[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-385195-105":59,"doc-detail-385195-en":131},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":124,"head_meta":126,"extra_data":128,"updated_unix":130},105,"en","expression-of-polymeric-immunoglobulin-receptor-pigr-and-the-effect-of-pigr-overexpression-on-breast-cancer-cells","Expression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGR overexpression on breast cancer cells","","Polymeric immunoglobulin receptor (PIGR) is a key component of mucosal immunity, transporting polymeric immunoglobulin across epithelial cells. This study tested whether PIGR influences cellular behaviours and chemo-sensitivity in MCF7 and MDA-MB468 breast cancer cell lines. Basal PIGR mRNA and protein levels were measured by qPCR and Western blotting. Lentiviral tetracycline-induced PIGR overexpression produced stable MCF7/PIGR and MDA-MB468/PIGR lines, followed by viability, proliferation, and chemo-sensitivity analyses versus controls.",{"@graph":69,"@context":123},[70,84,106],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/expression-of-polymeric-immunoglobulin-receptor-pigr-and-the-effect-of-pigr-overexpression-on-breast-cancer-cells/385195/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/expression-of-polymeric-immunoglobulin-receptor-pigr-and-the-effect-of-pigr-overexpression-on-breast-cancer-cells/385195.png","ImageObject",300,407,{"name":92,"@type":93},"Dipper","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-24",true,{"@type":102,"interactionType":103,"userInteractionCount":105},"InteractionCounter",{"@type":104},"ViewAction",14,{"@type":107,"mainEntity":108},"FAQPage",[109,115,119],{"name":110,"@type":111,"acceptedAnswer":112},"What is the main purpose of the study regarding PIGR?","Question",{"text":113,"@type":114},"To determine whether PIGR affects cellular behaviours and chemo-sensitivity in MCF7 and MDA-MB468 breast cancer cell lines.","Answer",{"name":116,"@type":111,"acceptedAnswer":117},"How was PIGR expression quantified in the breast cancer cells?",{"text":118,"@type":114},"Basal and induced PIGR mRNA were measured by real-time quantitative PCR, and protein levels were assessed by Western blotting.",{"name":120,"@type":111,"acceptedAnswer":121},"Does PIGR overexpression change chemo-sensitivity or cellular behaviour in these cell lines?",{"text":122,"@type":114},"No. Although PIGR mRNA and protein were higher in MDA-MB468 than in MCF7, differential expression did not produce significant differences in chemo-sensitivity, and viral overexpression did not alter measured parameters.","https://schema.org",{"og:url":83,"og:type":125,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":127,"canonical":83},"index,follow",{"doc_id":129,"site_id":62},385195,1790472268,{"code":4,"msg":5,"data":132},{"doc_id":129,"user_id":133,"nickname":92,"user_avatar":134,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":105,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":44,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374404997633,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nExpression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGRoverexpression on breast cancer cells  \nWichitra Asanprakit1,2,3, Dileep N. Lobo2,4*, Oleg Eremin2 & Andrew J. Bennett 1  \nPolymeric immunoglobulin receptor (PIGR) has a major role in mucosal immunity as a transporter of polymeric immunoglobulin across the epithelial cells. The aim of this study was to determine the effect of PIGR on cellular behaviours and chemo-sensitivity of MCF7 and MDA-MB468 breast cancer cell lines. Basal levels of PIGR mRNA and protein expression in MCF7 and MDA-MB468 cells were evaluated by real time quantitative polymerase chain reaction and Western blotting, respectively. MCF7/PIGR and MDA-MB468/PIGR stable cell lines, overexpressing the PIGR gene, were generated using a lentiviral vector with tetracycline dependent induction of expression. Cell viability, cell proliferation and chemo-sensitivity of PIGR transfected cells were evaluated and compared with un-transfected cells to determine the effect of PIGR overexpression on cell phenotype. The levels of PIGR mRNA and protein expression were significantly higher in MDA-MB468 cells than in MCF7 cells (380-fold, p \u003C 0.0001) . However, the differential expression of PIGR in these two cell lines did not lead to significant differences in chemosensitivity. Viral overexpression of PIGR was also not found to change any of the parameters measured in either cell line. PIGR per se did not affect cellular behaviours and chemosensitivity of these breast cancer cell lines.  \nAbbreviations  \nANOVA Analysis of variance BrdU Bromodeoxyuridine  \nDMEM Dulbecco’s modified Eagle medium EMEM Eagle’s minimum essential medium EMT Epithelial mesenchymal transition  \nFBS Foetal bovine serum  \nHRP Horseradish peroxidase  \nIFN Interferon  \nIL Interleukin  \nHEK Human embryonic kidney  \nPIGR Polymeric immunoglobulin receptor qPCR Quantitative polymerase chain reaction  \nPolymeric immunoglobulin receptor (PIGR) has a major role in mucosal immunity as a transporter of polymeric immunoglobulin across the epithelial cells. PIGR has been demonstrated and described as a biomarker in many cancers. The level of PIGR expression varies among cancer types and is associated with different patient  \n1FRAME Alternatives Laboratory, Faculty of Medicine and Health Sciences, School of Life Sciences, University of Nottingham, Nottingham, UK. 2Gastrointestinal Surgery, Nottingham Digestive Diseases Centre and National Institute for Health Research (NIHR) Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and University of Nottingham, Queen’s Medical Centre, Nottingham NG7 2UH, UK. 3Department of Surgery, Phramongkutklao Hospital and College of Medicine, Bangkok, Thailand. 4MRC Versus Arthritis Centre for Musculoskeletal Ageing Research, School of Life Sciences, University of Nottingham, Queen’s Medical Centre,  \nNottingham, UK.*[email: Dileep.Lobo@nottingham.ac.uk](email: Dileep.Lobo@nottingham.ac.uk)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \noutcomes and response to chemotherapy. PIGR expression in hepatocellular cancer, colon cancer, pancreatic cancer, osteosarcoma and glioma has been shown to correlate with poor prognosis1–5. In contrast, many studies have reported favourable outcomes with PIGR expression in patients with other cancers, including upper gastrointestinal tract, lung, endometrial, ovarian and breast cancer6–12.  \nPIGR was found to promote cellular transformation and proliferation in hepatocellular carcinoma13. Moreover, increasing the expression of PIGR in hepatocellular carcinoma cells can induce epithelial mesenchymal transition (EMT) of cancer cells and lead to tumour metastasis1. Downregulation of PIGR in pancreatic cancer cells markedly changed cellular morphology and reduced cellular proliferation, adhesion, migration and invasion14. In contrast, downregulat","cbCaiggXE8MiOm7X","https://ap.wps.com/l/cbCaiggXE8MiOm7X","pdf",1217642,"English","# Aim and rationale\n## Experimental design and stable PIGR cell lines\n## PIGR expression assessment\n## Cellular viability, proliferation, and chemo-sensitivity\n# Materials and methods\n## Cell lines\n## RNA isolation and cDNA synthesis","[{\"question\":\"What is the main purpose of the study regarding PIGR?\",\"answer\":\"To determine whether PIGR affects cellular behaviours and chemo-sensitivity in MCF7 and MDA-MB468 breast cancer cell lines.\"},{\"question\":\"How was PIGR expression quantified in the breast cancer cells?\",\"answer\":\"Basal and induced PIGR mRNA were measured by real-time quantitative PCR, and protein levels were assessed by Western blotting.\"},{\"question\":\"Does PIGR overexpression change chemo-sensitivity or cellular behaviour in these cell lines?\",\"answer\":\"No. Although PIGR mRNA and protein were higher in MDA-MB468 than in MCF7, differential expression did not produce significant differences in chemo-sensitivity, and viral overexpression did not alter measured parameters.\"}]","Expression of polymeric immunoglobulin receptor (PIGR) and the effect of PIGR overexpression on breast cancer cells | PDF",1790267296,23]