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The specific pathogenesis of HPV-mediated head and neck squamous cell carcinoma (HNSCC) remains uncertain, and the roles of CK7, CK19, and p16 in HPV-mediated OPSCC carcinogenesis are unclear. Methods: Case data and paraffin samples from 69 OPSCC and 40 OSCC patients were analyzed by immunohistochemistry for expression intensity, localization, and protein interrelationships. Results: Distinct CK7/CK19/p16 combinations were observed, with HPV-mediated (p16+) OPSCC showing higher CK7/CK19 positive rates and H-scores than non-HPV-mediated OPSCC and OSCC. Conclusions: CK7, CK19, and p16 may relate to HPV-mediated OPSCC development.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/expression-of-ck7-ck19-and-p16-in-hpv-mediated-oropharyngeal-squamous-cell-carcinoma/331581/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/expression-of-ck7-ck19-and-p16-in-hpv-mediated-oropharyngeal-squamous-cell-carcinoma/331581.png","ImageObject",300,407,{"name":92,"@type":93},"Evangeline","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-26","2026-09-21",true,{"@type":102,"interactionType":103,"userInteractionCount":34},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"What is the study’s main purpose regarding CK7, CK19, and p16?","Question",{"text":112,"@type":113},"To determine whether CK7, CK19, and p16 expression patterns are associated with HPV-mediated OPSCC and how they may interact in its carcinogenesis mechanism.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How were HPV-mediated and non-HPV-mediated OPSCC cases distinguished?",{"text":117,"@type":113},"They were differentiated based on p16 expression, classifying HPV-mediated OPSCC as p16+ and non-HPV-mediated OPSCC as otherwise.",{"name":119,"@type":110,"acceptedAnswer":120},"What were the key findings for CK7 and CK19 expression in HPV-mediated OPSCC?",{"text":121,"@type":113},"CK7/CK19 positive rates and H-scores in HPV-mediated (p16+) OPSCC were significantly higher than those in non-HPV-mediated OPSCC and OSCC (P \u003C 0.05).",{"name":123,"@type":110,"acceptedAnswer":124},"Were CK7, CK19, and p16 expression relationships consistent across OPSCC and OSCC?",{"text":125,"@type":113},"In OPSCC, the expression of CK7 with CK19, CK7 with p16, and CK19 with p16 showed consistency (P \u003C 0.05), while in OSCC only CK7 with CK19 demonstrated consistency (P \u003C 0.05).","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},331581,1790160395,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":34,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":143,"language":144,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":145,"faqs":146,"seo_title":147,"seo_description":67,"update_tm":148,"read_time":149,"preview_page_count":4},13056703019662,"https://ap-avatar.wpscdn.com/avatar/be000253a8e92610077?_k=1778726343310543188","Submitted 20 February 2024 Accepted 19 September 2024 Published 28 November 2024  \nCorresponding authors Xiaojuan Zeng, [xiaojuan.zeng@gxmu.edu.cn](xiaojuan.zeng@gxmu.edu.cn)[ ](xiaojuan.zeng@gxmu.edu.cn)Mengyu Zhou, [zhoumengyu@gxmu.edu.cn](zhoumengyu@gxmu.edu.cn)  \nAcademic editor Katherine Mitsouras  \nAdditional Information and Declarations can be found on page 17  \nDOI 10.7717/peerj.18286  \nCopyright 2024 Yan et al.  \nDistributed under  \nCreative Commons CC-BY 4.0  \nExpression of CK7, CK19 and p16 in HPV-mediated oropharyngeal squamous cell carcinoma  \nQizhang Yan 1,2,3,4,5,6, Pengning Chen4,5,6,7, Xinyu Chen4,5,6,7, Guanxi Chen4,5,6,7, Lin Luo4,5,6,7, Ping Ruan8, Dahai Yu9, Xiaojuan Zeng 1,2,3 and Mengyu Zhou9  \n1 Department of Dental Public Health, Guangxi Medical University College of Stomatology, Nanning, China  \n2 Department of Oral Health Policy Research, Guangxi Medical University College ofStomatology, Nanning, China  \n3 Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, China  \n4 Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Nanning, China  \n5 Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, China  \n6 