[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-446383-105":59,"doc-detail-446383-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","expression-of-periostin-and-podoplanin-in-canine-testicular-tumours","Expression of periostin and podoplanin in canine testicular tumours","","Periostin (POSTN) and podoplanin (PDPN) are proteins implicated in malignant tumour biology through intracellular signalling, cell migration, endothelial structure changes, angiogenesis, and carcinogenesis. A retrospective study assessed 186 canine testicular tumours (Leydig cell, Sertoli cell, seminomas) plus 10 normal testes using immunohistochemistry for POSTN, PDPN, and Ki67, with PCR confirming both proteins in frozen tumour samples. Results show no POSTN/PDPN in normal testes and higher expression in neoplastic types.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/expression-of-periostin-and-podoplanin-in-canine-testicular-tumours/446383/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/expression-of-periostin-and-podoplanin-in-canine-testicular-tumours/446383.png","ImageObject",300,407,{"name":92,"@type":93},"Terk","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What proteins were investigated in canine testicular tumours?","Question",{"text":112,"@type":113},"Periostin (POSTN) and podoplanin (PDPN) were investigated, along with Ki67 as a proliferation marker.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were POSTN and PDPN expression levels measured?",{"text":117,"@type":113},"Expression was evaluated immunohistochemically in canine testicular tumours and normal testes, and PCR was also performed on representative frozen samples.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the main findings regarding POSTN and PDPN in normal versus tumour tissue?",{"text":121,"@type":113},"Normal testes were negative for both proteins, while neoplastic tissues showed cytoplasmic positivity for POSTN and PDPN, with stronger immunohistochemical reactions in Sertoli cell tumours and seminomas.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},446383,1790879491,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":52,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":143,"read_time":144,"preview_page_count":4},1099525198933,"https://ap-avatar.wpscdn.com/davatar_155a257f0dc6eb9ab79c44ca47cae57d","Ciaputa etal. BMC Veterinary Research (2026) 22:7  \n[https://doi.org/10.1186/s12917-025-05176-y](https://doi.org/10.1186/s12917-025-05176-y)  \nBMC Veterinary Research  \nRESEARCH Open Access  \nExpression of periostin and podoplaninin canine testicular tumours  \nRafał Ciaputa 1*, Izabela Janus-Ziółkowska 1, Kacper Żebrowski 1, Marcin Nowak 1, Stanisław Dzimira 1, Aleksandra Piotrowska2, Katarzyna Ratajczak-Wielgomas2, Małgorzata Kandefer-Gola 1 and Valeria Grieco3  \nAbstract  \nBackground Periostin (POSTN) and podoplanin (PDPN) are both proteins playing an important role in humans in the diagnosis and study of many malignant tumours. POSTN affects the regulation of intracellular signalling pathways involving protein kinases PI3-K/Akt and focal adhesion kinase, whereas PDPN increases fibroblast migration and affects the structure of the endothelium; both proteins participate in carcinogenesis, by increasing cell migration and intensifying angiogenesis. In veterinary medicine, their expression has been demonstrated in only a few types of neoplasms, however their presence has not been investigated in canine testicular tumours which are common in dogs and represent a reliable animal model for their human counterparts, due to their structure and biological behaviour. In the present retrospective study, 186 canine testicular tumours namely 61 Leydig cell tumours, 64 Sertoli cell tumours and 61 seminomas and 10 normal canine testicles were immunohistochemically tested for expression of POSTN, PDPN and Ki67 antigens. The polymerase chain reaction (PCR) analysis was also performed on stored frozen samples representative of the three tumour types: 10 Leydig cell tumours, 9 Sertoli cell tumours and 9 seminomas, which confirmed the presence of both proteins.  \nResults Normal testes were negative for both POSTN and PDPN, whereas 27 (44 . 3%) Leydig cell tumours, 54 (84 .4%) Sertoli cell tumours and 56 (91 . 8%) seminomas were cytoplasmically positive for POSTN and 23 (37 . 7%),  \n49 (77 . 6%) and 44 (72 . 1%) were cytoplasmically positive for PDPN respectively. The intensity of POSTN and PDPNimmunohistochemical reaction was stronger in Sertoli cell tumours and seminomas than in Leydig cell tumours. In addition, Ki67 antigen expression for both POSTN and PDPN correlated significantly with the number of positive cells and the intensity of the reaction in seminomas and Sertoli cell tumours.  \nConclusions The results showed the absence of expression of POSTN and PDPN in normal canine testes and their expression in neoplastic ones, suggesting a role for these proteins in the carcinogenesis of the testis and encouraging further studies, in particular, on seminomas and Sertoli cell tumours.  \nKeywords Testis, Cancer, Oncology, Pathology, Ki-67, Protein, Dog, Immunohistochemistry  \n*Correspondence:  \nRafał Ciaputa  \n[rafal.ciaputa@upwr.edu.pl](rafal.ciaputa@upwr.edu.pl)  \n1Department of Pathology, Division of Pathomorphology and Veterinary Forensics, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, C. K. Norwida 31, Wrocław  \n50-375, Poland  \n2Division of Histology and Embryology, Department of Human Morphology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland  \n3Department of Veterinary Medicine and Animal Science, University of Milan, via dell’Università 6, Lodi 26900, Italy  \n© The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and","cbCaicctVVUsg5Cj","https://ap.wps.com/l/cbCaicctVVUsg5Cj","pdf",2261918,"English","# Abstract\n## Background\n## Results\n## Conclusions\n# Keywords","[{\"question\":\"What proteins were investigated in canine testicular tumours?\",\"answer\":\"Periostin (POSTN) and podoplanin (PDPN) were investigated, along with Ki67 as a proliferation marker.\"},{\"question\":\"How were POSTN and PDPN expression levels measured?\",\"answer\":\"Expression was evaluated immunohistochemically in canine testicular tumours and normal testes, and PCR was also performed on representative frozen samples.\"},{\"question\":\"What were the main findings regarding POSTN and PDPN in normal versus tumour tissue?\",\"answer\":\"Normal testes were negative for both proteins, while neoplastic tissues showed cytoplasmic positivity for POSTN and PDPN, with stronger immunohistochemical reactions in Sertoli cell tumours and seminomas.\"}]","Expression of periostin and podoplanin in canine testicular tumours | PDF",1790715210,25]