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The electrode uses a layer-by-layer assembly of Nafion, Co-MOF nanosheets, CuO nanoparticles, and glucose oxidase (GOx). Structural and electrochemical studies (UV-vis, FTIR, DLS, zeta potential, XRD, Raman, SEM, TEM, EDS, XPS, CV, and EIS) confirm improved conductivity and a larger electroactive area. Differential pulse voltammetry provides a linear range of 2.0–14 mM and a limit of detection of 0.23 mM, with good repeatability, reproducibility (RSD = 3.9%), stability, and recovery (97–98.17%), while molecular docking supports specific Reb A–GOx interactions.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/exploring-a-genaonco-mof-nanosheetscuonpsgox-powered-electrochemical-biosensor-for-ultrasensitive-detection-of-rebaudioside-a/438558/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/exploring-a-genaonco-mof-nanosheetscuonpsgox-powered-electrochemical-biosensor-for-ultrasensitive-detection-of-rebaudioside-a/438558.png","ImageObject",300,407,{"name":92,"@type":93},"Himbo","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-01","2026-09-29",true,{"@type":102,"interactionType":103,"userInteractionCount":81},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"How is the electrochemical biosensor constructed for Reb A detection?","Question",{"text":112,"@type":113},"A modified graphite rod electrode is fabricated using a layer-by-layer strategy incorporating Nafion, Co-MOF nanosheets, CuO nanoparticles, and the GOx enzyme.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which techniques were used to characterize the nanomaterials and the sensor performance?",{"text":117,"@type":113},"The nanomaterials were characterized using UV-vis, FTIR, DLS, zeta potential, XRD, Raman, SEM, TEM, EDS, and XPS; performance was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), with DPV for quantitative response.",{"name":119,"@type":110,"acceptedAnswer":120},"What analytical performance does the biosensor achieve for Reb A?",{"text":121,"@type":113},"DPV shows linear response from 2.0–14 mM with a limit of detection of 0.23 mM. The sensor also demonstrates good repeatability and reproducibility (RSD = 3.9%), stability, and recovery results between 97% and 98.17%.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},438558,1790718391,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":81,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},687197100911,"https://ap-avatar.wpscdn.com/avatar/a000239b6f1da00475?x-image-process=image/resize,m_fixed,w_180,h_180&k=1785132997149421697","Nanoscale Advances  \nPAPER  \nCite this: DOI: 10 .1039/d5na00591d  \nReceived 16th June 2025  \nAccepted 7th September 2025 DOI: 10.1039/d5na00591d[rsc.li/nanoscale-advances](rsc.li/nanoscale-advances)  \nExploring a GE/Nafion/Co-MOF nanosheets/CuONPs/GOx powered electrochemical biosensor for ultrasensitive detection of rebaudioside A  \nManju Manuel, ab Suvardhan Kanchi  *ab and Venkatramana Losetty  c  \nRebaudioside A (Reb A) is a natural, non-nutritive sweetener highly prevalent in the global sweetener market and widely preferred by consumers. In this study, an advanced electrochemical biosensor was developed for sensing Reb A, using a modified graphite rod electrode extracted from discharged Zn–C batteries. The electrode was fabricated using a layer-by-layer strategy with Nafion, Co-MOF nanosheets, CuONPs, and glucose oxidase (GOx) enzyme. The nanomaterials were characterized by UV-vis, FTIR, DLS, zeta potential measurements, XRD, Raman, SEM, TEM, EDS, and XPS techniques. Electrochemical characterization via Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) revealed a significant enhancement in electrical conductivity and increased electroactive surface area. The designed biosensor exhibited a sharp oxidation peak at 0.16 V due to ester bond cleavage in Reb A, which was further amplified in the presence of GOx, resulting from hydroxyl oxidation and hydrogen peroxide generation. Differential pulse voltammetry (DPV) demonstrated a linear response over a concentration range of 2 .0–14 mM (R2 = 0. 