[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-329094-105":3,"detail-sidebar-cat-0-en-105":81,"doc-detail-329094-en":131},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":74,"head_meta":76,"extra_data":78,"updated_unix":80},105,"en","expression-dna-methylation-pattern-and-transcription-factor-epb41l3-in-gastric-cancer-a-study-of-262-cases","Expression, DNA methylation pattern and transcription factor EPB41L3 in gastric cancer - a study of 262 cases","","The study investigates EPB41L3 expression and DNA methylation mechanisms in gastric cancer, focusing on how methylation can inactivate tumor suppressor function and drive oncogenesis. Immunohistochemistry shows DAL-1 expression is markedly reduced in tumor tissues and that downregulation is an independent prognostic marker. High-throughput bisulfite sequencing links higher non-CpG promoter methylation with malignant progression and higher-grade classification, diminishing DAL-1 expression. Functional assays identify CDC5L binding to the EPB41L3 promoter, and RNA-seq reveals downstream extracellular matrix and space pathways, with AREG and COL17A1 upregulated after demethylation or overexpression.",{"@graph":14,"@context":73},[15,34,56],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/research-report/","Research & Report",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/expression-dna-methylation-pattern-and-transcription-factor-epb41l3-in-gastric-cancer-a-study-of-262-cases/329094/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/expression-dna-methylation-pattern-and-transcription-factor-epb41l3-in-gastric-cancer-a-study-of-262-cases/329094.png","ImageObject",300,407,{"name":42,"@type":43},"Rowan","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-11-01","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":55},"InteractionCounter",{"@type":54},"ViewAction",12,{"@type":57,"mainEntity":58},"FAQPage",[59,65,69],{"name":60,"@type":61,"acceptedAnswer":62},"What is the main purpose of this research on EPB41L3 in gastric cancer?","Question",{"text":63,"@type":64},"The research aims to clarify EPB41L3 expression and DNA methylation patterns in gastric cancer, identify regulatory transcription factors, and determine the downstream pathways affected by these changes.","Answer",{"name":66,"@type":61,"acceptedAnswer":67},"How does non-CpG methylation of the EPB41L3 promoter relate to gastric cancer progression?",{"text":68,"@type":64},"Higher non-CpG methylation in the EPB41L3 promoter correlates with more malignant tumor progression and higher tissue grade. This hypermethylation reduces DAL-1 expression, contributing to gastric cancer malignancy.",{"name":70,"@type":61,"acceptedAnswer":71},"Which transcription factor is identified as binding to the EPB41L3 promoter?",{"text":72,"@type":64},"CDC5L is identified as directly binding to the upstream region of the EPB41L3 promoter, confirmed by ChIP-qPCR and luciferase reporter assays, and supported by the correlation between CDC5L and DAL-1 protein levels.","https://schema.org",{"og:url":32,"og:type":75,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":77,"canonical":32},"index,follow",{"doc_id":79,"site_id":7},329094,1790838428,{"code":4,"msg":82,"data":83},"success",[84,88,92,96,101,106,111,115,120,123,127],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":85,"show_sort_weight":86,"slug":87},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":93,"show_sort_weight":94,"slug":95},"Exam",70,"exam",{"id":97,"doc_module":4,"doc_module_name":25,"category_name":98,"show_sort_weight":99,"slug":100},5,"Comic",60,"comic",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},6,"Technology",50,"technology",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},7,"Healthcare",40,"healthcare",{"id":112,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":113,"slug":114},8,30,"research-report",{"id":116,"doc_module":4,"doc_module_name":25,"category_name":117,"show_sort_weight":118,"slug":119},9,"Religion & Spirituality",20,"religion-spirituality",{"id":118,"doc_module":4,"doc_module_name":25,"category_name":121,"show_sort_weight":118,"slug":122},"World Cup","world-cup",{"id":124,"doc_module":4,"doc_module_name":25,"category_name":125,"show_sort_weight":124,"slug":126},10,"Lifestyle","lifestyle",{"id":128,"doc_module":4,"doc_module_name":25,"category_name":129,"show_sort_weight":97,"slug":130},19,"General","general",{"code":4,"msg":82,"data":132},{"doc_id":79,"user_id":133,"nickname":42,"user_avatar":134,"doc_module":4,"category_id":112,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":135,"file_id":136,"file_url":137,"file_type":138,"file_size":139,"view_count":55,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":140,"language":141,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":142,"faqs":143,"seo_title":144,"seo_description":12,"update_tm":145,"read_time":146,"preview_page_count":4},1099514067415,"https://ap-avatar.wpscdn.com/avatar/100002539d78ffe74a7?x-image-process=image/resize,m_fixed,w_180,h_180&k=1779092875211072502","Cai