[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-330897-105":59,"doc-detail-330897-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","findings-and-challenges-in-replacing-traditional-uterine-cervical-cancer-diagnosis-with-molecular-tools-in-private-gynecological-practice-in-mexico","Findings and Challenges in Replacing Traditional Uterine Cervical Cancer Diagnosis with Molecular Tools in Private Gynecological Practice in Mexico","","Encouraging practicing gynecologists to adopt molecular diagnostics such as PCR and cancer biomarkers as alternatives to traditional Papanicolaou and colposcopy approaches. Liquid-based cytology aliquots were used for HR HPV testing, PAP testing, and p16/Ki67 dual-stain cytology. Across 4499 laboratory samples, HR HPV-PCR was negative in 25.1% of low-grade and 47.9% of high-grade cases after PAP testing. Among PCR-positive HR HPV findings, 68.3% showed no evidence of cancerous cells, emphasizing dual staining after PCR to avoid unnecessary colposcopies and highlighting social reluctance toward abandoning Pap smears in Mexico.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/findings-and-challenges-in-replacing-traditional-uterine-cervical-cancer-diagnosis-with-molecular-tools-in-private-gynecological-practice-in-mexico/330897/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/findings-and-challenges-in-replacing-traditional-uterine-cervical-cancer-diagnosis-with-molecular-tools-in-private-gynecological-practice-in-mexico/330897.png","ImageObject",300,407,{"name":92,"@type":93},"Maeve","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-11-11","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":52},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What molecular approach is discussed as an alternative to traditional cervical screening?","Question",{"text":112,"@type":113},"The document emphasizes using PCR-based testing for high-risk HPV genotyping together with cancer biomarkers, instead of relying solely on traditional Papanicolaou and colposcopy workflows.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were cytology aliquots used in the study?",{"text":117,"@type":113},"Liquid-based cytology samples were split into aliquots for HR HPV PCR testing, for the PAP test, and for p16/Ki67 dual-stain cytology.",{"name":119,"@type":110,"acceptedAnswer":120},"What challenges are highlighted when replacing traditional cervical diagnosis in Mexico?",{"text":121,"@type":113},"The paper identifies deep-rooted social reluctance in Mexico to abandon traditional Pap smears and differing opinions among specialists.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},330897,1790128233,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":52,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":41,"preview_page_count":4},5909877438554,"https://ap-avatar.wpscdn.com/avatar/5600025385ad2bf12a7?_k=1778553567797529272","viruses   \nArticle  \nFindings and Challenges in Replacing Traditional Uterine Cervical Cancer Diagnosis with Molecular Tools in Private Gynecological Practice in Mexico  \nJosé L. Castrillo-Diez 1, Carolina Rivera-Santiago 2,3, Silvia M. Ávila-Flores 1, Silvia A. Barrera-Barrera 4 and Hugo A. Barrera-Saldaña 3,4,5, *  \nCitation: Castrillo-Diez, J.L.; Rivera-Santiago, C.; Ávila-Flores, S.M.; Barrera-Barrera, S.A.; Barrera-Saldaña, H.A. Findings and Challenges in Replacing Traditional Uterine Cervical Cancer Diagnosis with Molecular Tools in Private Gynecological Practice in Mexico. Viruses 2024, 16, 887. [https://](https://)[ ](https://)[doi.org/10.3390/v16060887](doi.org/10.3390/v16060887)  \nAcademic Editors: Hossein H. Ashrafi and Mustafa Ozdogan  \nReceived: 11 April 2024  \nRevised: 29 May 2024  \nAccepted: 29 May 2024  \nPublished: 31 May 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Leire Genomic Laboratories, León 37000, Mexico  \n2 Columbia Biotec, Columbia Laboratories, Tlalpan 14090, Mexico; [crivera@gcolumbia.com](crivera@gcolumbia.com)  \n3 Columbia Laboratories, Basic Scientific Research Division, Mexico City 04000, Mexico  \n4 Innbiogem SC/Vitagenesis SA at National Laboratory for Services of Research, Development, and Innovation for the Pharma and Biotech Industries (LANSEIDI) of CONACyT Vitaxentrum Group, Monterrey 64630, Mexico; [silviaabb92@gmail.com](silviaabb92@gmail.com)  \n5 Facultades de Medicina y Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico  \n* [Correspondence: hbarrera@gcolumbia.com](Correspondence: hbarrera@gcolumbia.com)  \nAbstract: We have been encouraging practicing gynecologists to adopt molecular diagnostics tests, PCR, and cancer biomarkers, as alternatives enabled by these platforms, to traditional Papanicolaou and colposcopy tests, respectively. An aliquot of liquid-based cytology was used for the molecular test [high-risk HPV types,(HR HPV)], another for the PAP test, and one more for p16/Ki67 dual-stain cytology. A total of 4499 laboratory samples were evaluated, and we found that 25.1% of low-grade samples and 47.9% of high-grade samples after PAP testing had a negative HR HPV-PCR result. In those cases, reported as Pap-negative, 22.1% had a positive HR HPV-PCR result. Dual staining with p16/Ki67 biomarkers in samples was positive for HR HPV, and 31.7% were also positive for these markers. Out of the PCR results that were positive for any of these HR HPV subtypes, n 68.3%, we did not find evidence for the presence of cancerous cells, highlighting the importance of performing dual staining with p16/Ki67 after PCR to avoid unnecessary colposcopies. The encountered challenges are a deep-rooted social reluctance in Mexico to abandon traditional Pap smears and the opinion of many specialists. Therefore, we still believe that colposcopy continues to be a preferred procedure over the dual-staining protocol.  \nKeywords: uterine cervical cancer screening; HPV genotyping; p16/Ki67 biomarkers; gynecology private practice  \n1. Introduction  \nThe specific detection of the human papillomavirus (HPV) subtype has favored the reduction in the mortality and morbidity rate in women due to cervical cancer (CC) worldwide [1] . This reduction has been achieved due to the widespread use of liquid-based cytology preparations to obtain cervical samples and the implementation of new molecular technologies for HPV genotyping by polymerase chain reaction (PCR) [2–5] .  \nGlobally, CC is the fourth most common cancer in women, with 604,000 new cases in 2020 [5] . About 90% of the 342,000 deaths caused by CC occurred in low-and middleincome countries [6,7] . The ","cbCaivEqH0BMOR6k","https://ap.wps.com/l/cbCaivEqH0BMOR6k","pdf",2895565,12,"English","# Abstract\n# Introduction\n# Materials and Methods\n# Results\n# Discussion\n# Conclusions","[{\"question\":\"What molecular approach is discussed as an alternative to traditional cervical screening?\",\"answer\":\"The document emphasizes using PCR-based testing for high-risk HPV genotyping together with cancer biomarkers, instead of relying solely on traditional Papanicolaou and colposcopy workflows.\"},{\"question\":\"How were cytology aliquots used in the study?\",\"answer\":\"Liquid-based cytology samples were split into aliquots for HR HPV PCR testing, for the PAP test, and for p16/Ki67 dual-stain cytology.\"},{\"question\":\"What challenges are highlighted when replacing traditional cervical diagnosis in Mexico?\",\"answer\":\"The paper identifies deep-rooted social reluctance in Mexico to abandon traditional Pap smears and differing opinions among specialists.\"}]","Findings and Challenges in Replacing Traditional Uterine Cervical Cancer Diagnosis with Molecular Tools in Private Gynecological Practice in Mexico | PDF",1789990647]