is needed because signals from overlapping emission spectra can distort measurements. Compensation controls are used to quantify and correct these overlaps so multicolor results remain reliable.",{"name":119,"@type":110,"acceptedAnswer":120},"How do you optimize a flow cytometry experiment before collecting data?",{"text":121,"@type":113},"The guide covers sample preparation and key quality steps: live/dead exclusion, minimizing autofluorescence, doublet discrimination, and collecting enough cells for statistical relevance. For intracellular targets, it also covers permeabilization and fixation.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},501112,1791358169,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":21,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},962090883892,"https://ap-avatar.wpscdn.com/avatar/e00115db34f3e0f11b?x-image-process=image/resize,m_fixed,w_180,h_180&k=1790152879550218658","Flow Cytometry  \nFlow Cytometry Basics Guide  \nTable of Contents  \nChapter 1 Principles of the Flow Cytometer Fluid ics System  .......................................................................... 3  \nOptics and Detection  ...................................................................... 4  \nSignal and Pulse Processing  ................................................................ 6  \nElectrostatic Cell Sorting  ................................................................... 9  \nChapter 2 Principles of Fluorescence Fluoro phores and Light  ................................................................... 11  \nFluorescence  ........................................................................... 12  \nWhy Use a Fluorescent Marker?  ............................................................ 13  \nWhich Fluoro phores are Useful for Flow Cytometry?  ............................................. 13  \nSingle and Tandem Dyes  ................................................................ 14  \nFluorescent Proteins  ................................................................... 14  \nFluorescence Compensation  ............................................................... 16  \nCompensation Controls  ................................................................. 18  \nChapter 3 Data Analysis  \nGates and Regions  ...................................................................... 19  \nSingle-Parameter or Univariate Histograms  .................................................... 21  \nTwo-Parameter or Bivariate Histograms  ...................................................... 22  \nBackgating to Confirm Gating Strategies  ...................................................... 24  \nChapter 4 Controls in Flow cytometry Unstained Controls  ...................................................................... 25  \nIsotype Controls  ........................................................................ 26  \nSingle Staining and Compensation Controls  ................................................... 27  \nFc Blocking Controls  ..................................................................... 28  \nFluorescence Minus One Controls  ........................................................... 29  \nIntracellular Staining Controls  .............................................................. 30  \nBiological Controls  ...................................................................... 30  \nChapter 5 Optimizing your Experiments Sample Preparation  ...................................................................... 31  \nLive/Dead Exclusion  ..................................................................... 32  \nAutofluorescence  ........................................................................ 34  \nDo ublet Discrimination  .................................................................... 34  \nCollect a Statistically Relevant Number of Cells  ................................................. 35  \nPer meabi lization and Fixation for Intracellular Antigens  ........................................... 36  \nFlow Cytometry Basics Guide | 1  \nChapter 6 Multicolor Panel Building Resolution of Signal  ...................................................................... 37  \nInstrument Configuration  .................................................................. 38  \nFluoro phore Separation  ................................................................... 38  \nAntigen Density  ......................................................................... 38  \nFluoro phore Properties  ................................................................... 38  \nMarker Expression Patterns  ............................................................... 39  \nDump Channels  ......................................................................... 39  \nAntibody Titration  ....................................................................... 39  \nPanel Building Tools  ......................................","cbCaivyeeFAVcA2j","https://ap.wps.com/l/cbCaivyeeFAVcA2j","pdf",1181676,"English","# Chapter 1 Principles of the Flow Cytometer Fluidics System\n## Optics and Detection\n## Signal and Pulse Processing\n## Electrostatic Cell Sorting\n# Chapter 2 Principles of Fluorescence\n## Fluorescence\n## Why Use a Fluorescent Marker?\n## Which Fluorophores are Useful for Flow Cytometry?\n## Single and Tandem Dyes\n## Fluorescent Proteins\n## Fluorescence Compensation\n## Compensation Controls\n# Chapter 3 Data Analysis\n## Gates and Regions\n## Single-Parameter or Univariate Histograms\n## Two-Parameter or Bivariate Histograms\n## Backgating to Confirm Gating Strategies\n# Chapter 4 Controls in Flow Cytometry\n## Unstained Controls\n## Isotype Controls\n## Single Staining and Compensation Controls\n## Fc Blocking Controls\n## Fluorescence Minus One Controls\n## Intracellular Staining Controls\n## Biological Controls\n# Chapter 5 Optimizing your Experiments\n## Sample Preparation\n## Live/Dead Exclusion\n## Autofluorescence\n## Doublet Discrimination\n## Collect a Statistically Relevant Number of Cells\n## Permeabilization and Fixation for Intracellular Antigens\n# Chapter 6 Multicolor Panel Building\n## Resolution of Signal\n## Instrument Configuration\n## Fluorophore Separation\n## Antigen Density\n## Fluorophore Properties\n## Marker Expression Patterns\n## Dump Channels\n## Antibody Titration\n## Panel Building Tools\n# Chapter 7 Common Applications and New Technology\n## Immunophenotyping\n## Apoptosis\n## Proliferation and Cell Cycle\n## Signaling and Phosphoflow\n## Small Particle Detection\n## Gene Expression and Transfection\n## Absolute Quantification\n## Particle Internalization\n## Fluorescence in situ Hybridization and RNA Detection\n## Innovations in Flow Cytometry\n## Recommended Reading\n# Chapter 8 Common Protocols\n## Sample Preparation\n## Preparation of Cells for Flow Cytometry\n## Preparation of Tissue Culture Cells Stored in Liquid Nitrogen\n## Preparation of Tissue Culture Cells in Suspension\n## Preparation of Adherent Tissue Culture Cell Lines\n## Preparation of Human Peripheral Blood Mononuclear Cells\n## Preparation of Peritoneal Macrophages, Bone Marrow, Thymus, and Spleen Cells\n## Direct Immunofluorescence Staining of Surface Epitopes of Cells and Blood","[{\"question\":\"What are the key principles behind a flow cytometer’s fluidics system and detection?\",\"answer\":\"The guide explains the instrument’s optics and detection approach, how signals are converted into measurable events, and how pulse processing supports downstream analysis. It also introduces electrostatic cell sorting as part of the system’s operation.\"},{\"question\":\"Why are fluorescence compensation and compensation controls necessary?\",\"answer\":\"Fluorescence compensation is needed because signals from overlapping emission spectra can distort measurements. Compensation controls are used to quantify and correct these overlaps so multicolor results remain reliable.\"},{\"question\":\"How do you optimize a flow cytometry experiment before collecting data?\",\"answer\":\"The guide covers sample preparation and key quality steps: live/dead exclusion, minimizing autofluorescence, doublet discrimination, and collecting enough cells for statistical relevance. For intracellular targets, it also covers permeabilization and fixation.\"}]","Flow Cytometry Basics Guide | PDF",1791017346,176]