etal. Cell Communication and Signaling (2024) 22:470  \n[https://doi.org/10.1186/s12964-024-01849-7](https://doi.org/10.1186/s12964-024-01849-7)  \nCell Communication and Signaling  \nRESEARCH Open Access  \nExpression, DNA methylation pattern and transcription factor EPB41L3 in gastric cancer: a study of 262 cases  \nMengdi Cai 1,2†, Haonan Guo 1†, Dong Wang 1, Tie Zhao 1, Xiao Liang2, Jiaqi Li2, XiaoBo Cui2, Songbin Fu2 and Jingcui Yu 1,2*  \nAbstract  \nPurpose DNA methylation prominently inactivates tumor suppressor genes and facilitates oncogenesis. Previously, we delineated a chromosome 18 deletion encompassing the erythrocyte membrane protein band 4. 1-like 3 (EPB41L3) gene, a progenitor for the tumor suppressor that is differential ly expressed in adenocarcinoma of the lung-1 (DAL-1) in gastric cancer (GC) .  \nMethods Our current investigation aimed to elucidate EPB41L3 expression and methylation in GC, identify regulatory transcription factors, and identify affected downstream pathways. Immunohistochemistry demonstrated that DAL-1 expression is markedly reduced in GC tissues, with its downregulation serving as an independent prognostic marker. Results High-throughput bisulfite sequencing of 70 GC patient tissue pairs revealed that higher methylation of non-CpGs in the EPB41L3 promoter was correlated with more malignant tumor progression and higher-grade tissue classification. Such hypermethylation was shown to diminish DAL-1 expression, thus contributing to the malignancy of GC phenotypes. The DNA methyltransferase inhibitor 5-aza-2’-deoxycytidine (5-aza-CdR) was found to partially restore DAL-1 expression. Moreover, direct binding of the transcription factor CDC5L to the upstream region of the EPB41L3 promoter was identified via chromosome immunoprecipitation (ChIP)-qPCR and luciferase reporter assays. Immunohistochemistry confirmed the positive correlation between CDC5L and DAL-1 protein levels. Subsequent RNA-seq analysis revealed that DAL-1 significantly influences the extracellular matrix and space-related pathways.  \nGC cell RNA-seq post-5-Aza-CdR treatment and single-cell RNA-seq data of GC tissues confirmed the upregulation of AREG and COL17A1, pivotal tumor suppressors, in response to EPB41L3 demethylation or overexpression in GC epithelial cells.  \nConclusion In conclusion, this study elucidates the association between non-CpG methylation of EPB41L3 and GC progression and identifies the key transcription factors and downstream molecules involved. These findings enhance our understanding of the role of EPB41L3 in gastric cancer and provide a solid theoretical foundation for future research and potential clinical applications.  \n†Mengdi Cai and Haonan Guo contributed equally to this work.  \n*Correspondence:  \nJingcui Yu[yujingcui@ems.hrbmu.edu.cn](yujingcui@ems.hrbmu.edu.cn)  \nFull list of author information is available at the end of the article  \n© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativecommons.org/l)icenses/by-nc-n","cbCaiet6sQhuO1FD","https://ap.wps.com/l/cbCaiet6sQhuO1FD","pdf",7887870,14,"English","# Abstract\n# Plain English summary\n# Introduction\n## DNA methylation in cancer pathogenesis\n# Methods\n# Results\n# Conclusion","[{\"question\":\"What is the main purpose of this research on EPB41L3 in gastric cancer?\",\"answer\":\"The research aims to clarify EPB41L3 expression and DNA methylation patterns in gastric cancer, identify regulatory transcription factors, and determine the downstream pathways affected by these changes.\"},{\"question\":\"How does non-CpG methylation of the EPB41L3 promoter relate to gastric cancer progression?\",\"answer\":\"Higher non-CpG methylation in the EPB41L3 promoter correlates with more malignant tumor progression and higher tissue grade. This hypermethylation reduces DAL-1 expression, contributing to gastric cancer malignancy.\"},{\"question\":\"Which transcription factor is identified as binding to the EPB41L3 promoter?\",\"answer\":\"CDC5L is identified as directly binding to the upstream region of the EPB41L3 promoter, confirmed by ChIP-qPCR and luciferase reporter assays, and supported by the correlation between CDC5L and DAL-1 protein levels.\"}]","Expression, DNA methylation pattern and transcription factor EPB41L3 in gastric cancer - a study of 262 cases | PDF",1789982411,35]