Key Laboratory of Research and Application of Stomatological Equipment, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, China  \n7 Department of Oral and Maxillofacial Surgery, Guangxi Medical University College of Stomatology, Nanning, China  \n8 Department of Pathology, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning, China  \n9 Department of Stomatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China  \nABSTRACT  \nBackground: The incidence of oropharyngeal squamous cell carcinoma (OPSCC) mediated by human papilloma virus (HPV) has been steadily increasing worldwide. The specific pathogenesis of HPV-mediated head and neck squamous cell carcinoma (HNSCC) usually induces carcinogenesis in the oropharynx and the roles of CK7, CK19 and p16 in the carcinogenesis mechanism of HPV-mediated OPSCC still remain uncertain.  \nMethods: We collected case data and paraffin samples of 69 cases of OPSCC and 40 cases of OSCC from July 2009 to December 2021 . Immunohistochemistry was performed on serial paraffin sections from all cases to analyze the expression patterns of CK7, CK19, and p16 . HPV-mediated (p16+) and non-HPV-mediated OPSCC were differentiated based on p16 expression. Three to six fields were selected from each case for observation of the expression intensity, localization, and  \ninterrelationship of the three proteins.  \nResults: In both cancerous nests and pericancerous normal epithelium of OPSCC, various expression combinations of CK7, CK19 and p16 were observed, including CK7+CK19+p16+, CK7−CK19+p16+, and CK7+CK19+p16−, while no expression of CK7+CK19−p16+ was found. The expression of CK7 with CK19, CK7 with p16, and CK19 with p16 all showed consistency in OPSCC (P \u003C 0.05) while only the expression of CK7 and CK19 demonstrated consistency in OSCC (P \u003C 0.05) . The positive rates and H-scores for CK7/CK19 in HPV-mediated (p16+) OPSCC were significantly higher than those in non-HPV-mediated OPSCC and OSCC (P \u003C 0.05) .  \nHow to cite this article Yan Q, Chen P, Chen X, Chen G, Luo L, Ruan P, Yu D, ZengX, Zhou M. 2024. Expression of CK7, CK19 and p16 in HPV-mediated oropharyngeal squamous cell carcinoma. PeerJ 12:e18286 DOI 10.7717/peerj.18286  \nConclusions: These results suggest that CK7, CK19 and p16 may relate to HPV-mediated OPSCC. The interaction of CK7, CK19 and p16 may affect the development of HPV-mediated OPSCC.  \nSubjects Biochemistry, Cell Biology, Oncology, Pathology  \nKeywords HPV, OPSCC, p16, CK7, CK19 INTRODUCTION  \nOropharyngeal squamous cell carcinoma (OPSCC) is a squamous differentiated carcinoma deriving from the oropharyngeal mucosal epithelium, arising from the soft palate, base of the tongue (the po","cbCaiarDsoVCe8Yt","https://ap.wps.com/l/cbCaiarDsoVCe8Yt","pdf",6864527,21,"English","# Abstract\n## Background\n## Methods\n## Results\n## Conclusions\n# Introduction","[{\"question\":\"What is the study’s main purpose regarding CK7, CK19, and p16?\",\"answer\":\"To determine whether CK7, CK19, and p16 expression patterns are associated with HPV-mediated OPSCC and how they may interact in its carcinogenesis mechanism.\"},{\"question\":\"How were HPV-mediated and non-HPV-mediated OPSCC cases distinguished?\",\"answer\":\"They were differentiated based on p16 expression, classifying HPV-mediated OPSCC as p16+ and non-HPV-mediated OPSCC as otherwise.\"},{\"question\":\"What were the key findings for CK7 and CK19 expression in HPV-mediated OPSCC?\",\"answer\":\"CK7/CK19 positive rates and H-scores in HPV-mediated (p16+) OPSCC were significantly higher than those in non-HPV-mediated OPSCC and OSCC (P \\u003c 0.05).\"},{\"question\":\"Were CK7, CK19, and p16 expression relationships consistent across OPSCC and OSCC?\",\"answer\":\"In OPSCC, the expression of CK7 with CK19, CK7 with p16, and CK19 with p16 showed consistency (P \\u003c 0.05), while in OSCC only CK7 with CK19 demonstrated consistency (P \\u003c 0.05).\"}]","Expression of CK7, CK19 and p16 in HPV-mediated oropharyngeal squamous cell carcinoma | PDF",1789994181,53]