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0 2 6年1月第2 9卷第1期 Chin J Lung Cancer, January 2 0 2 6 , Vol. 2 9 , No. 1 · 􀀒􀀖 ·  \nDOI: 10.3779/j.issn.1009-3419.2026.106.02 ·临床研究 ·  \n非小细胞肺癌中SRSF家族蛋白的表达和预后及功能分析  \n涂姝祺 陈宇昊 张亚龙 陈嫱 范亚光 王逸璇 张洋 李思诺 陈军 潘红丽 周雪霞 李雪冰  \n【摘要】 背景与目的 非小细胞肺癌（non-small cell lung cancer, NSCLC）是全球癌症相关死亡的主要原因之  \n一，其发生发展与复杂的分子机制密切关联。前体mRNA选择性剪接的异常在肿瘤中普遍存在，丝氨酸/精氨酸富集剪接因子（serine and arginine rich splicing factor, SRSF）家族是该过程的核心调控者。然而， 目前对于SRSF家族在 NSCLC中的系统性研究仍不充分。本研究旨在通过生物信息学分析与实验验证相结合的方式，系统分析SRSF家族在NSCLC中的表达谱、预后价值及其潜在生物学功能。方法 本研究整合癌症基因组图谱（The Cancer Genome Atlas, TCGA）和癌细胞系百科全书（Cancer Cell Line Encyclopedia, CCLE）等公共数据库中的转录组与临床数据，分析SRSF家族在NSCLC中的差异表达。通过Kaplan-Meier生存分析评估其表达水平与患者总生存期的关系。利用基因本体（Gene Ontology, GO）及京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路富集分析探讨其功能。进一步收集临床样本和细胞系，通过逆转录定量聚合酶链反应（reverse transcription quantitative polymerase chain reaction, RT-qPCR）、细胞计数试剂盒-8（cell counting kit-8, CCK-8）及细胞增殖实验验证关键成员的表达与功能。结果 SRSF家族多个成员（如SRSF1、 SRSF2、 SRSF3、 SRSF6、 SRSF7、 SRSF9、 SRSF10等）在NSCLC 组织及细胞系中显著高表达。生存分析表明，SRSF9的高表达与患者不良预后有关，而SRSF11和SRSF12的低表达提示预后较差。功能富集分析揭示，SRSF家族不仅参与RNA剪接，还显著富集于蛋白质稳态、细胞应激反应及神经退行性疾病相关通路。体外实验证实，敲低SRSF1、 SRSF2、 SRSF6、 SRSF9、 SRSF10可显著抑制NSCLC细胞的增殖能力。结论 本研究系统描绘了SRSF家族在NSCLC中的表达与功能图谱，证实了其作为预后生物标志物和治疗靶点的潜力。本研究提示，SRSF家族可能通过破坏细胞稳态（如蛋白质稳态与应激反应）这一在肿瘤发生中至关重要  \n的环节来驱动NSCLC的进展，为深入理解剪接失调在肺癌中的作用及开发新型治疗策略提供了理论依据。  \n【关键词】 肺肿瘤； SRSF家族；生存预后；选择性剪接；生物信息学分析；细胞增殖与活力  \nExpression, Prognostic and Functional Analysis of SRSF Family Proteins  \nin Non-small Cell Lung Cancer  \nShuqi TU1, Yuhao CHEN1,2, Yalong ZHANG3, Qiang CHEN1,4, Yaguang FAN1, Yixuan WANG1,5, Yang ZHANG1, Sinuo LI1, Jun CHEN1, Hongli PAN1, Xuexia ZHOU5, Xuebing LI1  \n1Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin 300052, China; 2Second Department of Thoracic Surgery, Hunan Cancer Hospital, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China; 3Department of Pathology, Binzhou Medical University Hospital, Binzhou 256600, China; 4Department of Respiratory and Critical Medicine, Tianjin Chest Hospital, Tianjin 300202, China; 5Tianjin Neurological Institute, Tianjin Medical University  \nGeneral Hospital, Tianjin 300052, China  \nCorresponding author: Hongli PAN, [E-mail: panhongli@tmu.edu.cn](E-mail: panhongli@tmu.edu.cn);  \nXuexia ZHOU, [E-mail: xxzhou@tmu.edu.cn](E-mail: xxzhou@tmu.edu.cn);  \nXuebing LI, [E-mail: xbli@tmu.edu.cn](E-mail: xbli@tmu.edu.cn)  \n本研究受国家自然科学基金项目（ No. 82273019, No. 82472643）、天津医科大学总医院优秀青年基金项目（ No.22ZYYYQ01）、天津市科技重大专项与工程公共卫生科技重大专项重点项目（No.24ZXGZSY00040）、天津市卫生健康科技基金项目（No.TJWJ2023QN063）和天津市医学重点学科建设项目（ No.TJYXZDXK-3-002B, No.TJYXZDXK-3-006A-2 ）资助  \n作者单位： 300052天津，天津医科大学总医院，天津市肺癌研究所（涂姝祺，陈宇昊，陈嫱，范亚光，王逸璇，张洋，李思诺，陈军，潘红丽，李雪冰）；410013长沙，中南大学湘雅医学院附属肿瘤医院胸外科二病区（陈宇昊）；256600滨州，滨州医学院附属医院病理科（张亚龙）； 300202天津，天津市胸科医院呼吸与危重症医学科（陈嫱）；300052天津，天津医科大学总医院，天津市神经病学研究所（王逸璇，周雪霞）  \n（通信作者：潘红丽， [E-mail: panhongli@tmu.edu.cn](E-mail: panhongli@tmu.edu.cn)；周雪霞， [E-mail: xxzhou@tmu.edu.cn](E-mail: xxzhou@tmu.edu.cn)；李雪冰， [E-mail: xbli@tmu.edu.cn](E-mail: xbli@tmu.edu.cn)）  \n· 􀀒􀀗 · 中国肺癌杂志2 0 2 6年1月第2 9卷第1期 Chin J Lung Cancer, January 2 0 2 6 , Vol. 2 9 , No. 1  \n【Abstract】 Background and objective Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer-related deaths worldwide, and its occurrence and development are closely related to complex molecular mechanisms. Alternative splicing of precursor mRNA is a key step in gene expression regulation, and its dysregulation is common in tumors. The serine/arginine-rich splicing factor (SRSF) family, a core protein family in splicing regulation, has been confirmed to play oncogenic roles in various cancers. However, systematic research on the SRSF family in NSCLC remains insufficient. This study aims to systematically analyze the specific expression patterns, clinical prognostic value, collaborative mechanisms and potential biological functions of SRSF individual members in NSCLC by the combination of bioinformatics analysis and experimental verific","cbCaigI6cNqR5wKu","https://ap.wps.com/l/cbCaigI6cNqR5wKu","pdf",14814381,11,"Chinese","en","# 摘要\n## 背景与目的\n## 方法\n## 结果\n## 结论\n# 关键词","[{\"question\":\"SRSF家族在NSCLC中有哪些表达特征？\",\"answer\":\"多名SRSF家族成员（如SRSF1、SRSF2、SRSF3、SRSF6、SRSF7、SRSF9、SRSF10）在NSCLC组织及细胞系中显著高表达。\"},{\"question\":\"SRSF成员的表达与患者预后有什么关系？\",\"answer\":\"生存分析提示SRSF9高表达与不良预后相关；SRSF11和SRSF12低表达也提示预后较差。\"},{\"question\":\"敲低SRSF家族关键成员对NSCLC细胞有什么影响？\",\"answer\":\"体外实验显示敲低SRSF1、SRSF2、SRSF6、SRSF9和SRSF10可显著抑制NSCLC细胞增殖能力。\"}]","非小细胞肺癌中SRSF家族蛋白的表达和预后及功能分析 | PDF",1790108343,28]