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FOS-LCP enhances calcium-binding, digestive stability, and resistance to dietary inhibitors, improving Ca2+ uptake and transport in intestinal in vitro 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was the fructooligosaccharide-Larimichthys crocea peptide copolymer prepared?","Question",{"text":63,"@type":64},"The FOSLCP copolymer was fabricated by wet-heating, combining a fructooligosaccharide component with Larimichthys crocea peptide.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"What benefits does FOS-LCP show for calcium handling?",{"text":68,"@type":64},"FOS-LCP exhibits improved calcium-binding capacity, digestive stability, and resistance to dietary inhibitors, leading to increased Ca2+ uptake and transport in intestinal in vitro models.",{"name":70,"@type":61,"acceptedAnswer":71},"How does FOS-LCP influence bone and fat-related processes in vitro?",{"text":72,"@type":64},"In calcium-present conditions, FOS-LCP promotes osteogenic differentiation and osteogenic marker expression while suppressing adipogenic differentiation, with Wnt/β-catenin activation and inhibition of PPARγ/C/EBPα-driven lipogenesis.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},420960,1791757063,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,100,105,109,114,119,122,126],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & 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the 4th International Congress on Natural Products and Sustainable Development (SNDD 2025)\u003Cbr>Fabrication ofFructooligosaccharide-Larimichthys crocea Peptide Copolymer by Wet-Heating for Enhanced Calcium Delivery and Bone-Lipid Metabolism Regulation\u003Cbr>Chunlei Liu1 | Xiaoping Wu1,2  | Yihang Guo1 | Dan Li1,2\u003Cbr>1College of Marine Science, Ningde Normal University, Ningde, China | 2Fujian Provincial University Engineering Research Center for Deep Processing of Mindong Aquatic Products, Ningde, China\u003Cbr>Correspondence: Xiaoping Wu ([wuxiaoping1216@163.com](wuxiaoping1216@163.com))\u003Cbr>Received: 3 June 2025 | Revised: 9 December 2025 | Accepted: 19 December 2025\u003Cbr>Keywords: adipogenesis | calcium absorption | calcium delivery system | osteogenesis | physicochemical characteristics | wet-heating |  |\n| --- | --- |\n| ABSTRACT\u003Cbr>Calcium delivery systems based on food-derived peptides and polysaccharides offer a promising strategy for improving calcium bioavailability while preserving peptide bioactivity. In this study, a fructooligosaccharide-Larimichthys crocea peptide (FOSLCP) copolymer was fabricated by wet-heating and evaluated as a multifunctional calcium carrier. FOS-LCP showed improved calcium-binding capacity, digestive stability, and resistance to dietary inhibitors, thus increasing Ca2+ uptake and transport in intestinal models in vitro. Bone marrow mesenchymal stem cells isolated from calcium–deficient mice were used to assess bone-lipid regulation in vitro. In the presence of Ca2+, FOS-LCP promoted osteogenic differentiation and osteogenic marker expression, suppressed adipogenic differentiation, activated the Wnt/β-catenin pathway, inhibited PPARγ/C/EBPα-driven lipogenesis, reduced adipokine secretion, and enhanced lipolytic gene expression. These findings indicate that FOS-LCP coordinately enhances calcium absorption and rebalances bone-lipid metabolism at the cellular level, supporting wet-heating-derived peptidepolysaccharide copolymers as promising functional calcium delivery systems. |  |\n| 1 | Introduction | insufficient dietary intake and poor calcium bioavailability (Sun, Zhang, et al. 2020; Wu et al. 2022) . Intestinal anti–nutritional |\n| Calcium is essential for skeletal homeostasis, with approxi- | factors, such as phytates (PA), oxalates (OA), and phosphates |\n| mately 99% of body calcium stored in the bones, which slows | (PPA), readily form insoluble complexes with calcium, thereby |\n| bone mineral density loss and helps prevent bone diseases (Wu et al. 2022; Zhu et al. 2020) . Nevertheless, approximately 51% of | reducing calcium absorption and contributing to bone-related disorders (Wu, Wang, et al. 2023) . Therefore, developing func- |\n| the global population is at risk of calcium deficiency owing to | tional delivery systems that can overcome intestinal absorption |\n| Abbreviations: ACC, acetyl-CoA carboxylase; ALP, alkaline phosphatase; aP2, adipocyte fatty-acid binding protein; ATGL, adipose triglyceride lipase; BGP,β-glycerophosphate; BMSCs, bone marrow mesenchymal stem cells; C/EBPα, CCAAT/enhancer-binding protein α; CaMKII, Ca2+/calmodulin-dependent protein kinase II; Col–I, type I collagen; CPPs, casein phosphopeptides; CPT1, carnitine palmitoyltransferase 1; DEX, dexamethasone; DKK1, dickkopf-1; DMT1, divalent metal transporter-1; FAS, fatty acid synthase; FOS, fructooligosaccharides; GPC, gel permeation chromatography; GSK–3β, glycogen synthase kinase-3β; HSL, hormone-sensitive lipase; IBMX, 3-isobutyl-1-methylxanthine; ICP–OES, inductively coupled plasma-optical emission spectrometry; LCP, Larimichthys crocea peptides; LRP5/6, low-density lipoprotein receptor-related protein 5/6; MMP7, matrix metalloproteinase–7; MW, molecular weight; OA, oxalate; OCN, osteocalcin; OPA, ortho-phthalaldehyde; PA, phytate; PKC, protein kinase C; PPA, phosphate; PPARγ, peroxisome proliferator-activated receptors γ; RT-qPCR, rev","cbCaikYIwPsNupLI","https://ap.wps.com/l/cbCaikYIwPsNupLI","pdf",7272528,17,"English","# Abstract\n# Introduction\n## Calcium bioavailability and skeletal homeostasis\n## Dietary inhibitors and calcium absorption barriers\n## Peptide-polysaccharide copolymers for delivery\n# Methods and Evaluations\n## Calcium binding and digestive stability assessment\n## Intestinal uptake/transport models in vitro\n## Bone-lipid regulation using BMSCs\n# Results and Mechanistic Findings\n## Osteogenic and adipogenic differentiation effects\n## Wnt/β-catenin and PPARγ/C/EBPα pathway modulation\n## Adipokine secretion and lipolytic gene expression\n# Conclusion","[{\"question\":\"How was the fructooligosaccharide-Larimichthys crocea peptide copolymer prepared?\",\"answer\":\"The FOSLCP copolymer was fabricated by wet-heating, combining a fructooligosaccharide component with Larimichthys crocea peptide.\"},{\"question\":\"What benefits does FOS-LCP show for calcium handling?\",\"answer\":\"FOS-LCP exhibits improved calcium-binding capacity, digestive stability, and resistance to dietary inhibitors, leading to increased Ca2+ uptake and transport in intestinal in vitro models.\"},{\"question\":\"How does FOS-LCP influence bone and fat-related processes in vitro?\",\"answer\":\"In calcium-present conditions, FOS-LCP promotes osteogenic differentiation and osteogenic marker expression while suppressing adipogenic differentiation, with Wnt/β-catenin activation and inhibition of PPARγ/C/EBPα-driven lipogenesis.\"}]","Fabrication of Fructooligosaccharide-Larimichthys crocea Peptide Copolymer - Wet-Heating-Derived Calcium Delivery and Bone-Lipid Metabolism Regulation | PDF",1790623293,43]