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This study presents the GlyExo-Capture method to isolate fucosylated extracellular vesicles (Fu-EVs) from serum, followed by next-generation sequencing-based profiling of microRNAs. Five miRNAs (hsa-let-7a, hsa-miR-21, hsa-miR-125a, hsa-miR-200a, hsa-miR-150) form a diagnostic panel with high sensitivity and specificity, improved performance versus AFP and DCP, strong early-stage recall, and effective discrimination from other malignancies.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/five-mirnas-identified-in-fucosylated-extracellular-vesicles-as-non-invasive-diagnostic-signatures-for-hepatocellular-carcinoma/329284/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/five-mirnas-identified-in-fucosylated-extracellular-vesicles-as-non-invasive-diagnostic-signatures-for-hepatocellular-carcinoma/329284.png","ImageObject",300,407,{"name":92,"@type":93},"Skyler","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-06","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":39},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the GlyExo-Capture method used for in this study?","Question",{"text":112,"@type":113},"It is used to isolate fucosylated extracellular vesicles (Fu-EVs) from serum so their microRNA profiles can be analyzed for HCC diagnosis.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which microRNAs are identified as biomarkers for hepatocellular carcinoma?",{"text":117,"@type":113},"The study identifies a five-miRNA set: hsa-let-7a, hsa-miR-21, hsa-miR-125a, hsa-miR-200a, and hsa-miR-150.",{"name":119,"@type":110,"acceptedAnswer":120},"How does the five-miRNA panel compare with standard markers like AFP and DCP?",{"text":121,"@type":113},"The panel shows exceptional diagnostic performance, with reported sensitivity and specificity that significantly surpass AFP and des-gamma-carboxy prothrombin (DCP).","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},329284,1790816271,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":39,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145,"preview_page_count":4},2336464648746,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Article  \nFive miRNAs identified in fucosylated extracellular vesicles as non-invasive diagnostic signatures for hepatocellular carcinoma  \nGraphical abstract  \nHighlights  \nAuthors  \nBoan Li, Kun Hao, Mengyang Li, ..., Zhiming Zhao, Ronghua Jin, Ruifu Yang  \nCorrespondence  \n[gaoqi@youngen.net.cn](gaoqi@youngen.net.cn) (Q.G.),  \n[zhimingzhao616@126.com](zhimingzhao616@126.com) (Z.Z.),  \n[ronghuajin@ccmu.edu.cn](ronghuajin@ccmu.edu.cn) (R.J.),  \n[ruifuyang@gmail.com](ruifuyang@gmail.com) (R.Y.)  \nIn brief  \nLi and colleagues present the GlyExoCapture method for isolating fucosylated extracellular vesicles (Fu-EVs) and identify five miRNAs for early HCC diagnosis. The method effectively detects early-stage HCC and differentiates it from other cancers, offering a highthroughput, rapid, and non-invasive screening approach.  \nd Introduction of the GlyExo-Capture approach for isolating extracellular vesicles  \nd Identification and validation of five miRNAs with strong diagnostic performance for HCC  \nd Effective detection of early-stage HCC and differentiation from other malignancies  \nd Development of a high-throughput, rapid, and non-invasive method for early HCC diagnosis  \nLi et al., 2024, Cell Reports Medicine 5, 101716  \nSeptember 17, 2024 ª 2024 The Authors. Published by Elsevier Inc.  \n[https://doi.org/10.1016/j.xcrm.2024.101716](https://doi.org/10.1016/j.xcrm.2024.101716)  \nll  \nll  \nOPEN ACCESS  \nArticle  \nFive miRNAs identified in fucosylated extracellular vesicles as non-invasive diagnostic signatures for hepatocellular carcinoma  \nBoan Li,1,8 Kun Hao,2,7,8 Mengyang Li,3,8 Ailan Wang,2 Huixue Tang,2 Lida Xu,2 Cuidie Ma,2 Wenqian Du,2 Lijuan Sun,4 Xufeng Hou,4 Tianye Jia,1 Aixia Liu,1 Qi Gao,2,4,9,* Zhiming Zhao,3,* Ronghua Jin,5,* and Ruifu Yang6,7,*  \n1Department of Laboratory Medicine, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China  \n2Beijing Hotgen Biotech Co. , Ltd. , Beijing, China  \n3The Faculty of Hepatopancreatobiliary Surgery, The First Medical Center of Chinese PLA General Hospital, Beijing, China  \n4Beijing Youngen Technology Co. , Ltd. , Beijing, China  \n5Capital Medical University Affiliated Beijing Ditan Hospital, Beijing, China  \n6Beijing Key Laboratory of POCT for Bioemergency and Clinic (No. BZ0329), Beijing, China  \n7State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China  \n8These authors contributed equally  \n9Lead contact  \n*Correspondence: [gaoqi@youngen.net.cn](gaoqi@youngen.net.cn) (Q.G.), [zhimingzhao616@126.com](zhimingzhao616@126.com) (Z.Z.), [ronghuajin@ccmu.edu.cn](ronghuajin@ccmu.edu.cn) (R.J.), [ruifuyang@gmail.com](ruifuyang@gmail.com)  \n(R.Y.)  \n[https://doi.org/10.1016/j.xcrm.2024.101716](https://doi.org/10.1016/j.xcrm.2024.101716)  \nSUMMARY  \nHepatocellular carcinoma (HCC) is a prevalent and aggressive cancer that presents significant challenges for early detection. This study introduces the GlyExo-Capture method for isolating fucosylated extracellular vesicles (Fu-EVs) from serum. We analyze microRNA (miRNA) profiles from Fu-EVs in 88 HCC patients and 179 non-HCC controls using next-generation sequencing (NGS) and identify five miRNAs (hsa-let-7a, hsa-miR- 21, hsa-miR-125a, hsa-miR-200a, and hsa-miR-150) as biomarkers for HCC diagnosis. The five-miRNA panel demonstrates exceptional HCC diagnostic performance, with a sensitivity of 0.90 and specificity of 0.92 in a combined cohort of 194 HCC and 412 non-HCC controls, significantly surpassing the performance of alphafetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP) . Notably, the miRNA model achieves recall rates of 85.7% and 90.8% for stage 0 and stage A early-stage HCC, respectively, identifies 88.1% of AFPnegative HCC cases, and effectively differentiates HCC from other cancers. This study provides a highthroughput, rapid, and non-invasive approach for early HCC detection.  \nINTRODUCTION  \nLiver cancer is a leading cause of cancer-related death globally,","cbCaibPF3vZhLN89","https://ap.wps.com/l/cbCaibPF3vZhLN89","pdf",6186426,18,"English","# Summary\n# Introduction","[{\"question\":\"What is the GlyExo-Capture method used for in this study?\",\"answer\":\"It is used to isolate fucosylated extracellular vesicles (Fu-EVs) from serum so their microRNA profiles can be analyzed for HCC diagnosis.\"},{\"question\":\"Which microRNAs are identified as biomarkers for hepatocellular carcinoma?\",\"answer\":\"The study identifies a five-miRNA set: hsa-let-7a, hsa-miR-21, hsa-miR-125a, hsa-miR-200a, and hsa-miR-150.\"},{\"question\":\"How does the five-miRNA panel compare with standard markers like AFP and DCP?\",\"answer\":\"The panel shows exceptional diagnostic performance, with reported sensitivity and specificity that significantly surpass AFP and des-gamma-carboxy prothrombin (DCP).\"}]","Five miRNAs identified in fucosylated extracellular vesicles as non-invasive diagnostic signatures for hepatocellular carcinoma | PDF",1789983437,45]