[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-371669-105":59,"doc-detail-371669-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","exploring-a-circulating-circrna-and-mirna-biomarker-panel-for-early-detection-of-ovarian-cancer-through-multiple-omics-analysis","Exploring a circulating circRNA and miRNA biomarker panel for early detection of ovarian cancer through multiple omics analysis","","Circular RNAs (circRNAs) are widely studied as cancer biomarkers, yet their roles in ovarian cancer (OC) and value for early detection remain insufficiently defined. Using RNA sequencing on ovarian cancer cell lines, the study identifies circRNAs linked to OC and applies bioinformatics to clarify functional mechanisms. Receiver operating characteristic analyses assess discrimination, and circRNA-miRNA-mRNA networks suggest involvement in MAPK, Wnt, and ErbB signaling. Expression profiles are validated in cell, tissue, and plasma, forming a circRNA-miRNA detection panel that outperforms traditional markers for OC and early-stage discrimination in a cohort of 28 controls and 22 patients.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":35,"@type":76,"position":81},"https://docshare.wps.com/document/healthcare/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/exploring-a-circulating-circrna-and-mirna-biomarker-panel-for-early-detection-of-ovarian-cancer-through-multiple-omics-analysis/371669/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/exploring-a-circulating-circrna-and-mirna-biomarker-panel-for-early-detection-of-ovarian-cancer-through-multiple-omics-analysis/371669.png","ImageObject",300,407,{"name":92,"@type":93},"Đào","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-29","2026-09-23",true,{"@type":102,"interactionType":103,"userInteractionCount":44},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"Why are circRNAs considered promising biomarkers for ovarian cancer early detection?","Question",{"text":112,"@type":113},"circRNAs are structurally stable, conserved, and abundant, with tissue- and stage-specific expression. Their stability makes them resistant to degradation, supporting their potential usefulness in biomarker development for OC.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"How does the study identify circRNA candidates related to ovarian cancer?",{"text":117,"@type":113},"The work performs RNA sequencing on ovarian cancer cell lines to identify circRNAs correlated with OC. Functional mechanisms are then investigated using bioinformatics, including circRNA-miRNA-mRNA regulatory network construction and enrichment analyses.",{"name":119,"@type":110,"acceptedAnswer":120},"What biomarker panel was developed and how did it perform in ROC analysis?",{"text":121,"@type":113},"The study combines validated circRNAs and miRNAs into a circRNA and miRNA detection (CMD) panel. ROC results show superior discrimination for OC versus traditional protein-level biomarkers, and the panel exhibits exceptional potential for distinguishing early-stage OC from healthy controls.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},371669,1790246923,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":34,"category_name":35,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":44,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":139,"language":140,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":141,"faqs":142,"seo_title":143,"seo_description":67,"update_tm":144,"read_time":145},1374402968488,"https://ap-avatar.wpscdn.com/davatar_29158cc5080c5b710cf443261637dec0","[www. nature.com/scientificreports](www. nature.com/scientificreports)  \nOPEN  \nExploring a circulating circRNA and miRNA biomarker panel for early detection of ovarian cancer through multiple omics analysis  \nLingxi Tian1, Feng Xu2, Yang Lu1, Zaian Deng3􀀍, Yan Gao4 & Jun Yang1􀀍  \nThe biological functions of circular RNA (circRNAs) in cancers have garnered significant attention, particularly for their potential as biomarkers. However, the roles of circRNAs in ovarian cancer (OC) and their applicability for early detection of this malignancy remain underexplored. We performed RNA sequencing on ovarian cancer cell lines to identify circRNAs associated with OC. The functional mechanisms ofthe identified circRNAs were elucidated through bioinformatics analysis. The discriminating ability of biomarkers was assessed using receiver operating characteristic (ROC) analysis. RNA sequencing analysis revealed that 170 known circRNAs were correlated with ovarian cancer. Through the circRNA-miRNA-mRNA regulatory network, we