[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-353544-105":59,"doc-detail-353544-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","exploring-the-molecular-function-of-lypd3-from-pan-cancer-to-lung-cancer-based-on-bioinformatics-and-cellular-experiments","Exploring the molecular function of LYPD3 from pan-cancer to lung cancer - based on bioinformatics and cellular experiments","","Malignant tumors pose a major threat to human health, and identifying biomarkers that support early diagnosis and prognosis is an urgent public-health need. This study assesses LYPD3 expression across pan-cancer, evaluates prognostic and diagnostic performance, and links LYPD3 to immune microenvironment features, genetic variation, and antitumor drug sensitivity. RT-PCR and western blot quantify expression after transfection, while flow cytometry, Transwell assays, and apoptosis/invasion markers (Bax, Bcl-2, E-cadherin, N-cadherin, Vimentin) define functional effects.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/exploring-the-molecular-function-of-lypd3-from-pan-cancer-to-lung-cancer-based-on-bioinformatics-and-cellular-experiments/353544/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/exploring-the-molecular-function-of-lypd3-from-pan-cancer-to-lung-cancer-based-on-bioinformatics-and-cellular-experiments/353544.png","ImageObject",300,407,{"name":92,"@type":93},"Finn","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-31","2026-09-22",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What does the study evaluate regarding LYPD3 in pan-cancer?","Question",{"text":112,"@type":113},"The study investigates LYPD3 expression levels and its prognostic and diagnostic values across pan-cancer, along with associations with immune microenvironment, genetic variation, and antitumor drug sensitivity.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How are LYPD3 expression and functional effects measured in cellular experiments?",{"text":117,"@type":113},"RT-PCR and western blot measure transfection efficiency and expression changes, while flow cytometry assesses apoptosis and Transwell assays assess cell invasion and migration. Western blot also evaluates Bax, Bcl-2, E-cadherin, N-cadherin, and Vimentin.",{"name":119,"@type":110,"acceptedAnswer":120},"What is the relationship between LYPD3 and drug sensitivity in the dataset analyses?",{"text":121,"@type":113},"GDSC and CTRP analyses indicate that LYPD3 expression is negatively correlated with drug sensitivity to lapatinib, erlotinib, and afatinib.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},353544,1790228722,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},34359740700684,"https://ap-avatar.wpscdn.com/avatar/1f400023980c374ae676?_k=1777273430885731487","Exploring the molecular function of LYPD3 from pan-cancer to lung cancer: based on bioinformatics and cellular experiments  \nYuan-Xiang Shi1 · Jian-Hua Yan1 · Peng-Hui Dai1  \nReceived: 6 August 2025 / Accepted: 10 January 2026 © The Author(s) 2026  \nAbstract  \nAs a major disease that seriously threatens human health, the prevention and control of malignant tumors has become an important challenge for global public health. In this study, we first investigated the expression level, prognostic value, and diagnostic value of LYPD3 in pan-cancer, and its effects on the immune microenvironment, genetic variation, and antitumor drug sensitivity. RT-PCR and western blot were used to detect the transfection efficiency. Cell apoptosis was detected by flow cytometry. Transwell assay for detection of cell invasion and migration. Western blot was used to detect the expression of Bax, Bcl-2, E-cadherin, N-cadherin and Vimentin. LYPD3 showed high expression in lung cancer, both at the mRNA and protein level. Single-cell transcriptome and spatial transcriptomics reveal that LYPD3 is highly expressed in tumor cells and tumor-associated fibroblasts. ROC analysis revealed that LYPD3 exhibited significant diagnostic efficacy in pan-cancer, and Cox regression analysis revealed that LYPD3 expression was significantly associated with the risk of death in certain cancer types. LYPD3 has a good diagnostic value and prognostic predictive value in pan-cancer. GDSC and CTRP data analysis confirmed that LYPD3 expression was negatively correlated with the drug sensitivity of lapatinib, erlotinib, and afatinib. Focusing on the expression of LYPD3 in lung cancer, cellular experiments revealed that LYPD3 promotes lung cancer cell invasion and metastasis while inhibiting apoptosis. Meanwhile, LYPD3 has a good diagnostic value and prognostic prediction value.  \nIntroduction  \nAs a major disease that seriously threatens human health, the prevention and control of malignant tumors has become an important challenge for global public health (Siegel et al. 2025; Cao et al. 2022) . In recent years, malignant tumor diagnosis and treatment have made breakthrough progress in the direction of precision and individualization (Hill et al. 2024; Zhou et al. 2024) . Liquid biopsy combined with AI imaging histology significantly improves early diagnostic sensitivity. Molecular typing-driven iterations of targeted therapies such as KRAS, bispecific antibodies (PD-1/CTLA-4 dual antibody) and ADC drugs (DS-8201) have expanded treatment options for refractory tumors. In immunotherapy, CAR-T cell combination with immune  \n􀀍 Yuan-Xiang Shi [yuanxiangshi@hunnu.edu.cn](yuanxiangshi@hunnu.edu.cn)  \n1 Institute of Clinical Medicine, Hunan Provincial People’s Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha, China  \ncheckpoint inhibitors are breaking through the bottlenecktherapy is achieving long-term remission in hematologic tumors, while neoantigen vaccines in in solid tumors. In addition, multi-omics integration and single-cell sequencing technologies accurately analyze tumor heterogeneity and microenvironmental interactions, guiding dynamic therapeutic regimen adjustment. However, tumor metastasis, drug resistance, and drug toxicity remain clinical challenges. Therefore, finding new markers for early diagnosis and prognostic prediction of tumors, and probing the molecular mechanisms of tumor development and drug tolerance are urgent scientific problems.  \nLY6/PLAUR domain containing 3 (LYPD3) belongs to the LY6/uPAR protein superfamily, whose members all contain the LY6/uPAR structural domain (a conserved motif of about 80 amino acids containing 8–10 cysteines forming adisulfide bond) and are anchored to the cell membrane by glycosylphosphatidylinositol (GPI) (Loughner et al. 2016; Zaigraev et al. 2022) . Its family members include LYPD1, LYPD3, LYPD5, which are widely involved in cell adhesion, signaling and immune regulation. Existing studies have show","cbCaiasWIkeeEcOd","https://ap.wps.com/l/cbCaiasWIkeeEcOd","pdf",6564237,15,"English","# Abstract\n# Introduction\n# Materials and methods","[{\"question\":\"What does the study evaluate regarding LYPD3 in pan-cancer?\",\"answer\":\"The study investigates LYPD3 expression levels and its prognostic and diagnostic values across pan-cancer, along with associations with immune microenvironment, genetic variation, and antitumor drug sensitivity.\"},{\"question\":\"How are LYPD3 expression and functional effects measured in cellular experiments?\",\"answer\":\"RT-PCR and western blot measure transfection efficiency and expression changes, while flow cytometry assesses apoptosis and Transwell assays assess cell invasion and migration. Western blot also evaluates Bax, Bcl-2, E-cadherin, N-cadherin, and Vimentin.\"},{\"question\":\"What is the relationship between LYPD3 and drug sensitivity in the dataset analyses?\",\"answer\":\"GDSC and CTRP analyses indicate that LYPD3 expression is negatively correlated with drug sensitivity to lapatinib, erlotinib, and afatinib.\"}]","Exploring the molecular function of LYPD3 from pan-cancer to lung cancer - based on bioinformatics and cellular experiments | PDF",1790105980,38]