993) with a limit of detection (LOD) of 0 .23 mM. The sensor displayed excellent analytical performance, with repeatability, reproducibility (RSD = 3. 9%), and stability. Additionally, recovery studies confirmed its accuracy, ranging from 97% to 98 . 17% . Further, the molecular docking studies confirmed strong Reb A–GOx interactions ( −7. 26 kcal mol −1), supporting the biosensor's specificity. The developed biosensor demonstrates excellent analytical performance, making it highly suitable for routine laboratory analysis of sweeteners in complex food matrices.  \n1. Introduction  \nNatural sweeteners have emerged as signi􀀁cant alternatives toarti􀀁cial sweeteners in the global food industry due to their low caloric content and potential health bene􀀁ts.1 Steviol glycosides, extracted from Stevia rebaudiana (Asteraceae), have gained considerable attention as natural sweetening agents.2,3 Rebaudioside A (Reb A) has attracted particular interest due to its superior organoleptic properties. Phytochemical analysis of wild S. rebaudiana leaves reveals a distribution of steviol glycosides comprising stevioside (9.1%), rebaudioside A (3.8%), rebaudioside C (0.6%), and glucosides (0.3%) .4 Among these glycosides, Reb A demonstrates enhanced sweetness intensity, improved stability, and reduced bitterness in comparison with stevioside.5,6 This improvement in properties is attributed to its structural characteristics, speci􀀁cally the substitution of a sophorosyl moiety with a glucosyl (1/3)-sophorosyl residue.7  \naDepartment of Chemistry, CHRIST University, Bengaluru, 560 029, India. E-mail: [ksuvardhan@gmail.com](ksuvardhan@gmail.com)  \nbCentre for Renewable Energy and Environmental Sustainability, CHRIST University, Bengaluru 560 029, India. E-mail: [suvardhan.k@christuniversity.in](suvardhan.k@christuniversity.in)  \ncDepartment of Chemistry, Vel Tech Rangarajan Dr Sagunthala R&D Institute of Science and Technology, Avadi, Chennai, 600 062, India  \nThe United States Food and Drug Administration (FDA) has granted steviol glycosides “Generally Recognised as Safe”(GRAS) status, with an Acceptable Daily Intake (ADI) of 4 mg per kg body weight.8 Consequently, the demand for Reb A has increased due to its therapeutic potential and applications in weight management.9 This increasing utilization in food products necessitates the development of effective analytical methods for its detection and quanti􀀁cation. Conventional analytical techniques for","cbCaitrMzBKBCirK","https://ap.wps.com/l/cbCaitrMzBKBCirK","pdf",2002273,14,"English","# Introduction\n## Background on natural sweeteners and Reb A\n## Analytical methods and their limitations\n## Motivation for rapid, sensitive electrochemical detection\n## Role of nanomaterials, MOFs, and enzymes in biosensing","[{\"question\":\"How is the electrochemical biosensor constructed for Reb A detection?\",\"answer\":\"A modified graphite rod electrode is fabricated using a layer-by-layer strategy incorporating Nafion, Co-MOF nanosheets, CuO nanoparticles, and the GOx enzyme.\"},{\"question\":\"Which techniques were used to characterize the nanomaterials and the sensor performance?\",\"answer\":\"The nanomaterials were characterized using UV-vis, FTIR, DLS, zeta potential, XRD, Raman, SEM, TEM, EDS, and XPS; performance was evaluated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), with DPV for quantitative response.\"},{\"question\":\"What analytical performance does the biosensor achieve for Reb A?\",\"answer\":\"DPV shows linear response from 2.0–14 mM with a limit of detection of 0.23 mM. The sensor also demonstrates good repeatability and reproducibility (RSD = 3.9%), stability, and recovery results between 97% and 98.17%.\"}]","Exploring a GE/Nafion/Co-MOF nanosheets/CuONPs/GOx powered electrochemical biosensor for ultrasensitive detection of rebaudioside A | PDF",1790685707,35]