[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"doc-seo-331997-105":3,"detail-sidebar-cat-0-en-105":72,"doc-detail-331997-en":122},{"code":4,"msg":5,"data":6},0,"ok",{"site_id":7,"language":8,"slug":9,"title":10,"keywords":11,"description":12,"schema_data":13,"social_meta":65,"head_meta":67,"extra_data":69,"updated_unix":71},105,"en","exploring-the-therapeutic-effects-of-atractylodes-macrocephala-koidz-against-human-gastric-cancer-research-article","Exploring the Therapeutic Effects of Atractylodes macrocephala Koidz against Human Gastric Cancer - Research Article","","Atractylodes macrocephala Koidz (AMK) is a traditional herbal medicine used across East Asia for long-term management of diverse illnesses, including cancers. This study assessed the anti-cancer potential of AMK extract on apoptosis and tumor-grafted mice using AGS human gastric adenocarcinoma cells. Mechanistic analyses combined systems pharmacology via the TCMSP database with multiple cellular and in vivo assays. AMK extract reduced AGS viability, increased sub-G1 fraction, depolarized mitochondria, elevated ROS, activated caspases, modulated MAPK signaling, and suppressed migration, spheroid growth, and xenograft tumor growth.",{"@graph":14,"@context":64},[15,34,55],{"@type":16,"itemListElement":17},"BreadcrumbList",[18,23,27,31],{"item":19,"name":20,"@type":21,"position":22},"https://docshare.wps.com","Home","ListItem",1,{"item":24,"name":25,"@type":21,"position":26},"https://docshare.wps.com/document/","Document",2,{"item":28,"name":29,"@type":21,"position":30},"https://docshare.wps.com/document/healthcare/","Healthcare",3,{"item":32,"name":10,"@type":21,"position":33},"https://docshare.wps.com/document/exploring-the-therapeutic-effects-of-atractylodes-macrocephala-koidz-against-human-gastric-cancer-research-article/331997/",4,{"url":32,"name":10,"@type":35,"image":36,"author":41,"headline":10,"publisher":44,"fileFormat":47,"inLanguage":8,"description":12,"dateModified":48,"datePublished":49,"encodingFormat":47,"isAccessibleForFree":50,"interactionStatistic":51},"DigitalDocument",{"url":37,"@type":38,"width":39,"height":40},"https://docshare.wps.com/thumbnails/exploring-the-therapeutic-effects-of-atractylodes-macrocephala-koidz-against-human-gastric-cancer-research-article/331997.png","ImageObject",300,407,{"name":42,"@type":43},"Olivia Brown","Person",{"url":19,"name":45,"@type":46},"DocShare","Organization","application/pdf","2026-10-22","2026-09-21",true,{"@type":52,"interactionType":53,"userInteractionCount":33},"InteractionCounter",{"@type":54},"ViewAction",{"@type":56,"mainEntity":57},"FAQPage",[58],{"name":59,"@type":60,"acceptedAnswer":61},"How did the researchers identify AMK-related bioactive targets and pathways?","Question",{"text":62,"@type":63},"They used a network-based systems pharmacology approach leveraging the Traditional Chinese Medical Systems Pharmacy (TCMSP) database to link herb compounds with target genes and associated diseases.","Answer","https://schema.org",{"og:url":32,"og:type":66,"og:title":10,"og:site_name":45,"og:description":12},"article",{"robots":68,"canonical":32},"index,follow",{"doc_id":70,"site_id":7},331997,1790434206,{"code":4,"msg":73,"data":74},"success",[75,79,83,87,92,97,101,106,111,114,118],{"id":22,"doc_module":4,"doc_module_name":25,"category_name":76,"show_sort_weight":77,"slug":78},"Story & Novel",90,"story-novel",{"id":26,"doc_module":4,"doc_module_name":25,"category_name":80,"show_sort_weight":81,"slug":82},"Literature",80,"literature",{"id":33,"doc_module":4,"doc_module_name":25,"category_name":84,"show_sort_weight":85,"slug":86},"Exam",70,"exam",{"id":88,"doc_module":4,"doc_module_name":25,"category_name":89,"show_sort_weight":90,"slug":91},5,"Comic",60,"comic",{"id":93,"doc_module":4,"doc_module_name":25,"category_name":94,"show_sort_weight":95,"slug":96},6,"Technology",50,"technology",{"id":98,"doc_module":4,"doc_module_name":25,"category_name":29,"show_sort_weight":99,"slug":100},7,40,"healthcare",{"id":102,"doc_module":4,"doc_module_name":25,"category_name":103,"show_sort_weight":104,"slug":105},8,"Research & Report",30,"research-report",{"id":107,"doc_module":4,"doc_module_name":25,"category_name":108,"show_sort_weight":109,"slug":110},9,"Religion & Spirituality",20,"religion-spirituality",{"id":109,"doc_module":4,"doc_module_name":25,"category_name":112,"show_sort_weight":109,"slug":113},"World Cup","world-cup",{"id":115,"doc_module":4,"doc_module_name":25,"category_name":116,"show_sort_weight":115,"slug":117},10,"Lifestyle","lifestyle",{"id":119,"doc_module":4,"doc_module_name":25,"category_name":120,"show_sort_weight":88,"slug":121},19,"General","general",{"code":4,"msg":73,"data":123},{"doc_id":70,"user_id":124,"nickname":42,"user_avatar":125,"doc_module":4,"category_id":98,"category_name":29,"doc_title":10,"doc_description":12,"doc_content":126,"file_id":127,"file_url":128,"file_type":129,"file_size":130,"view_count":33,"is_deleted":4,"is_public":22,"is_downloadable":22,"audit_status":22,"page_count":131,"language":132,"language_code":8,"site_id":7,"html_lang":8,"table_of_contents":133,"faqs":134,"seo_title":135,"seo_description":12,"update_tm":136,"read_time":137},16904993612988,"https://ap-avatar.wpscdn.com/davatar_a8503ba1806abce46bf441b54a3ca4cd","nutrients   \nArticle  \nExploring the Therapeutic Effects ofAtractylodes macrocephala Koidz against Human Gastric Cancer  \nNa-Ri Choi 1, Woo-Gyun Choi 1, Anlin Zhu 2, Joon Park 3,4, Yun-Tai Kim 3,4, Jaewoo Hong 2, * and Byung-Joo Kim 1, *  \nCitation: Choi, N.