[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"detail-sidebar-cat-0-en-105":3,"doc-seo-664392-105":59,"doc-detail-664392-en":130},{"code":4,"msg":5,"data":6},0,"success",[7,13,18,23,28,33,38,43,48,51,55],{"id":8,"doc_module":4,"doc_module_name":9,"category_name":10,"show_sort_weight":11,"slug":12},1,"Document","Story & Novel",90,"story-novel",{"id":14,"doc_module":4,"doc_module_name":9,"category_name":15,"show_sort_weight":16,"slug":17},2,"Literature",80,"literature",{"id":19,"doc_module":4,"doc_module_name":9,"category_name":20,"show_sort_weight":21,"slug":22},4,"Exam",70,"exam",{"id":24,"doc_module":4,"doc_module_name":9,"category_name":25,"show_sort_weight":26,"slug":27},5,"Comic",60,"comic",{"id":29,"doc_module":4,"doc_module_name":9,"category_name":30,"show_sort_weight":31,"slug":32},6,"Technology",50,"technology",{"id":34,"doc_module":4,"doc_module_name":9,"category_name":35,"show_sort_weight":36,"slug":37},7,"Healthcare",40,"healthcare",{"id":39,"doc_module":4,"doc_module_name":9,"category_name":40,"show_sort_weight":41,"slug":42},8,"Research & Report",30,"research-report",{"id":44,"doc_module":4,"doc_module_name":9,"category_name":45,"show_sort_weight":46,"slug":47},9,"Religion & Spirituality",20,"religion-spirituality",{"id":46,"doc_module":4,"doc_module_name":9,"category_name":49,"show_sort_weight":46,"slug":50},"World Cup","world-cup",{"id":52,"doc_module":4,"doc_module_name":9,"category_name":53,"show_sort_weight":52,"slug":54},10,"Lifestyle","lifestyle",{"id":56,"doc_module":4,"doc_module_name":9,"category_name":57,"show_sort_weight":24,"slug":58},19,"General","general",{"code":4,"msg":60,"data":61},"ok",{"site_id":62,"language":63,"slug":64,"title":65,"keywords":66,"description":67,"schema_data":68,"social_meta":123,"head_meta":125,"extra_data":127,"updated_unix":129},105,"en","experimental-approach-in-molecular-biology-and-biochemistry-subject-description-form-abct3116","Experimental Approach in Molecular Biology and Biochemistry - Subject Description Form - ABCT3116","","Experimental Approach in Molecular Biology and Biochemistry (ABCT3116) is a laboratory-based course focused on designing, performing, and analyzing molecular biology and biochemical mini-projects. Students build deep understanding of DNA cloning principles, DNA analysis, site-directed mutagenesis, and protein expression analysis, then plan and execute experiments using PCR, restriction digestion, plasmid cloning vectors, transformation, protein expression workflows, gel electrophoresis, SDS-PAGE, and DNA sequencing. Learners develop technical skills, keep precise lab records, write reports, and present findings, integrating theory with safe, accurate experimental practice.",{"@graph":69,"@context":122},[70,84,105],{"@type":71,"itemListElement":72},"BreadcrumbList",[73,77,79,82],{"item":74,"name":75,"@type":76,"position":8},"https://docshare.wps.com","Home","ListItem",{"item":78,"name":9,"@type":76,"position":14},"https://docshare.wps.com/document/",{"item":80,"name":40,"@type":76,"position":81},"https://docshare.wps.com/document/research-report/",3,{"item":83,"name":65,"@type":76,"position":19},"https://docshare.wps.com/document/experimental-approach-in-molecular-biology-and-biochemistry-subject-description-form-abct3116/664392/",{"url":83,"name":65,"@type":85,"image":86,"author":91,"headline":65,"publisher":94,"fileFormat":97,"inLanguage":63,"description":67,"dateModified":98,"datePublished":99,"encodingFormat":97,"isAccessibleForFree":100,"interactionStatistic":101},"DigitalDocument",{"url":87,"@type":88,"width":89,"height":90},"https://docshare.wps.com/thumbnails/experimental-approach-in-molecular-biology-and-biochemistry-subject-description-form-abct3116/664392.png","ImageObject",300,407,{"name":92,"@type":93},"Taylor