identified 9 circRNAs that interact with 8 miRNAs, subsequently regulating the expression of 324 mRNAs. Functional enrichment analysis, protein-protein interaction (PPI) network analysis, and hub gene analysis indicated that these circRNAs and miRNAs may play a role in regulating MAPK, Wnt, and ErbB signaling pathways. We validated these circRNAs and miRNAs expression profiles in cell, tissue, and plasma samples, identifying four candidates—hsa_circ_ 0049101, hsa_circ_ 0007440, hsa_circ_ 0006935, and hsa-miR- 338-3p—that expression level positively correlate with ovarian cancer development. These markers were then combined into a circRNA and miRNA detection (CMD) panel for ovarian cancer detection. The area under the curve (AUC) values obtained from ROC analysis demonstrated that these individual candidates, as well as the CMD panel, exhibited superior discriminatory ability for OC compared to traditional biomarkers such as CA125, HE4, and the ROMA index in our sample set, which included 28 healthy controls and 22 ovarian cancer patients. Notably, the CMD panel showed exceptional potential for distinguishing early-stage OC samples from healthy controls, achieving anAUC of 1. In this study, we elucidated the functional mechanisms of a set ofcircRNAs associated with OC through multi-omics analysis and demonstrated that the combination ofcircRNAs and miRNAs into a biomarker panel holds significant potential for early detection of ovarian cancer.  \nKeywords Ovarian cancer, Biomarker, CircRNA-miRNA panel, Bioinformatics, Clinical early detection  \nAbbreviations  \nOCcircRNA DECs  \nPPI ceRNAGO  \nKEGG  \nROC RT-qPCRAUC  \nOvarian cancer Circular RNA  \nDifferentially expressed circRNAs Protein-protein interaction Competing endogenous RNA Gene ontology  \nKyoto encyclopedia of genes and genomes Receiver operating characteristic  \nQuantitative reverse transcriptase-polymerase chain reaction The area under the ROC curve  \n1MOE Key Laboratory of Intelligent Biomanufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China. 2Department of thoracic surgery, Dalian Municipal Central Hospital, Central Hospital of Dalian University of Technology, Dalian 116033, China. 3College of health science and environmental engineering, ShenZhen Technology University, ShenZhen 518118, China. 4Department of gynecology, Liaoning Cancer hospital & Institute, Shenyang 110044, China. 􀀍 email: [dengzaian@sztu.edu.cn](dengzaian@sztu.edu.cn); [junyang@dlut.edu.cn](junyang@dlut.edu.cn)  \n[www. nature.com/scientificreports/](www. nature.com/scientificreports/)  \nOvarian cancer (OC) is a malignant gynecological disease of the female reproductive system. It is the fifth leading cause of cancer death in women in the world1,2. Due to the lack of typical signs and symptoms, most patients have already been diagnosed with stage III (5%) or stage IV (51%) ovarian cancer at initial presentation and are accompanied by extensive peritoneal me","cbCaiuyjbFWZqZcj","https://ap.wps.com/l/cbCaiuyjbFWZqZcj","pdf",3706055,17,"English","# Abstract and background\n# circRNA biology and relevance to cancer\n# Study design and multi-omics workflow\n## RNA sequencing and circRNA discovery\n## Regulatory network and pathway analysis\n## Validation in samples and biomarker panel construction\n## ROC performance versus CA125, HE4, ROMA","[{\"question\":\"Why are circRNAs considered promising biomarkers for ovarian cancer early detection?\",\"answer\":\"circRNAs are structurally stable, conserved, and abundant, with tissue- and stage-specific expression. Their stability makes them resistant to degradation, supporting their potential usefulness in biomarker development for OC.\"},{\"question\":\"How does the study identify circRNA candidates related to ovarian cancer?\",\"answer\":\"The work performs RNA sequencing on ovarian cancer cell lines to identify circRNAs correlated with OC. Functional mechanisms are then investigated using bioinformatics, including circRNA-miRNA-mRNA regulatory network construction and enrichment analyses.\"},{\"question\":\"What biomarker panel was developed and how did it perform in ROC analysis?\",\"answer\":\"The study combines validated circRNAs and miRNAs into a circRNA and miRNA detection (CMD) panel. ROC results show superior discrimination for OC versus traditional protein-level biomarkers, and the panel exhibits exceptional potential for distinguishing early-stage OC from healthy controls.\"}]","Exploring a circulating circRNA and miRNA biomarker panel for early detection of ovarian cancer through multiple omics analysis | PDF",1790192574,43]