-R.; Choi, W.-G.; Zhu, A.; Park, J.; Kim, Y.-T.; Hong, J.; Kim, B.-J. Exploring the Therapeutic Effects of Atractylodes macrocephala Koidz against Human Gastric Cancer. Nutrients 2024, 16, 965. [https://](https://)[ ](https://)[doi.org/10.3390/nu16070965](doi.org/10.3390/nu16070965)  \nAcademic Editor: Rosa Casas  \nReceived: 4 March 2024  \nRevised: 18 March 2024  \nAccepted: 23 March 2024  \nPublished: 27 March 2024  \nCopyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) .  \n1 Department of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 50612, Republic of Korea; [nariring@gmail.com](nariring@gmail.com) (N.-R.C.); [ak0510@hanmail.net](ak0510@hanmail.net) (W.-G.C.)  \n2 Department of Physiology, Daegu Catholic University School of Medicine, Daegu 42472, Republic of Korea; [zhuanlin@cu.ac.kr](zhuanlin@cu.ac.kr)  \n3 Division of Food Functionality, Korea Food Research Institute, Wanju-gun 55365, Republic of Korea; [biosciencepark@gmail.com](biosciencepark@gmail.com) (J.P.); [ytkim@kfri.re.kr](ytkim@kfri.re.kr) (Y.-T.K.)  \n4 Department of Food Biotechnology, Korea University of Science & Technology, Daejeon 34113, Republic of Korea  \n* [Correspondence: jhong@cu.ac.kr](Correspondence: jhong@cu.ac.kr) (J.H.); [vision@pusan.ac.kr](vision@pusan.ac.kr) (B.-J.K.)  \nAbstract: Atractylodes macrocephala Koidz (AMK) is a traditional herbal medicine used for thousands of years in East Asia to improve a variety of illnesses and conditions, including cancers. This study explored the effect of AMK extract on apoptosis and tumor-grafted mice using AGS human gastric adenocarcinoma cells. We investigated the compounds, target genes, and associated diseases of AMK using the Traditional Chinese Medical Systems Pharmacy (TCMSP) database platform. Cell viability assay, cell cycle and mitochondrial depolarization analysis, caspase activity assay, reactive oxygen species (ROS) assay, and wound healing and spheroid formation assay were used to investigate the anti-cancer effects of AMK extract on AGS cells. Also, in vivo studies were conducted using subcutaneous xenografts. AMK extract reduced the viability of AGS cells and increased the sub-G1 cell fraction and the mitochondrial membrane potential. Also, AMK extract increased the production of ROS. AMK extract induced the increased caspase activities and modulated the mitogen-activated protein kinases (MAPK) . In addition, AMK extract effectively inhibited AGS cell migration and led toa notable reduction in the growth of AGS spheroids. Moreover, AMK extract hindered the growth of AGS xenograft tumors in NSG mice. Our results suggest that AMK has anti-cancer effects by promoting cell cycle arrest and inhibiting the proliferation of AGS cancer cells and a xenograft model through apoptosis. This study could provide a novel approach to treat gastric cancer.  \nKeywords: Atractylodes macrocephala Koidz; traditional herbal medicine; AGS human gastric adenocarcinoma cell; anti-cancer; xenograft  \n1. Introduction  \nGastric cancer ranks as the fifth most common cancer in world [1,2] . Without early detection, it is challenging to treat cancer, and patients and their families experience excruciating pain and severe financial losses, resulting in significant social suffering [3] . As the emphasis shifts from mere life extension to improving the quality of life, including overall health and well-being, there is a growing ","cbCaiaG1ZcZ1LBph","https://ap.wps.com/l/cbCaiaG1ZcZ1LBph","pdf",5695988,23,"English","# Introduction\n## Rationale for gastric cancer and need for new therapies\n## Background on AMK and apoptosis in cancer therapy\n## Study approach: systems pharmacology and TCMSP","[{\"question\":\"How did the researchers identify AMK-related bioactive targets and pathways?\",\"answer\":\"They used a network-based systems pharmacology approach leveraging the Traditional Chinese Medical Systems Pharmacy (TCMSP) database to link herb compounds with target genes and associated diseases.\"}]","Exploring the Therapeutic Effects of Atractylodes macrocephala Koidz against Human Gastric Cancer - Research Article | PDF",1789996217,58]