Morgan","Person",{"url":74,"name":95,"@type":96},"DocShare","Organization","application/pdf","2026-10-30","2026-10-29",true,{"@type":102,"interactionType":103,"userInteractionCount":8},"InteractionCounter",{"@type":104},"ViewAction",{"@type":106,"mainEntity":107},"FAQPage",[108,114,118],{"name":109,"@type":110,"acceptedAnswer":111},"What is the main learning focus of ABCT3116?","Question",{"text":112,"@type":113},"ABCT3116 centers on hands-on laboratory experience through a molecular biology/biochemical mini-project, including planning, executing experiments, analyzing results, and reporting findings.","Answer",{"name":115,"@type":110,"acceptedAnswer":116},"Which key techniques are used in the course?",{"text":117,"@type":113},"Students use methods including PCR-based site-directed mutagenesis, restriction digestion, plasmid cloning vectors, bacterial transformation for amplification and purification, protein expression and purification, DNA gel electrophoresis, protein SDS-PAGE, and DNA sequencing.",{"name":119,"@type":110,"acceptedAnswer":120},"How are students assessed and what deliverables are required?",{"text":121,"@type":113},"Assessment includes attendance, tutorial exercises, lab performance, lab proposal, quizzes, lab report, and a presentation. Students also must write an accurate laboratory notebook and submit a final written report and an oral presentation on site-directed mutagenesis.","https://schema.org",{"og:url":83,"og:type":124,"og:title":65,"og:site_name":95,"og:description":67},"article",{"robots":126,"canonical":83},"index,follow",{"doc_id":128,"site_id":62},664392,1793253316,{"code":4,"msg":5,"data":131},{"doc_id":128,"user_id":132,"nickname":92,"user_avatar":133,"doc_module":4,"category_id":39,"category_name":40,"doc_title":65,"doc_description":67,"doc_content":134,"file_id":135,"file_url":136,"file_type":137,"file_size":138,"view_count":8,"is_deleted":4,"is_public":8,"is_downloadable":8,"audit_status":8,"page_count":81,"language":139,"language_code":63,"site_id":62,"html_lang":63,"table_of_contents":140,"faqs":141,"seo_title":142,"seo_description":67,"update_tm":129,"read_time":39},1099523885336,"https://ap-avatar.wpscdn.com/davatar_276721f389ce27ea32af1340a28f341c","Subject Description Form  \n\n| Subject Code | ABCT3116 |\n| --- | --- |\n| Subject Title | Experimental Approach in Molecular Biology and Biochemistry |\n| Credit Value | 3 |\n| Level | 3 |\n| Pre-requisite | DNA Technology, Biochemical Techniques |\n| Objectives | This subject is a laboratory-based subject which will provide students a hands-on experience of designing, performing, and analyzing results from a molecular biology/biochemical mini-project. |\n| Intended Learning Outcomes | Upon completion of the subject, students will be able to:\u003Cbr>(a) Have a deep understanding of the principles of DNA cloning, DNA analysis, site directed mutagenesis, and protein expression analysis;\u003Cbr>(b) Plan and carry out a mini-project that require the above techniques;\u003Cbr>(c) Develop good technical skills, analyze collected data and present data;\u003Cbr>(d) Keep an exact record of the work performed and the results observed;\u003Cbr>(e) Present findings ofthe mini-project in the form of a written report.\u003Cbr>(f) Present a scientific article about site-directed mutagenesis by an oral presentation. |\n| Subject Synopsis/ Indicative Syllabus | Students will be presented with a problem or task that requires the use of at least the following molecular biology and biochemical techniques:\u003Cbr>(a) Site-directed mutagenesis using PCR, restriction digestion, plasmid cloning vectors;\u003Cbr>(b) Transformation into bacterial cells for amplification and purification from bacterial cells;\u003Cbr>(c) Protein expression, extraction, purification and characterizations;\u003Cbr>(d) DNA gel electrophoresis, Protein SDS-PAGE, DNA sequencing;\u003Cbr>(e) Study of protein modification and its implications\u003Cbr>Students will be asked to plan experiments and present it in the form of a proposal.