[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-398413-105":59,"doc-detail-398413-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","finetest-human-tsi-elisa-kit-instructions-for-use","FineTest Human TSI ELISA Kit - Instructions for Use","","Human TSI (Thyroid Stimulating Immunoglobulin) ELISA kit for in vitro quantitative measurement of TSI in serum, plasma, cell culture supernatant, and other biological samples. The assay is a sandwich ELISA format using a pre-coated 96-well plate, biotin-conjugated antigen, HRP-streptavidin, and TMB substrate with stop solution; results are read at OD450 and calculated via standard curve. Includes kit catalog and revision details, storage guidance, kit components, required instruments, and sample collection procedures.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/finetest-human-tsi-elisa-kit-instructions-for-use/398413/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/finetest-human-tsi-elisa-kit-instructions-for-use/398413.png","ImageObject",300,407,{"name":92,"@type":93},"River Wang","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-27",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",11,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the intended use of this Human TSI ELISA kit?","Question",{"text":113,"@type":114},"It is for in vitro quantitative determination of TSI concentrations in serum, plasma, cell culture supernatant, and other biological samples.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How does the assay generate the OD450 signal?",{"text":118,"@type":114},"HRP catalyzes TMB to produce a blue product that turns yellow after adding acidic stop solution, and the OD at 450 nm is measured for standard-curve calculation.",{"name":120,"@type":111,"acceptedAnswer":121},"How should opened kit components be stored?",{"text":122,"@type":114},"Sealed kits are stored at 2-8°C, and opened components should follow the specified storage conditions listed per item in the provided table (including desiccant handling and temperature guidance).","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},398413,1791035157,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":140,"language":141,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":67,"update_tm":145,"read_time":36,"preview_page_count":4},1099514067438,"https://ap-avatar.wpscdn.com/avatar/100002539ee87300030?x-image-process=image/resize,m_fixed,w_180,h_180&k=1780474512215547542","(For Research Use Only. Not For Use In Diagnostic Procedures!)  \nFineTest®  \nHuman TSI (Thyroid Stimulating Immunoglobulin) ELISA Kit  \nCatalogue No. : EH5041  \nRevision: V4 .0  \nSize: 48T/96T  \nPlease do not mix and use reagents from different kits or different batches. Otherwise, it might not work properly.  \nPlease read the manual carefully before use. Feel free to contact us if you have any questions.  \nEmail [fine@fn-test.com](fine@fn-test.com)  \nWebsite [https://www.fn-test.com/](https://www.fn-test.com/)  \nPlease provide the batch number (see kit label) for more rapid response and services.  \nIt's strongly recommended to use this kit within the expiry date printed on the kit label.  \n[www.fn-test.com](www.fn-test.com) 1 / 16  \nTechnical support related documents  \n\n| Title of\u003Cbr>Document | Sample preparation guide | Experimental operation procedure | TMB color rendering control | Standard curve and concentration calculation software\u003Cbr>CurveExpert1 .4(Including tutorial) |\n| --- | --- | --- | --- | --- |\n| Website | [https://www.fn](https://www.fn)[test.com/content/uploa](test.com/content/uploa)[ds/2022/06/ELISA-](ds/2022/06/ELISA-)Sample-PreparationProtocol-2022 .6.6. pdf | [https://www.fn](https://www.fn)[test.com/videos/elisa](test.com/videos/elisa)test/ | [https://www.fn](https://www.fn)[test.com/videos/targete](test.com/videos/targete)d-control-of-tmbcoloring/ | [https://www.fn](https://www.fn)[test.com/content/uploads/](test.com/content/uploads/)[ ](test.com/content/uploads/)[2019/08/CurveExpert-](2019/08/CurveExpert-)\u003Cbr>[1.4.zip](1.4.zip) |\n| Quick Mark | | | | |\n\nProduct Features  \n\n| Application | In vitro quantitative determination of TSI concentrations in serum, plasma, cell culture supernatant and other biological samples. |  |  |\n| --- | --- | --- | --- |\n| Reactivity | Human | Detection Method | Sandwich |\n| Range | 3. 125-200ng/ ml | Sensitivity | 1.875ng/ ml |\n| Detection Duration | 4 hours(excluding balancing and sample preparation) |  |  |\n| Samples needed for single well(Max) | Serum: 50 ul, Plasma: 50 ul, Cell Culture Supernatant: 100ul, cell or tissue lysate:\u003Cbr>100ul, Other liquid samples: 50ul |  |  |\n| Specificity | Specifically recognize TSI, no obvious cross reaction with other analogues |  |  |\n| Storage | 2-8° C (for sealed box), please do not freeze! See kit label for expiry date |  |  |\n\n[www.fn-test.com](www.fn-test.com) 2 / 16  \nPrinciple of the Assay  \nThis kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto the 96-well plate. The biotin conjugated antigen was used as the detection antigen . The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antigen was added to bind with TSI conjugated on coated antigen. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O. D. absorbance at 450nm in a microplate reader. The concentration of target antigen in the sample is positively correlated with OD450 and can be calculated by plotting the standard curve.  \nKit Components and Storage  \nThe sealed kit can be stored at 2-8 ℃ . The storage condition for opened kit is specified in the table below:  \n\n| No. | Item | Size(48T) | Size(96T) | Storage Condition for Opened Kit |\n| --- | --- | --- | --- | --- |\n| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8° C; Stored for 12 month at-20° C |\n| E002 | Lyophilized Standard | 1vial | 2vial | Put the rest standards into adesiccant bag. Stored for 1 month at 2-8° C; Stored for 12 month at-20° C |\n| E003 | Biotin-labeled Antigen (Concentrated, 100X) | 60ul | 120ul | 2-8°C (Avoid Direct Light) |\n","cbCairqpBRzAw6Jl","https://ap.wps.com/l/cbCairqpBRzAw6Jl","pdf",747013,16,"English","# Product Features\n## Principle of the Assay\n## Kit Components and Storage\n## Required Instruments and Reagents\n## Sample Collection and Storage","[{\"question\":\"What is the intended use of this Human TSI ELISA kit?\",\"answer\":\"It is for in vitro quantitative determination of TSI concentrations in serum, plasma, cell culture supernatant, and other biological samples.\"},{\"question\":\"How does the assay generate the OD450 signal?\",\"answer\":\"HRP catalyzes TMB to produce a blue product that turns yellow after adding acidic stop solution, and the OD at 450 nm is measured for standard-curve calculation.\"},{\"question\":\"How should opened kit components be stored?\",\"answer\":\"Sealed kits are stored at 2-8°C, and opened components should follow the specified storage conditions listed per item in the provided table (including desiccant handling and temperature guidance).\"}]","FineTest Human TSI ELISA Kit - Instructions for Use | PDF",1790479515]