[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-427704-105":59,"doc-detail-427704-en":134},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":127,"head_meta":129,"extra_data":131,"updated_unix":133},105,"en","exploring-acetyl-coa-mediated-beta-hydroxybutyrate-enrichment-in-yeast-a-mechanistic-link-to-longevity","EXPLORING ACETYL-COA-MEDIATED BETA-HYDROXYBUTYRATE ENRICHMENT IN YEAST - A MECHANISTIC LINK TO LONGEVITY","","Investigates β-hydroxybutyrate (βHB) as a ketone body involved in caloric restriction (CR)-mediated longevity using Saccharomyces cerevisiae. Although canonical ketogenesis enzymes are absent in yeast, βHB is detected in stationary-phase and CR conditions via metabolomics, GC-MS, and luminescence assays. Alternative carbon sources and acetoacetate supplementation increase βHB. Mitochondrial acetyl-CoA metabolism and mtFAS pathways are identified as critical drivers. βHB supplementation extends chronological lifespan dose-dependently, and CR plus βHB produces additive effects. Transcriptomics reveal shared TCA and amino-acid responses and βHB-specific mitochondrial translation and stress pathways.",{"@graph":69,"@context":126},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/exploring-acetyl-coa-mediated-beta-hydroxybutyrate-enrichment-in-yeast-a-mechanistic-link-to-longevity/427704/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/exploring-acetyl-coa-mediated-beta-hydroxybutyrate-enrichment-in-yeast-a-mechanistic-link-to-longevity/427704.png","ImageObject",300,407,{"name":92,"@type":93},"\tCallum ","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-08","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118,122],{"name":109,"@type":110,"acceptedAnswer":111},"Why is this study focused on β-hydroxybutyrate (βHB) and caloric restriction (CR)?","Question",{"text":112,"@type":113},"CR extends lifespan across species, but its metabolic mechanisms are not fully understood. This study examines whether βHB is involved in CR-mediated longevity in yeast.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How did the researchers detect βHB in yeast despite the absence of canonical ketogenesis enzymes?",{"text":117,"@type":113},"They detect βHB in stationary-phase and CR conditions using metabolomics, GC-MS, and luminescence assays, showing yeast can produce or accumulate βHB under these states.",{"name":119,"@type":110,"acceptedAnswer":120},"What mechanisms and pathways were critical for βHB enrichment during CR?",{"text":121,"@type":113},"The study identifies mitochondrial acetyl-CoA metabolism via ACS1 and the mtFAS pathway as critical for enriching βHB during CR.",{"name":123,"@type":110,"acceptedAnswer":124},"How does βHB supplementation affect lifespan, and what does transcriptomic profiling reveal?",{"text":125,"@type":113},"βHB supplementation extends chronological lifespan dose-dependently, and combining CR with βHB gives additive extension. Transcriptomics show overlapping targets related to TCA cycle and amino acid metabolism, plus βHB-exclusive targets including mitochondrial translation and stress response pathways.","https://schema.org",{"og:url":83,"og:type":128,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":130,"canonical":83},"index,follow",{"doc_id":132,"site_id":62},427704,1790718391,{"code":4,"msg":5,"data":135},{"doc_id":132,"user_id":136,"nickname":92,"user_avatar":137,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":138,"file_id":139,"file_url":140,"file_type":141,"file_size":142,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":14,"language":143,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":144,"faqs":145,"seo_title":146,"seo_description":67,"update_tm":147,"read_time":24},137451211410,"https://ap-avatar.wpscdn.com/avatar/2000bb0a9246f588df?x-image-process=image/resize,m_fixed,w_180,h_180&k=1786362646172706240","747  \nAbstract