\u003Cbr>Students will carry out the experiments involving the above techniques and collect data from their work.\u003Cbr>Students will meet tutors frequently to discuss the experiment progress and finalize details of coming laboratory work.\u003Cbr>Students will write up a report to present their work and findings.\u003Cbr>Students will present a scientific article in front of the whole class. |\n| Teaching/Learning Methodology | No formal lectures will be provided but a briefing session on the requirement of this subject and the assessment criteria. Students are expected to have learnt the basic principles of the experiments in the subjects of “DNA Technology” and“Biochemical Techniques”.\u003Cbr>Students will be asked to plan experiments to complete a task and present it in the form of a proposal.\u003Cbr>Students will carry out the experiments with provided manuals.\u003Cbr>Weekly one-hour tutorials will be held before the experiments for students to understand the theory of the experiments.\u003Cbr>Students are expected to write their own log books for preparation of the coming experiment.\u003Cbr>Students have to record the exact procedures used and to record the results obtained in the logbook.\u003Cbr>To conclude the subject, each student will prepare a written report on the experiments and the findings. |\n\n\n|  | Short quiz will be delivered to test students’ understanding of the experiments.\u003Cbr>Oral presentation is also required for students to present an article about site-directed mutagenesis and correlate with the knowledge learnt from the course. |  |  |  |  |  |  |  |  |  |  |\n| --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |\n| Assessment Methods in Alignment with Intended Learning Outcomes |  | Specific assessment methods/tasks | %\u003Cbr>weighting |  | Intended subject learning outcomes to be assessed (Please tick as appropriate) |  |  |  |  |  |  |\n|  |  |  |  |  | a | b | c | d | e | f |  |\n|  |  | 1. Attendance | 10 |  | 􀀹 |  | 􀀹 |  |  |  |  |\n|  |  | 2. Tutorial exercise | 10 |  | 􀀹 |  | 􀀹 |  |  |  |  |\n|  |  | 3. Lab performance | 20 |  | 􀀹 | 􀀹 |  |  |  |  |  |\n|  |  | 4. Lab proposal | 15 |  | 􀀹 | 􀀹 | 􀀹 | 􀀹 |  |  |  |\n|  |  | 5. Lab quizzes | 10 |  | 􀀹 |  |  |  |  |  |  |\n|  |  | 6. Lab report | 25 |  | 􀀹 |  | 􀀹 |  | 􀀹 |","cbCaimW5subN4XXK","https://ap.wps.com/l/cbCaimW5subN4XXK","pdf",172966,"English","# Subject Description Form\n## Course Details\n## Objectives and Intended Learning Outcomes\n## Subject Synopsis / Indicative Syllabus\n## Teaching/Learning Methodology\n## Assessment Methods and Alignment","[{\"question\":\"What is the main learning focus of ABCT3116?\",\"answer\":\"ABCT3116 centers on hands-on laboratory experience through a molecular biology/biochemical mini-project, including planning, executing experiments, analyzing results, and reporting findings.\"},{\"question\":\"Which key techniques are used in the course?\",\"answer\":\"Students use methods including PCR-based site-directed mutagenesis, restriction digestion, plasmid cloning vectors, bacterial transformation for amplification and purification, protein expression and purification, DNA gel electrophoresis, protein SDS-PAGE, and DNA sequencing.\"},{\"question\":\"How are students assessed and what deliverables are required?\",\"answer\":\"Assessment includes attendance, tutorial exercises, lab performance, lab proposal, quizzes, lab report, and a presentation. Students also must write an accurate laboratory notebook and submit a final written report and an oral presentation on site-directed mutagenesis.\"}]","Experimental Approach in Molecular Biology and Biochemistry - Subject Description Form - ABCT3116 | PDF"]