[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-detail-31106":3,"doc-seo-31106":21},{"code":4,"msg":5,"data":6},0,"success",{"doc_id":7,"user_id":8,"nickname":9,"user_avatar":10,"doc_module":4,"category_id":11,"category_name":12,"doc_title":13,"doc_description":14,"file_id":15,"file_url":16,"file_type":17,"file_size":18,"view_count":4,"is_deleted":4,"is_public":19,"is_downloadable":19,"audit_status":19,"update_tm":20},31106,8796095461564,"Liam","https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d",8,"Research & Report","Expression, Sarkosyl Solubilization, DNase Activity, Purification, and SPR Binding Affinity of Recombinant Diphtheria Toxoid (rCRM197EK) Expressed in Escherichia coli BL21(DE3)","CRM197EK is a diphtheria toxoid derivative engineered with two-point mutations (K51E and E148K) to support vaccine conjugates and drug delivery. To address prior challenges where intracellular expression in Escherichia coli formed inclusion bodies requiring complex refolding, rCRM197EK is produced as inclusion bodies in BL21(DE3) and solubilized using N-lauroyl sarcosine (sarkosyl) to obtain a soluble product without renaturation. The protein is purified by Ni–NTA chromatography, validated by SDS-PAGE and Western blot, and its biological function is examined via DNase activity assays. Binding to anti-diphtheria toxin antibody is quantified using SPR, yielding a reported affinity of −9.2 kcal/mol.","cbCaicVfKXK943vA","https://ap.wps.com/l/cbCaicVfKXK943vA","pdf",1316606,1,1778619692,{"code":4,"msg":22,"data":23},"ok",{"site_id":24,"language":25,"slug":26,"title":13,"keywords":27,"description":14,"schema_data":28,"social_meta":62,"head_meta":64,"extra_data":66,"updated_unix":20},105,"en","expression-sarkosyl-solubilization-dnase-activity-purification-and-spr-binding-affinity-of-recombinant-diphtheria-toxoid-rcrm197ek-expressed-in-escherichia-coli-bl21-de3","",{"@graph":29,"@context":61},[30,47],{"@type":31,"itemListElement":32},"BreadcrumbList",[33,37,41,44],{"item":34,"name":35,"@type":36,"position":19},"https://docshare.wps.com","Home","ListItem",{"item":38,"name":39,"@type":36,"position":40},"https://docshare.wps.com/document/","Document",2,{"item":42,"name":12,"@type":36,"position":43},"https://docshare.wps.com/document/research-report/",3,{"item":45,"name":13,"@type":36,"position":46},"https://docshare.wps.com/document/expression-sarkosyl-solubilization-dnase-activity-purification-and-spr-binding-affinity-of-recombinant-diphtheria-toxoid-rcrm197ek-expressed-in-escherichia-coli-bl21-de3/31106",4,{"url":45,"name":13,"@type":48,"author":49,"headline":13,"publisher":51,"fileFormat":54,"description":14,"dateModified":55,"datePublished":55,"encodingFormat":54,"isAccessibleForFree":56,"interactionStatistic":57},"DigitalDocument",{"name":9,"@type":50},"Person",{"url":34,"name":52,"@type":53},"DocShare","Organization","application/pdf","2026-05-12",true,{"@type":58,"interactionType":59,"userInteractionCount":4},"InteractionCounter",{"@type":60},"ViewAction","https://schema.org",{"og:url":45,"og:type":63,"og:title":13,"og:site_name":52,"og:description":14},"article",{"robots":65,"canonical":45},"index,follow",{"doc_id":7,"site_id":24}]