citation ID: igaf122.2542  \nEXPLORING ACETYL-COA-MEDIATED  \nBETA-HYDROXYBUTYRATE ENRICHMENT IN YEAST: A MECHANISTIC LINK TO LONGEVITY  \n748 Innovation in Aging, 2025, Vol. 9, No. S2  \nSwarup Mishra1, Connor Oakes1, Elisa Enriquez Hesles1, and Jeffrey Smith2, 1. University of Virginia, Charlottesville, Virginia, United States, 2. University of Virginia School of Medicine, Charlottesville, Virginia, United States  \nAging predisposes organisms to diseases ranging from diabetes to Alzheimer’s, driving interest in interventions like Caloric Restriction (CR), which extends lifespan across species. While CR’s benefits are well-documented, its metabolic mechanisms remain incompletely understood. This study investigates β-hydroxybutyrate (βHB), a ketone body, in CR-mediated longevity using Saccharomyces cerevisiae. Despite the absence of canonical ketogenesis enzymes (HMG-CoA lyase, OXCT1) in yeast, we detect βHB for the first time in stationary-phase and CR conditions via metabolomics, GC-MS, and luminescence assays. Alternative carbon sources and supplementing yeast media with acetoacetate drive βHB levels. In addition, we identify mitochondrial acetyl-CoA metabolism (ACS1) and mtFAS pathways to be critical for βHB enrichment during CR. βHB supplementation extends chronological lifespan dose-dependently, mirroring CR’s effects, and combined CR + βHB yields additive lifespan extension. Transcriptomic profiling reveals 156 overlapping genes and 268 βHB-exclusive targets, suggesting shared (TCA cycle, amino acid metabolism) and βHB-specific mechanisms (mitochondrial translation, stress response). These findings resolve yeast’s unexpected capacity for ketogenesis and establish it as a model for dissecting βHB’s role in aging. This work also elucidates conserved nodes linking ketogenesis to longevity and positions yeast as a platform for exploring CR-mimicking therapeutics.","cbCaipivuQ1nXQwd","https://ap.wps.com/l/cbCaipivuQ1nXQwd","pdf",1306809,"English","# Background\n## Caloric restriction and lifespan mechanisms\n# Study Design and Methods\n## βHB detection across conditions\n## Metabolomics, GC-MS, and luminescence assays\n# Mechanistic Findings\n## Enzyme-independent ketogenesis signals\n## Role of acetyl-CoA metabolism and mtFAS\n## βHB enrichment drivers\n# Effects on Longevity\n## Dose-dependent lifespan extension\n## Additive impact of CR + βHB\n# Transcriptomic Insights\n## Overlapping genes and βHB-exclusive targets\n## Shared versus βHB-specific pathways","[{\"question\":\"Why is this study focused on β-hydroxybutyrate (βHB) and caloric restriction (CR)?\",\"answer\":\"CR extends lifespan across species, but its metabolic mechanisms are not fully understood. This study examines whether βHB is involved in CR-mediated longevity in yeast.\"},{\"question\":\"How did the researchers detect βHB in yeast despite the absence of canonical ketogenesis enzymes?\",\"answer\":\"They detect βHB in stationary-phase and CR conditions using metabolomics, GC-MS, and luminescence assays, showing yeast can produce or accumulate βHB under these states.\"},{\"question\":\"What mechanisms and pathways were critical for βHB enrichment during CR?\",\"answer\":\"The study identifies mitochondrial acetyl-CoA metabolism via ACS1 and the mtFAS pathway as critical for enriching βHB during CR.\"},{\"question\":\"How does βHB supplementation affect lifespan, and what does transcriptomic profiling reveal?\",\"answer\":\"βHB supplementation extends chronological lifespan dose-dependently, and combining CR with βHB gives additive extension. Transcriptomics show overlapping targets related to TCA cycle and amino acid metabolism, plus βHB-exclusive targets including mitochondrial translation and stress response pathways.\"}]","EXPLORING ACETYL-COA-MEDIATED BETA-HYDROXYBUTYRATE ENRICHMENT IN YEAST - A MECHANISTIC LINK TO LONGEVITY | PDF",1790644617]